Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,281,021 G→A 7.4% pseudogene (321/375 nt) agaW → pseudogene, N‑acetylgalactosamine‑specific enzyme IIC component of PTS;enzyme; Central intermediary metabolism: Amino sugars; PTS system N‑acetylgalactosameine‑specific IIC component 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,281,0210GA 7.4% 255.4 / 7.3 81pseudogene (321/375 nt)agaWpseudogene, N‑acetylgalactosamine‑specific enzyme IIC component of PTS;enzyme; Central intermediary metabolism: Amino sugars; PTS system N‑acetylgalactosameine‑specific IIC component 2
Reads supporting (aligned to +/- strand):  ref base G (33/42);  new base A (3/3);  total (36/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GTGTGGATGGGGCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATGACAGGTTTACATCACCGCGAACCGG  >  NC_000913/3280875‑3281167
                                                                                                                                                  |                                                                                                                                                  
gtgtGGATGGGGCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGa                                                                                                                                                   <  5:248327/148‑1 (MQ=255)
gtgtGGATGGGGCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGAt                                                                                                                                                  >  6:58936/1‑149 (MQ=255)
 tgtgGATGGGGCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATg                                                                                                                                                 >  2:63220/1‑149 (MQ=255)
   tgGATGGGGCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATggg                                                                                                                                               <  4:62845/149‑1 (MQ=255)
     gATGGGGCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATggg                                                                                                                                               >  1:319378/1‑147 (MQ=255)
     gATGGGGCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATggg                                                                                                                                               <  2:319378/147‑1 (MQ=255)
          ggCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGGGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAAATGGGGATTAcc                                                                                                                                        >  5:67544/1‑149 (MQ=255)
          ggCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAAATGGGGATTAcc                                                                                                                                        >  1:31257/1‑149 (MQ=255)
          ggCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAAATGGGGATTAcc                                                                                                                                        >  5:72350/1‑149 (MQ=255)
           gCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCt                                                                                                                                       <  1:294660/149‑1 (MQ=255)
               gCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCt                                                                                                                                   <  4:150693/149‑1 (MQ=255)
               gCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCt                                                                                                                                   <  3:87946/149‑1 (MQ=255)
               gCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCt                                                                                                                                   <  1:87156/149‑1 (MQ=255)
               gCCCCGCTGGCGGGCGCACAGCCGCATAACGGGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCt                                                                                                                                   <  8:168136/149‑1 (MQ=255)
                   cGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTtc                                                                                                                               <  6:22193/149‑1 (MQ=255)
                        gcgggcgCACAGCCGCCTAACGTGTTTATCGGTACTATCGTCGGGACGGCGTTTGCCATTACTACTGGCGTGAAACCCGTTGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACTGATGGGGATTACCTTCCTGTTCTCgg                                                                                                                           <  5:227152/148‑1 (MQ=255)
                        gcgggcgCACAGCCGCCTAACGTGATTATCGGTACTATAGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAACCCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGt                                                                                                                          >  5:149552/1‑149 (MQ=255)
                           ggcgCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGAt                                                                                                                       >  6:11215/1‑149 (MQ=255)
                                acaGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCg                                                                                                                  >  8:100613/1‑149 (MQ=255)
                                  aGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTc                                                                                                                                     >  5:247237/1‑128 (MQ=255)
                                  aGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTc                                                                                                                                     <  6:247237/128‑1 (MQ=255)
                                    ccgccTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTTCTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGGTCTCGGTGATGTCCGGCgg                                                                                                              >  8:131139/1‑148 (MQ=255)
                                    ccgccTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTtc                                                                                                                               <  8:133080/132‑1 (MQ=255)
                                    ccgccTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTtc                                                                                                                               >  7:133080/1‑132 (MQ=255)
                                      gccTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGa                                                                                                            <  4:25487/149‑1 (MQ=255)
                                       ccTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGAt                                                                                                           <  1:63220/149‑1 (MQ=255)
                                       ccTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGAt                                                                                                           <  4:286517/149‑1 (MQ=255)
                                       ccTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGAt                                                                                                           <  7:323831/149‑1 (MQ=255)
                                           aCGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGAATACCTTCCTGTTCACGGAGATGTCCGGCGTGATGtct                                                                                                       >  6:224837/1‑149 (MQ=255)
                                            cGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGtctc                                                                                                      >  7:134199/1‑149 (MQ=255)
                                            cGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGtctc                                                                                                      <  7:290240/149‑1 (MQ=255)
                                             gTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCg                                                                                                     >  5:135475/1‑149 (MQ=255)
                                                 ttATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGc                                                                                                 <  6:310573/149‑1 (MQ=255)
                                                      ggTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCt                                                                                            <  8:187003/149‑1 (MQ=255)
                                                       gTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGGCGCAGTACAGATTGGGATTACCTTCCTGTTCGCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTg                                                                                           >  5:250075/1‑149 (MQ=255)
                                                           tATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGcaa                                                                                       >  3:21832/1‑149 (MQ=255)
                                                           tATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGcaa                                                                                       >  2:21732/1‑149 (MQ=255)
                                                             tcgtcgGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGcaac                                                                                      <  7:100613/148‑1 (MQ=255)
                                                             tcgtcgGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTAGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGcaac                                                                                      >  2:267966/1‑148 (MQ=255)
                                                               gtcgGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACaaa                                                                                   <  4:131529/149‑1 (MQ=255)
                                                                     aCGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCcgcg                                                                             >  7:310055/1‑149 (MQ=255)
                                                                      cGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGCTGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCgcgc                                                                            >  1:261856/1‑149 (MQ=255)
                                                                           tttGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCAt                                                                       <  6:40461/149‑1 (MQ=255)
                                                                             tGCCATTACTACTTGCGGGAAACCCGATGTCGCAGTAGGGGGCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGGCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCAtt                                                                      <  6:250075/148‑1 (MQ=255)
                                                                               ccATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGa                                                                   <  5:11215/149‑1 (MQ=255)
                                                                               ccATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGa                                                                   >  2:12647/1‑149 (MQ=255)
                                                                                  ttACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGCTGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATgg                                                                >  2:143017/1‑148 (MQ=255)
                                                                                  ttACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGc                                                                <  6:135475/149‑1 (MQ=255)
                                                                                     ctactGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTg                                                              <  5:58936/148‑1 (MQ=255)
                                                                                           gCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGcc                                                         <  7:44286/147‑1 (MQ=255)
                                                                                             gTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACg                                                     >  2:135539/1‑149 (MQ=255)
                                                                                              tGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGGCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTTAGTTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACgg                                                    <  6:56540/149‑1 (MQ=255)
                                                                                                aaaCCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGcc                                                  >  8:67731/1‑149 (MQ=255)
                                                                                                  aCCCGATGGCGCAGTAGGTGTCGCCGTACCTTTAGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGGCTCGCGGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTg                                                <  5:98800/149‑1 (MQ=255)
                                                                                                   cccGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTgg                                               >  2:71720/1‑149 (MQ=255)
                                                                                                   cccGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTgg                                               >  1:235283/1‑149 (MQ=255)
                                                                                                    ccGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCGGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGCCGGCCTGGt                                              >  2:304027/1‑149 (MQ=255)
                                                                                                          tCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCTACGGCCTGGAGCAtt                                         <  1:267966/148‑1 (MQ=255)
                                                                                                          tCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAAATGGGGTTTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACTAACCCGCGGCGCATTTGATGCTGGTGCCGACGGCCTGGTGCAtt                                         <  6:67544/148‑1 (MQ=255)
                                                                                                          tCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAAATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCAtt                                         <  2:31257/148‑1 (MQ=255)
                                                                                                          tCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAAATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCAtt                                         <  6:72350/148‑1 (MQ=255)
                                                                                                          tCGCAGGAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCAtt                                         <  3:54271/148‑1 (MQ=255)
                                                                                                           cGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGCCCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGc                                       >  4:219103/1‑149 (MQ=255)
                                                                                                            gCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCt                                      <  4:21832/149‑1 (MQ=255)
                                                                                                             cAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCta                                     >  7:162914/1‑149 (MQ=255)
                                                                                                                  ggTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGAGGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACAGCCTGGTGCATTGCTATAAc                                 <  6:149552/148‑1 (MQ=255)
                                                                                                                   gtgtCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGt                                              <  6:131559/134‑1 (MQ=255)
                                                                                                                   gtgtCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGt                                              >  5:131559/1‑134 (MQ=255)
                                                                                                                      tCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTgg                                                             >  2:298743/1‑116 (MQ=255)
                                                                                                                      tCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTgg                                                             <  1:298743/116‑1 (MQ=255)
                                                                                                                      tCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGAAGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCAtt                                         >  3:104767/1‑136 (MQ=255)
                                                                                                                      tCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGAAGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCAtt                                         <  4:104767/136‑1 (MQ=255)
                                                                                                                        gCCGTACCTTTGGCTGTCGCAGTACAGAGGGGGTTTACGTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAAGCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATg                           <  7:344551/148‑1 (MQ=255)
                                                                                                                        gCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTg                                                                    >  6:30312/1‑107 (MQ=255)
                                                                                                                        gCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTg                                                                    <  5:30312/107‑1 (MQ=255)
                                                                                                                         ccGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATGa                          >  1:357341/1‑148 (MQ=255)
                                                                                                                           gTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATGACAg                       <  2:235283/149‑1 (MQ=255)
                                                                                                                              ccTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATGACAGGtt                    >  2:224409/1‑149 (MQ=255)
                                                                                                                              ccTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATGACAGGtt                    <  8:310055/149‑1 (MQ=255)
                                                                                                                               cTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATGACAGGttt                   <  1:71720/149‑1 (MQ=255)
                                                                                                                                                cAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATGACAGGTTTACATCACCGCGAACCgg  <  5:224837/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTGTGGATGGGGCTGGCCCCGCTGGCGGGCGCACAGCCGCCTAACGTGATTATCGGTACTATCGTCGGCACGGCGTTTGCCATTACTACTGGCGTGAAACCCGATGTCGCAGTAGGTGTCGCCGTACCTTTCGCTGTCGCAGTACAGATGGGGATTACCTTCCTGTTCTCGGTGATGTCCGGCGTGATGTCTCGCTGCGACCTGGCAACAAACCCGCGCCGCATTTGATGCTGGTGCCGACGGCCTGGTGCATTGCTATAACGGGATGACAGGTTTACATCACCGCGAACCGG  >  NC_000913/3280875‑3281167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: