Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,538,814 C→T 7.4% T477M (ACG→ATG)  yhgF → putative transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,538,8140CT 7.4% 163.1 / 3.1 54T477M (ACG→ATG) yhgFputative transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base C (21/29);  new base T (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

TGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGAT  >  NC_000913/3538671‑3538959
                                                                                                                                               |                                                                                                                                                 
tgCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAAc                                                                                                                                              >  2:7683/1‑149 (MQ=255)
  cacaGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACt                                                                                                                                             <  1:4698/148‑1 (MQ=255)
   acaGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCACCTgg                                                                                                                                           >  8:207089/1‑149 (MQ=255)
      ggAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGccc                                                                                                                                        <  6:229598/149‑1 (MQ=255)
               ggATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGATGCAACTGGCCCGCAAACTgg                                                                                                                               >  3:32065/1‑149 (MQ=255)
               ggATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGATGCAACTGGCCCGCAAACTg                                                                                                                                >  3:189320/1‑148 (MQ=255)
                gATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTCTCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGa                                                                                                                              <  4:157838/149‑1 (MQ=255)
                  tctcGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACg                                                                                                                            >  7:163572/1‑149 (MQ=255)
                      gACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAACTCGATCCGAAATCTATCGGCGTAGGTCAGTATCCGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCagt                                                                                                                        <  2:188797/149‑1 (MQ=255)
                      gACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCag                                                                                                                         <  3:33130/148‑1 (MQ=255)
                         gTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCagtag                                                                                                                      >  6:67326/1‑148 (MQ=255)
                              gCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTagaa                                                                                                                 <  6:16983/148‑1 (MQ=255)
                                         cgGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAAc                                                                                                     <  7:169120/149‑1 (MQ=255)
                                            tgtCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGcc                                                                                                  >  7:133564/1‑149 (MQ=255)
                                               cTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGtt                                                                                               >  8:54840/1‑149 (MQ=255)
                                                 aTCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTgg                                                                                             >  1:123040/1‑149 (MQ=255)
                                                  tCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGtt                                                                                               <  3:147755/146‑1 (MQ=255)
                                                  tCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTgg                                                                                             <  8:15154/148‑1 (MQ=255)
                                                  tCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTg                                                                                              <  3:3738/147‑1 (MQ=255)
                                                        gccgTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGAt                                                                                      <  6:204031/149‑1 (MQ=255)
                                                           gTTTGCAGGATCCGCTGGCTGAGCTGGTGAAAATCGCTCCGAAATCTATCGGCGGAGGTCAGGATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCCAACTGGACGCAGTAGTAGAAGACTGCGTAACCGCCGTTGGCGTCGAtct                                                                                    >  1:246012/1‑148 (MQ=255)
                                                               gCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGc                                                                                                                                      <  3:113736/94‑1 (MQ=255)
                                                               gCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGc                                                                                                                                      >  4:113736/1‑94 (MQ=255)
                                                                   gATCCGCTTGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGc                                                                           <  5:50134/149‑1 (MQ=255)
                                                                   gATCCGCTGGCGGAGCTTGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCATCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACttc                                                                           <  5:50136/149‑3 (MQ=255)
                                                                   gATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGAtctc                                                                                   <  1:103839/141‑1 (MQ=255)
                                                                   gATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGAtctc                                                                                   >  2:103839/1‑141 (MQ=255)
                                                                   gATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGc                                                                           >  8:180014/1‑149 (MQ=255)
                                                                       cGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTc                                                                        <  7:54840/148‑1 (MQ=255)
                                                                        gCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTg                                                                      <  1:50760/149‑1 (MQ=255)
                                                                        gCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACCCAGTAGTAGAAGACTGCGAAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTg                                                                      >  8:196703/1‑149 (MQ=255)
                                                                            gCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTc                                                                   <  8:133564/148‑1 (MQ=255)
                                                                              ggAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGc                                                                <  2:18978/149‑1 (MQ=255)
                                                                                      tGAAAATAGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAAcc                                                        <  8:2255/149‑1 (MQ=255)
                                                                                         aaaTCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCcgc                                                     >  4:58729/1‑149 (MQ=255)
                                                                                         aaaTCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCcgc                                                     <  1:7683/149‑1 (MQ=255)
                                                                                              gATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTgg                                                 <  2:123040/148‑1 (MQ=255)
                                                                                               aTCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGc                                                                           <  3:185027/121‑1 (MQ=255)
                                                                                               aTCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGc                                                                           >  4:185027/1‑121 (MQ=255)
                                                                                               aTCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCg                                               >  7:86934/1‑149 (MQ=255)
                                                                                                  cGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACCCTGCTTCTGTTCCGCTGTTAACCCGCGTGGCggg                                             <  7:90130/148‑1 (MQ=255)
                                                                                                  cGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGc                                            >  6:105628/1‑149 (MQ=255)
                                                                                                   gAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGcc                                           >  5:232563/1‑149 (MQ=255)
                                                                                                               cGTAGGTCAGTCTCAGCATTCCGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTTGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCat                                 <  2:246012/147‑1 (MQ=255)
                                                                                                                gTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCatgat                              >  1:90127/1‑149 (MQ=255)
                                                                                                                  aGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGcc                                           <  2:23918/134‑1 (MQ=255)
                                                                                                                  aGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGcc                                           >  1:23918/1‑134 (MQ=255)
                                                                                                                   ggTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGgc                           <  6:242419/149‑1 (MQ=255)
                                                                                                                             aGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATc                 <  6:232563/149‑1 (MQ=255)
                                                                                                                              gCATGACGTCAGCCAGATGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCg                <  4:189320/149‑1 (MQ=255)
                                                                                                                              gCATGACGTCAGCCAGATGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCg                <  4:32065/149‑1 (MQ=255)
                                                                                                                                    cGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCt          <  5:105628/149‑1 (MQ=255)
                                                                                                                                         gCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGgcgc     >  4:51182/1‑149 (MQ=255)
                                                                                                                                            aGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGAt  >  6:214753/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGAT  >  NC_000913/3538671‑3538959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑