| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,121,223 | 1 | . | A | 20.0% | 45.7 / 12.6 | 20 | intergenic (‑268/+19) | bssS/dinI | biofilm regulator/DNA damage‑inducible protein I |
| Reads supporting (aligned to +/- strand): ref base . (9/7); new base A (2/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTGTCGCGCCAATAACCGATAAATTATTCGCTGCGGCATAACGTACC > NC_000913/1121089‑1121365 | ttttAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTg > 3:70184/1‑149 (MQ=255) ttAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCa < 6:38022/144‑1 (MQ=255) ttAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCa > 5:38022/1‑144 (MQ=255) aTTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCAtatta < 4:70184/149‑1 (MQ=255) tatGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGc > 2:34714/1‑149 (MQ=255) ttcatATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCa > 8:4224/1‑135 (MQ=255) ttcatATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCa < 7:4224/135‑1 (MQ=255) tttttttAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCAc < 7:50227/148‑1 (MQ=255) cATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTg > 5:6995/1‑147 (MQ=255) aaTTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAAc > 4:99141/1‑108 (MQ=255) tAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTAtcttc < 6:6995/149‑1 (MQ=255) aTTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCt < 3:104220/100‑1 (MQ=255) aTTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCt > 4:104220/1‑100 (MQ=255) taagtaaAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTGTCGCGCCAATAACCGATa > 1:66564/1‑148 (MQ=255) aataaaaAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTGTCGCGCCAATAACCGATAAATTATTc < 1:34714/149‑1 (MQ=255) taaaaAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATcttctt < 3:98044/119‑1 (MQ=255) taaaaAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATcttctt > 4:98044/1‑119 (MQ=255) aaaaaGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTg > 2:61540/1‑119 (MQ=255) aaaaaGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTg < 1:61540/119‑1 (MQ=255) cccGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTGTCGCGCCAATAACCGATAAATTATTCGCTGCGGCATAACGTAcc > 2:104292/1‑148 (MQ=255) | TTTTAAGATTTATATGAACAATAAAACAGCATGTCATTCATATTTTTTTAGCATATTGTGCAATTATTTTGAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTGTTTATCTTCTTTTGTCGCGCCAATAACCGATAAATTATTCGCTGCGGCATAACGTACC > NC_000913/1121089‑1121365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |