Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,198,497 A→C 8.9% intergenic (‑260/‑198) ymfE ← / → lit e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,198,4970AC 8.9% 118.9 / 5.0 45intergenic (‑260/‑198)ymfE/lite14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage
Reads supporting (aligned to +/- strand):  ref base A (20/21);  new base C (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

GACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATT  >  NC_000913/1198355‑1198642
                                                                                                                                              |                                                                                                                                                 
gacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATgaaatgatgaaatgatgaaatgattaaatgattaaatgatgaaattat                                                                                                                                             >  8:121966/1‑149 (MQ=255)
gacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                                                                                                                                             <  8:190230/149‑1 (MQ=255)
gacaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                                                                                                                                             <  3:43130/149‑1 (MQ=255)
 acaATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                                                                                                                                             >  6:192185/1‑148 (MQ=255)
       ttGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACaga                                                                                                                                       <  4:203401/148‑1 (MQ=255)
       ttGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACaga                                                                                                                                       <  4:30868/148‑1 (MQ=255)
          tgATCAGTAGCACGTATCGAGGAGGAGTATCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAgtgt                                                                                                                                   <  4:31549/149‑1 (MQ=255)
                   gCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAAAGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTggg                                                                                                                           >  3:186451/1‑148 (MQ=255)
                      cGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACg                                                                                                                       <  6:217931/149‑1 (MQ=255)
                      cGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATA‑‑‑‑‑‑‑‑ATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGgtgt                                                                                                               <  6:77902/149‑1 (MQ=255)
                        tATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGa                                                                                                                     >  4:69686/1‑149 (MQ=255)
                          tCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATg                                                                                                                   >  7:107711/1‑149 (MQ=255)
                              ggaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAAAGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGgtgt                                                                                                               <  4:186451/149‑1 (MQ=255)
                               gaggagTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAATTGATGAAATGATGAAATGATGAAATCATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTc                                                                                                              >  3:5728/1‑149 (MQ=255)
                                aggagTATCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCt                                                                                                             <  2:25683/149‑1 (MQ=255)
                                   agTAGCGCTACAAATTTGACGCTGGGTGAGAATCTTAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTAc                                                                                                          <  8:107711/149‑1 (MQ=255)
                                     tAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGa                                                                                                        <  7:164823/149‑1 (MQ=255)
                                     tAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGa                                                                                                        <  1:247317/149‑1 (MQ=255)
                                       gcgcTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACa                                                                                                      <  3:69686/149‑1 (MQ=255)
                                         gcTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGTTGTCTTACGACAt                                                                                                     >  3:209642/1‑148 (MQ=255)
                                           tACAAATTTGACGCTGGGTGAGAATCTGAAATTTATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACaga                                                                                                                                       <  3:18013/112‑1 (MQ=255)
                                           tACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAATTGATGAAATGATGAAATGATGATATGATGAAATGATGAAAGAGTGTCCAGTGGGCACGGATGGAGTCTTACGACATGCt                                                                                                  >  7:212251/1‑149 (MQ=255)
                                           tACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACaga                                                                                                                                       >  4:18013/1‑112 (MQ=255)
                                              taaTTTGACGCTGGGTGATAATCTTTAATTTATATAAATGAAAAAATGAAATAATTAAATTATGAAATGATTAAATGATGAAATGATGTATTGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTAc                                                                                               <  8:212251/148‑1 (MQ=25)
                                              aaaTTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTAc                                                                                               <  2:107325/149‑1 (MQ=255)
                                                aTTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCt                                                                                             >  1:96724/1‑149 (MQ=255)
                                                      cGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTACAGTGGGAACGTATGGTGTCTTACGACATGCTTAACTTAATCg                                                                                       >  1:159243/1‑149 (MQ=255)
                                                         tGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTc                                                                                    >  6:144510/1‑149 (MQ=255)
                                                          gggTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTcc                                                                                   <  8:44738/149‑1 (MQ=255)
                                                           ggTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAATTGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGTGCACGGATGGTGTCTTACGACATGCTTACCTTTATCGTTcc                                                                                   >  1:222361/1‑148 (MQ=255)
                                                            gttataaTCTGAAATTTTTAGAAATGAAATAATTAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCa                                                                                 <  4:209642/144‑1 (MQ=255)
                                                                      gAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCta                                                                        >  1:52787/1‑148 (MQ=255)
                                                                      gAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCACAGTGTGCCta                                                                        >  2:19679/1‑148 (MQ=255)
                                                                          ttGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGCAATGATGACAGAGTGTCCAGTGTGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAg                                                                     <  2:53439/147‑1 (MQ=255)
                                                                          ttGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGCAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAg                                                                     >  1:141996/1‑147 (MQ=255)
                                                                          ttGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGCAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAg                                                                     <  2:141996/147‑1 (MQ=255)
                                                                          ttGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGCAATGATGACAGAGTGTCCAGTGAGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAg                                                                     >  1:53439/1‑147 (MQ=255)
                                                                             aTAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGGCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCGTAATCGTTCCCAGTGTGCCTATAGCAGa                                                                 >  6:135831/1‑148 (MQ=255)
                                                                              tAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGata                                                               >  7:56695/1‑149 (MQ=255)
                                                                                 aaatgaaatAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTc                                                            >  1:22911/1‑149 (MQ=255)
                                                                                    tgaaataatgaaataatgaaatGATGATAAGATCATATGATGAAATGATGAAATGAAGAAATGCTGACACAGTGACCAGTGGGAACGGAAGGTGTCTTACTACATGATTACCTTAATCCTTCCCAGTGTGCATATAGCAGCTATTcaa                                                          >  4:171529/1‑146 (MQ=25)
                                                                                    tgaaataatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTaa                                                         <  6:33598/149‑1 (MQ=255)
                                                                                      atttaatgaaatattgatatGATTATCTGATGATATGATGAAATTATGTAATGATGATATGATGACAGTGTGTCCAGTGGGCACGGATGGATTCTTACGAGATGCTTACATTATTCTTTCTCAGTGTGCCTATAGCAGATATTCTaaa                                                        <  5:84068/145‑1 (MQ=255)
                                                                                      aaataatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATTGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTaaa                                                        <  2:2718/148‑1 (MQ=255)
                                                                                        ataatgaaataatgaaatGATGAAATGATGAAATGATGATATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGAGATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACAt                                                     <  6:204309/149‑1 (MQ=255)
                                                                                          aatgaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGCGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTCCCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGt                                                   <  4:138648/149‑1 (MQ=255)
                                                                                                attatgaaatgatgaaatgatgaaatgatgtaattatgaaatgatgaaatgATGACAGTGTGTCCAGTGGGCAAGGATTGTGTCTTAAGACATGCTTACCTATATCGTTCCCAGTGTTCCTATAGCATATATTCTAAACATGTCGATaa                                             <  8:150774/146‑1 (MQ=37)
                                                                                                 taatgatatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAAt                                            >  3:8952/1‑149 (MQ=255)
                                                                                                 taatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAAt                                            >  4:70861/1‑149 (MQ=255)
                                                                                                  aatgaaatgaTGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAAt                                            >  4:87728/1‑148 (MQ=255)
                                                                                                   atgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTc                                          <  7:121966/149‑1 (MQ=255)
                                                                                                   atgaaatgatgaaataatgaaatgatgaaatgatgaaatgatgaaatgATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAAtt                                           <  4:141057/148‑1 (MQ=255)
                                                                                                    tgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgaTGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCa                                         >  4:200822/1‑149 (MQ=255)
                                                                                                       aatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTa                                      <  2:96724/149‑1 (MQ=255)
                                                                                                        atgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTAc                                     <  2:52787/149‑1 (MQ=255)
                                                                                                            tgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTc                                                            >  3:53660/1‑122 (MQ=255)
                                                                                                            tgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTc                                                            <  4:53660/122‑1 (MQ=255)
                                                                                                             gaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCata                                >  3:30982/1‑149 (MQ=255)
                                                                                                               aatgatgaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCAtata                              <  3:200822/149‑1 (MQ=255)
                                                                                                                atgatgaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGCCATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAg                             >  6:148405/1‑149 (MQ=255)
                                                                                                                    tgaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAGTATc                         <  5:235237/149‑1 (MQ=255)
                                                                                                                     gaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAGTATc                         >  3:65467/1‑148 (MQ=255)
                                                                                                                     gaaatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAGTATc                         >  3:16752/1‑148 (MQ=255)
                                                                                                                       aatgatgaaatgatgaaatgatgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAGTATCGaa                      <  1:19679/149‑1 (MQ=255)
                                                                                                                           atgaaatcatgaaatgatgaaattatgaCACAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTACCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAGTATCGAACATa                  <  2:222361/149‑1 (MQ=255)
                                                                                                                                            tgaaatgatgaCAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGAtt  >  8:4008/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GACAATATTGTGATCAGTAGCACGTATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTGGGCACGGATGGTGTCTTACGACATGCTTACCTTAATCGTTCCCAGTGTGCCTATAGCAGATATTCTAAACATGTCGATAATTCATTACGCATATAGTATCGAACATAGAAAAAACTGAAGATT  >  NC_000913/1198355‑1198642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑