BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,175,146	Δ1 bp	100%	intergenic (‑120/‑242)	coaA ← / → thrU	pantothenate kinase/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,146	0	A	.	99.1%	509.5 / ‑1.8	112	intergenic (‑120/‑242)	coaA/thrU	pantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (58/53); total (59/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 115 total aligned reads displayed.

ATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGT  >  NC_000913/4175001‑4175288

aTTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaa                                                                                                                                              >  8:26862/1‑148 (MQ=255)

aTTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaa                                                                                                                                              <  5:104246/148‑1 (MQ=255)

      gTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATctct                                                                                                                                      <  6:174821/149‑1 (MQ=255)

      gTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATctct                                                                                                                                      <  3:148690/149‑1 (MQ=255)

         tGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGc                                                                                                                                    <  2:193558/148‑1 (MQ=255)

          gCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGc                                                                                                                                    >  2:392407/1‑147 (MQ=255)

            tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAACGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAg                                                                                                                                 >  1:138818/1‑148 (MQ=255)

            tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAg                                                                                                                                 >  2:170229/1‑148 (MQ=255)

            tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAg                                                                                                                                 >  1:4667/1‑148 (MQ=255)

            tctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGAGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAg                                                                                                                                 >  4:50026/1‑148 (MQ=255)

              ttttATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGTGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTgagagg                                                                                                                                >  8:148949/1‑142 (MQ=255)

              ttttATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAgg                                                                                                                                >  3:56751/1‑147 (MQ=255)

                 tataCTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGc                                                                                                                            <  1:311012/148‑1 (MQ=255)

                 tataCTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGc                                                                                                                            <  4:56751/148‑1 (MQ=255)

                  ataCTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATctct                                                                                                                                      <  6:95172/137‑1 (MQ=255)

                  ataCTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATctct                                                                                                                                      >  5:95172/1‑137 (MQ=255)

                      tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                      >  6:383407/1‑149 (MQ=255)

                      tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                      >  3:126097/1‑149 (MQ=255)

                        aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTACCACAAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGtt                                                                                                                     >  6:271185/1‑148 (MQ=255)

                        aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAg                                                                                                                             <  1:83393/140‑1 (MQ=255)

                        aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAg                                                                                                                             >  2:83393/1‑140 (MQ=255)

                        aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGtt                                                                                                                     <  5:224807/148‑1 (MQ=255)

                        aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTGGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                      <  1:80094/147‑1 (MQ=255)

                           aGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGaa                                                                                                                  >  5:25098/1‑148 (MQ=255)

                            gCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGaaaa                                                                                                                <  8:407940/149‑1 (MQ=255)

                            gCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGaa                                                                                                                  >  8:45290/1‑147 (MQ=255)

                             cATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAg                                                                                                               >  7:231078/1‑149 (MQ=255)

                             cATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTGAATGGGCAGGAGGGTAACACCAGATTGGGAGGAATAATAAG‑AAAAATATCTGCCAGGAAGCTATCGTTGAAAAg                                                                                                               >  2:319217/1‑149 (MQ=255)

                             cATGTTTCTGGCGGACATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGAGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGATGGTAACCCCAGATTGGCAGGAATAATAAG‑AAAATTCTCTGCCAGGAAGCTAACGTTAAAAAg                                                                                                               >  2:279445/1‑149 (MQ=255)

                               tGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGc                                                                                                              >  1:137375/1‑148 (MQ=255)

                               tGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCa                                                                                                             <  8:231078/149‑1 (MQ=255)

                                 tttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                           <  1:228899/149‑1 (MQ=255)

                                 tttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                           <  1:271483/149‑1 (MQ=255)

                                    cTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtgt                                                                                                         <  2:4667/148‑1 (MQ=255)

                                    cTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtgt                                                                                                         <  1:392407/148‑1 (MQ=255)

                                       gCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCCCAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGAAAGGAAGCTAACGTTGAAAAGCAATgaga                                                                                                       >  6:200994/1‑147 (MQ=255)

                                           tCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGa                                                                                                       <  6:343151/143‑1 (MQ=255)

                                           tCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGa                                                                                                       >  5:343151/1‑143 (MQ=255)

                                             aTAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATAtt                                                                                               >  7:41346/1‑149 (MQ=255)

                                              tAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTGCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTc                                                                                              <  7:302018/149‑1 (MQ=255)

                                                 ctctATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCAt                                                                                           <  2:137375/149‑1 (MQ=255)

                                                    tATCGGCAACGCGATAATTGGCAGGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACAGCTGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTGCATc                                                                                          <  4:271034/147‑1 (MQ=255)

                                                    tATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGTGTAACACCAGATGGGGAGTAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                                          <  5:306171/147‑1 (MQ=255)

                                                    tATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAgg                                                                                                                                >  6:217172/1‑109 (MQ=255)

                                                     aTCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGa                                                                                       >  7:299402/1‑149 (MQ=255)

                                                     aTCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGa                                                                                       >  5:376893/1‑149 (MQ=255)

                                                         gCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaa                                                                                    >  3:212607/1‑148 (MQ=255)

                                                         gCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaa                                                                                    >  2:13400/1‑148 (MQ=255)

                                                           aaaGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                           <  4:308192/123‑1 (MQ=255)

                                                           aaaGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                           >  3:308192/1‑123 (MQ=255)

                                                           aaaGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaaaaa                                                                                 <  4:126097/149‑1 (MQ=255)

                                                             aGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGTAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGa                                                                               <  1:279445/149‑1 (MQ=255)

                                                              gagATATTTGCCATGGAGCGTGACAGTTATGTTGAAAGGGTTAATGCACAGGAGGGGAACGCCAGAAGGGGAGGAATAATAAG‑AAAAATCTCAGCCAGCAAGCTATCGTAGAATAGCAAAGTGACAGATATTCCATCTGATAAAAAGaa                                                                              >  8:154148/3‑149 (MQ=255)

                                                                 atatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACa                                                                                                     <  2:142581/123‑1 (MQ=255)

                                                                 atatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACa                                                                                                     >  1:142581/1‑123 (MQ=255)

                                                                  tatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTAt                                                                          <  7:26862/149‑1 (MQ=255)

                                                                  tatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTAt                                                                          >  1:108230/1‑149 (MQ=255)

                                                                   atTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATg                                                                         >  8:307388/1‑149 (MQ=255)

                                                                       gCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGtt                                                                      <  7:45290/148‑1 (MQ=255)

                                                                       gCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGtt                                                                      <  2:138818/148‑1 (MQ=255)

                                                                         cATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTAt                                                                          >  1:21520/1‑142 (MQ=255)

                                                                          aTGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAg                                                                   >  2:410435/1‑148 (MQ=255)

                                                                           tGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAg                                                                   >  5:219580/1‑147 (MQ=255)

                                                                             gcgcGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGAGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAg                                                                <  3:50026/148‑1 (MQ=255)

                                                                              cgcgTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGa                                                              <  5:383407/149‑1 (MQ=255)

                                                                              cgcgTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGa                                                              <  7:307388/149‑1 (MQ=255)

                                                                                 gTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAgcg                                                           >  1:122267/1‑149 (MQ=255)

                                                                                        ttCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                    <  1:410435/149‑1 (MQ=255)

                                                                                        ttCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                    <  1:13400/149‑1 (MQ=255)

                                                                                         tCTTTTTAATTGTTTAATGGGCAGGAGTTTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGTTTTATCAGGAGCGCAttgtt                                                    <  1:450/148‑1 (MQ=255)

                                                                                           tGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAg                                                 >  5:167261/1‑149 (MQ=255)

                                                                                             ttGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGca                                               >  4:317499/1‑149 (MQ=255)

                                                                                             ttGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGca                                               >  8:363412/1‑149 (MQ=255)

                                                                                                aaTGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGcac                                              >  3:199048/1‑148 (MQ=255)

                                                                                                 aTGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAATCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAat                                           <  6:25098/149‑1 (MQ=255)

                                                                                                    ggTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatga                                         <  1:306689/148‑1 (MQ=255)

                                                                                                     gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatgatg                                       >  2:260665/1‑149 (MQ=255)

                                                                                                     gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatgatg                                       >  1:247716/1‑149 (MQ=255)

                                                                                                      ttAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGt                                      <  2:247716/149‑1 (MQ=255)

                                                                                                        aaTGGGCATGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGtt                                     <  2:122267/148‑1 (MQ=255)

                                                                                                        aaTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGtt                                     <  5:89219/148‑1 (MQ=255)

                                                                                                        aaTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGtt                                     <  3:317499/148‑1 (MQ=255)

                                                                                                         aTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTg                                    >  4:247405/1‑148 (MQ=255)

                                                                                                          tGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGa                                                  >  4:41548/1‑133 (MQ=255)

                                                                                                          tGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGa                                                  <  3:41548/133‑1 (MQ=255)

                                                                                                            ggCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaaa                                 >  8:123645/1‑148 (MQ=255)

                                                                                                                    ggTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                         >  2:266546/1‑148 (MQ=255)

                                                                                                                        acacCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAAt                     <  6:386394/148‑1 (MQ=255)

                                                                                                                         cacCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGAT‑AAAAGAATTATGGTTTAGCAGGAGCGCATTCTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTg                  >  4:2382/1‑149 (MQ=255)

                                                                                                                          acCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTg                  >  8:183547/1‑149 (MQ=255)

                                                                                                                          acCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTg                  >  4:24760/1‑149 (MQ=255)

                                                                                                                             aGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGccc               >  6:111518/1‑149 (MQ=255)

                                                                                                                                    gaggaATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTc        <  5:200994/149‑1 (MQ=255)

                                                                                                                                    gaggaATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTc        >  3:303208/1‑149 (MQ=255)

                                                                                                                                     aggaATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCg       >  7:182989/1‑149 (MQ=255)

                                                                                                                                      ggaATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCgg      <  8:182989/149‑1 (MQ=255)

                                                                                                                                       gaATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGc     >  7:232967/1‑149 (MQ=255)

                                                                                                                                          taataaG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGt  <  7:123645/149‑1 (MQ=255)

                                                                                                                                          taataaG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGt  <  4:212607/149‑1 (MQ=255)

                                                                                                                                          taataaG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGt  >  1:115454/1‑149 (MQ=255)

ATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGT  >  NC_000913/4175001‑4175288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`