Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,446,459 C→T 7.3% T783M (ACG→ATG)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,446,4590CT 7.3% 166.8 / 2.7 55T783M (ACG→ATG) tamBtranslocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (31/20);  new base T (2/2);  total (33/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

CTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCC  >  NC_000913/4446317‑4446606
                                                                                                                                              |                                                                                                                                                   
cTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGAAAGCGGTATATTCACGGGTAAAGCTGACGTTTCCTGGGACACCACTAAAGAGGGGCTGACGAAGGGCAGTATAACACTTTCGCGGCGTAACGTGCATGTAACTCAAAc                                                                                                                                               >  8:63981/1‑149 (MQ=255)
 tCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAccc                                                                                                                                              >  3:125079/1‑149 (MQ=255)
     cATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACTAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTc                                                                                                                                           >  4:1023/1‑148 (MQ=255)
     cATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCa                                                                                                                                          <  8:204374/149‑1 (MQ=255)
          tGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGAt                                                                                                                                     >  3:110247/1‑149 (MQ=255)
           gAAACTGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATg                                                                                                                                    >  4:199280/1‑149 (MQ=255)
                 gTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGgcgc                                                                                                                              >  6:140191/1‑149 (MQ=255)
                  tttATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCt                                                                                                                             <  3:4840/149‑1 (MQ=255)
                      tGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAAc                                                                                                                                                     <  7:146995/121‑1 (MQ=255)
                      tGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAAc                                                                                                                                                     >  8:146995/1‑121 (MQ=255)
                      tGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGccc                                                                                                                         <  5:140191/149‑1 (MQ=255)
                        ccAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGCCGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGt                                                                                                                       >  5:213782/1‑149 (MQ=255)
                        ccAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGt                                                                                                                       <  4:110247/149‑1 (MQ=255)
                         cAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTg                                                                                                                      >  1:63535/1‑149 (MQ=255)
                          aGAAAGCACTAAGGCCAGCGGTCTCTTCACTGGTAAAGCTGTCGTTGCCTGGGACACCCCGAAAGAGGGGCTGACGCAGGGCAGTATCAGCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTg                                                                                                                      <  1:185821/148‑1 (MQ=255)
                          aGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTgg                                                                                                                     >  1:17220/1‑149 (MQ=255)
                                 aCTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCa                                                                                                              <  2:170923/149‑1 (MQ=255)
                                                 tCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAATGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcg                                                                                              >  4:217392/1‑149 (MQ=255)
                                                 tCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcg                                                                                              >  3:219375/1‑149 (MQ=255)
                                                           tAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGc                                                                                    <  2:6339/149‑1 (MQ=255)
                                                             aaGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCTCCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCg                                                                                   <  1:169567/148‑1 (MQ=255)
                                                              aGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAATGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTa                                                                                 <  3:217392/149‑1 (MQ=255)
                                                              aGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGCCGTTTCAGACACTGAATCTGACGGCGGAATTGCGt                                                                                  >  4:200647/1‑148 (MQ=255)
                                                                     gTTGCCTGGGACACCCCGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACCCTGAATCTGACGGCGGAATTGCGTAACAAcct                                                                          >  8:33202/1‑148 (MQ=255)
                                                                     gTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCg                                                                          >  2:194698/1‑149 (MQ=255)
                                                                     gTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCg                                                                          >  8:29229/1‑149 (MQ=255)
                                                                                caccacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTgg                                                               <  3:200647/149‑1 (MQ=255)
                                                                                 accacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTggg                                                              >  8:236181/1‑149 (MQ=255)
                                                                                   cacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGCCACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCt                                                            >  3:124003/1‑149 (MQ=255)
                                                                                   cacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCt                                                            >  6:154161/1‑149 (MQ=255)
                                                                                   cacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCt                                                            >  1:6650/1‑149 (MQ=255)
                                                                                   cacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAAGTGGGCt                                                            >  8:187801/1‑149 (MQ=255)
                                                                                    acGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTg                                                           >  1:137191/1‑149 (MQ=255)
                                                                                    acGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTg                                                           <  6:213782/149‑1 (MQ=255)
                                                                                       aaaGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGAc                                                        <  2:217370/149‑1 (MQ=255)
                                                                                         agagGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACCCTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCa                                                      >  7:201679/1‑149 (MQ=255)
                                                                                         agagGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCa                                                      >  7:205189/1‑149 (MQ=255)
                                                                                               gCTGCCGCAGGGCAGTATCACCCTTGCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGcc                                                >  7:40444/1‑149 (MQ=255)
                                                                                                cTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCt                                               <  4:125079/149‑1 (MQ=255)
                                                                                                   ccGCATGGCAGTATCACCCTTTCGGGGCGTAACGGGCAGGTAACGCAAAACGTCAACGATGCGGCGCTGCCCGTGGAGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCAGAATTGGGCTGGACCATCCGCCTg                                              <  7:63981/147‑1 (MQ=255)
                                                                                                    cGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCACGTGGCGTTTCAGACACTGAATCTGACGTCGGAATTTCGTAACCACCGTGCCGAATTGGGCTGGACCATCCGCCTgcc                                            >  2:152103/1‑146 (MQ=255)
                                                                                                              tATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCCACCCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGc                                  >  5:105916/1‑148 (MQ=255)
                                                                                                              tATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGc                                  >  8:110674/1‑148 (MQ=255)
                                                                                                                tCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCTGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCa                                <  2:63535/148‑1 (MQ=255)
                                                                                                                tCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCa                                <  2:17220/148‑1 (MQ=255)
                                                                                                                  aCCCTTTCGGGGCGTAACGTGCAGGTAATGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCAt                                                     <  8:66033/125‑1 (MQ=255)
                                                                                                                  aCCCTTTCGGGGCGTAACGTGCAGGTAATGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCAt                                                     >  7:66033/1‑125 (MQ=255)
                                                                                                                  aCCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGaa                                                                                        >  8:196158/1‑90 (MQ=255)
                                                                                                                    ccTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGttt                            >  1:15766/1‑148 (MQ=255)
                                                                                                                        tCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGAt                         <  4:231981/147‑1 (MQ=255)
                                                                                                                        tCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGGGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGAt                         <  2:137191/147‑1 (MQ=255)
                                                                                                                          ggggCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGAc                     <  8:115111/149‑1 (MQ=255)
                                                                                                                            ggCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGAcag                   <  3:1023/149‑1 (MQ=255)
                                                                                                                                    gTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTggg                                                              >  6:117411/1‑98 (MQ=255)
                                                                                                                                    gTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTggg                                                              <  5:117411/98‑1 (MQ=255)
                                                                                                                                      gCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGa         >  2:124019/1‑149 (MQ=255)
                                                                                                                                             aCGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAGCGGCCAGTTTGATGGACAGGTGCAGGTGACCGATcc  >  2:124663/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCC  >  NC_000913/4446317‑4446606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑