Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,340	C→T	intergenic (‑257/‑30)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,340	0	C	T	100.0%	145.2 / NA	42	intergenic (‑257/‑30)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/23);  total (19/23)

ACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGG  >  NC_000913/1806196‑1806484                                                                                                                                                 |                                                                                                                                                 aCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTTCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGAc                                                                                                                                              <  5:10799/149‑1 (MQ=255)            tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGc                                                                                                                                    <  2:128492/148‑1 (MQ=255)                 tgaTTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATag                                                                                                                              >  4:138262/3‑149 (MQ=255)                    tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATaggag                                                                                                                           >  1:179008/1‑149 (MQ=255)                        aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGaaa                                                                                                                       <  7:135399/149‑1 (MQ=255)                                          tgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtat                                                                                                      <  4:154381/148‑1 (MQ=255)                                            gCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACg                                                                                                   <  2:179008/149‑1 (MQ=255)                                                aaaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                                               <  3:138262/149‑1 (MQ=255)                                                 aaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                                               >  6:121829/1‑148 (MQ=255)                                                                   tatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                                                 >  3:121784/1‑112 (MQ=255)                                                                   tatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                                                 <  4:121784/112‑1 (MQ=255)                                                                   tatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                                            <  6:656/149‑1 (MQ=255)                                                                      atTTATCTGAAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                                                   <  3:104241/107‑1 (MQ=255)                                                                       tttATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                                 >  6:77768/1‑124 (MQ=255)                                                                       tttATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                                 <  5:77768/124‑1 (MQ=255)                                                                             tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                                                                   >  5:90590/1‑148 (MQ=255)                                                                              gCAAAATTTTAAATAAAGCTCCAATAAATCATATTCTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaacaa                                                                 >  4:74548/1‑149 (MQ=255)                                                                               cAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAt                                                                >  2:177059/1‑149 (MQ=255)                                                                                aaaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAAGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAt                                                                >  2:49235/1‑148 (MQ=255)                                                                                    ttttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaa                                                                    <  7:147254/140‑1 (MQ=255)                                                                                    ttttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaa                                                                    >  8:147254/1‑140 (MQ=255)                                                                                        aaataaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                                              >  6:18663/1‑142 (MQ=255)                                                                                        aaataaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                                              <  5:18663/142‑1 (MQ=255)                                                                                           taaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                                                    <  1:49235/149‑1 (MQ=255)                                                                                           taaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                                                    <  1:177059/149‑1 (MQ=255)                                                                                              aGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTa                                                 >  2:144411/1‑149 (MQ=255)                                                                                               gCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcccc                                                          >  1:77580/1‑139 (MQ=255)                                                                                               gCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcccc                                                          <  2:77580/139‑1 (MQ=255)                                                                                               gCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTAt                                                <  6:90590/149‑1 (MQ=255)                                                                                                     aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                                          <  3:74548/149‑1 (MQ=255)                                                                                                         aTCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGaa                                      <  1:144411/149‑1 (MQ=255)                                                                                                          tCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGaa                                      >  6:18752/1‑148 (MQ=255)                                                                                                            atatTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                                                            <  7:137619/124‑1 (MQ=255)                                                                                                            atatTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                                                            >  8:137619/1‑124 (MQ=255)                                                                                                               ttGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTg                                 <  5:121829/148‑1 (MQ=255)                                                                                                                  ttAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAccc                                                           >  2:134437/1‑119 (MQ=255)                                                                                                                  ttAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAccc                                                           <  1:134437/119‑1 (MQ=255)                                                                                                                  ttAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCt                             >  4:78952/1‑149 (MQ=255)                                                                                                                                    gCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTc            <  3:38015/148‑1 (MQ=255)                                                                                                                                    gCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTc            >  7:53833/1‑148 (MQ=255)                                                                                                                                    gCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTAGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTc            >  4:38015/1‑148 (MQ=255)                                                                                                                                             gTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCgggg  <  5:18752/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 ACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGG  >  NC_000913/1806196‑1806484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑