Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503	1979278	776	32 [0]	[0] 33	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	32 (1.030)	28/262	0.2	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGC  >  NC_000913/1978357‑1978641                                                                                                                                                  |                                                                                                                                            aaaTGGGTCTTTACACTTCTCCAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATCACACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCTGATCACACAAGGCACTCAATTACTTAACATAATTGta                                                                                                                                           >  6:132293‑M1/1‑146 (MQ=255)   aTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                        <  6:10912‑M1/149‑6 (MQ=255)        tCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtca                                                                                                                                    >  3:94645‑M1/1‑139 (MQ=255)          tttAGACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTAGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTACAATAATGCGTGATGCAGTTCACACACAACACTCAATTACTTAACATAAATGtataattcatac                                                                                                                                 >  6:42496‑M1/1‑137 (MQ=255)               acTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttg                                                                                                                            >  2:5850‑M1/1‑132 (MQ=255)                cTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGCTCACACAAAACCCTCAATTACTTACCATAAATGtataagtcatacttttgt                                                                                                                           >  2:25746‑M1/1‑131 (MQ=255)                   aTCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtttt                                                                                                                        >  7:52515‑M1/1‑128 (MQ=255)                                    aaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGTGGGCTGCGATTTTCAATAATGCTTGATGCAGATCACACAAAACACTCAATTACTTAACATAAAAGtataagacatactattgttttgggtgtatttcaatctg                                                                                                       >  7:133678‑M1/1‑111 (MQ=255)                                            cAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaag                                                                                               <  3:131032‑M1/149‑47 (MQ=255)                                                   aCTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcg                                                                                        >  5:57500‑M1/1‑96 (MQ=255)                                                     tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgc                                                                                       >  1:139483‑M1/1‑94 (MQ=255)                                                         cACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc                                                                                  <  2:139483‑M1/149‑60 (MQ=255)                                                                         accggAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                  <  8:133678‑M1/147‑76 (MQ=255)                                                                           cGGAGGGGGGCTGAGATTTTCAATAATGCGAGTTGCAGATCACACAATACACTCAATTACTTAACAAAAATGtataagtcaaacttttgtttagggtctatttcaatctgttaacaagtttttcgctacgctagcaagcaaaaatgaaa                                                                <  5:42496‑M1/149‑78 (MQ=255)                                                                            ggAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaac                                                               <  1:5850‑M1/149‑79 (MQ=255)                                                                                  gggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactgttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaa                                                          >  4:74440‑M1/1‑65 (MQ=255)                                                                                    gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataa                                                       <  6:57500‑M1/149‑87 (MQ=255)                                                                                        cGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                     <  4:94645‑M1/147‑89 (MQ=255)                                                                                          aTTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaa                                                 <  8:25105‑M1/149‑93 (MQ=255)                                                                                                          gATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgca                                   >  1:93588‑M1/1‑41 (MQ=255)                                                                                                          gATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc                                                                                  <  6:105238‑M1/100‑60 (MQ=255)                                                                                                            tGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgtgtgtatttcaatctgttaaaatgtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagt                               <  4:80371‑M1/149‑111 (MQ=255)                                                                                                                 aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                          >  2:101684‑M1/1‑34 (MQ=255)                                                                                                                   cacacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaat                         >  3:80237‑M1/1‑32 (MQ=255)                                                                                                                      acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacgggaataatcgaaatgggatgttgcgcacagtcaaaataac                      >  7:129348‑M1/1‑29 (MQ=255)                                                                                                                             acTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgt               >  5:71360‑M1/1‑22 (MQ=255)                                                                                                                             acTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcacacagtcaaaataactcaccgt               >  6:65477‑M1/1‑22 (MQ=255)                                                                                                                              cTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaa             <  4:80237‑M1/149‑129 (MQ=255)                                                                                                                                 aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaattggatgtggcgcacagtcaaaataactcaccgtaaat           >  5:133608‑M1/1‑18 (MQ=255)                                                                                                                                 aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaat           >  5:65132‑M1/1‑18 (MQ=255)                                                                                                                                 aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaat           >  7:37540‑M1/1‑18 (MQ=255)                                                                                                                                          aCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctg  <  8:52515‑M1/148‑140 (MQ=255)                                                                                                                                                  |                                                                                                                                            AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGC  >  NC_000913/1978357‑1978641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑