Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,596,873 Δ1 bp 5.1% intergenic (‑136/+32) ypfJ ← / ← purC putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,596,8730C. 5.1% 333.3 / 10.0 98intergenic (‑136/+32)ypfJ/purCputative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (49/44);  new base . (3/2);  total (52/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

AACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACA  >  NC_000913/2596731‑2597020
                                                                                                                                              |                                                                                                                                                    
aaCGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTg                                                                                                                                               >  8:58075/1‑149 (MQ=255)
  cGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCa                                                                                                                                             <  2:360356/149‑1 (MQ=255)
    cATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACg                                                                                                                                           >  5:191117/1‑149 (MQ=255)
     aTAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACgg                                                                                                                                          >  8:51617/1‑149 (MQ=255)
     aTAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGCTTGTCTTGCCGTGCCACCTGCACgg                                                                                                                                          >  1:210373/1‑149 (MQ=255)
         aTACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                                                                         >  5:61033/1‑146 (MQ=255)
            ccTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgat                                                                                                                                    >  8:161736/1‑148 (MQ=255)
             cTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGc                                                                                                                                                  <  1:273875/134‑1 (MQ=255)
             cTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGc                                                                                                                                                  >  2:273875/1‑134 (MQ=255)
             cTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGGTGGCTTGCCGTGCCAGCTGCACGGCAAgatga                                                                                                                                  <  3:169504/149‑1 (MQ=255)
             cTCAACAATATATTCATTATAGAAATGATCGGAGGTGGTTCAGAGGAAGATTACCACACGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATAAATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatga                                                                                                                                  <  3:310392/149‑1 (MQ=255)
              tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                                                                       >  8:309068/1‑143 (MQ=255)
              tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                                                                       >  8:314361/1‑143 (MQ=255)
              tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                                                                       <  7:309068/143‑1 (MQ=255)
              tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                                                                       <  7:314361/143‑1 (MQ=255)
                  caaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGaa                                                                                                                              >  7:300521/1‑148 (MQ=255)
                   aaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATTGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGATTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACa                                                                                                                            <  3:60390/149‑1 (MQ=255)
                         ttCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaaaa                                                                                                                      >  3:175292/1‑149 (MQ=255)
                            aTTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGATGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAt                                                                                                                     >  1:177874/1‑147 (MQ=255)
                            aTTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCa                                                                                                                   <  3:119645/149‑1 (MQ=255)
                            aTTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCa                                                                                                                   <  6:61033/149‑1 (MQ=255)
                             ttATAGAAATGATCGTAGGTTGTTCAGACGAATATTACCACAGTAAAGCGGGATTAAATGGCGGAGGAATGATCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAAGCTGCACGGCAAGATGATGAACAGAAAAATAAg                                                                                                                  >  3:23781/1‑149 (MQ=255)
                                taGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGTAGGAATGAGCGTTCAATGCATATAATCCGAGAGCGGTTGTCTTTCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTc                                                                                                                >  7:331701/1‑148 (MQ=255)
                                  gAACTGATCGTTGGTGGTTCAGAGGAAGAGTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAg                                                                                                             <  2:210373/149‑1 (MQ=255)
                                   aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAg                                                                                                                                                   >  5:32875/1‑111 (MQ=255)
                                   aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAg                                                                                                                                                   <  6:32875/111‑1 (MQ=255)
                                   aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGc                                                                                                            >  5:143688/1‑149 (MQ=255)
                                     aTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTc                                                                                                                <  3:176834/143‑1 (MQ=255)
                                     aTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTc                                                                                                                >  4:176834/1‑143 (MQ=255)
                                     aTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTg                                                                                                          <  1:273074/149‑1 (MQ=255)
                                       gATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa                                                                                                        >  6:145208/1‑149 (MQ=255)
                                        aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTac                                                                                                       >  8:21226/1‑149 (MQ=255)
                                        aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTac                                                                                                       <  2:6208/149‑1 (MQ=255)
                                        aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa                                                                                                        <  6:143688/148‑1 (MQ=255)
                                         tCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTaca                                                                                                      >  6:223141/1‑149 (MQ=255)
                                            tAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATATCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAcc                                                                                                    >  5:157110/1‑148 (MQ=255)
                                             aGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAcc                                                                                                    >  6:104736/1‑147 (MQ=255)
                                              ggtggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGTCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAg                                                                                                 >  6:101622/1‑149 (MQ=255)
                                              ggtggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAg                                                                                                 <  7:21226/149‑1 (MQ=255)
                                                  gtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTAGGCTTGCCGTGCCAGCTTCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAggcg                                                                                              <  1:262092/148‑1 (MQ=255)
                                                      agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg                                                                                          <  5:104736/148‑1 (MQ=255)
                                                      agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcggg                                                                                         >  8:321227/1‑149 (MQ=255)
                                                      agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAAAAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg                                                                                          <  7:51617/148‑1 (MQ=255)
                                                      agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCAGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcggg                                                                                         >  3:107693/1‑149 (MQ=255)
                                                        agGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcgggcg                                                                                       >  8:322671/1‑149 (MQ=255)
                                                         ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAccc                                                                                                   >  8:159812/1‑136 (MQ=255)
                                                         ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAccc                                                                                                   <  7:159812/136‑1 (MQ=255)
                                                            aGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                                                                   <  7:161736/149‑1 (MQ=255)
                                                                  ccACAGGAAAGCGGGATGAATTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGAAAGATGATGTCCAGAAAAATCAGTCCCGCGGTAATCCCAGTCGGCGGGCGACGCCTTCa                                                                              >  2:68668/1‑148 (MQ=255)
                                                                       ggAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCTGGCGACGGCTTCATAGGCt                                                                        <  8:84640/149‑1 (MQ=255)
                                                                           aGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGa                                                                    <  5:85196/149‑1 (MQ=255)
                                                                              gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCa                                                                 <  7:158833/149‑1 (MQ=255)
                                                                              gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCa                                                                 <  1:132564/149‑1 (MQ=255)
                                                                              gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCa                                                                 <  5:16435/149‑1 (MQ=255)
                                                                               ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAg                                                                <  5:223141/149‑1 (MQ=255)
                                                                               ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAg                                                                <  8:48297/149‑1 (MQ=255)
                                                                                gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCCCGGCAAGATGATGAACCGAAAACTCAGTCCAGCTGTACACCCAGGCGGCGCGCGACGGCTTCATAGGCTTCGATCAgg                                                               >  5:296958/1‑149 (MQ=255)
                                                                                gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg                                                               >  1:256953/1‑149 (MQ=255)
                                                                                 aTGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACCCCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGc                                                              >  8:4062/1‑149 (MQ=255)
                                                                                       tGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGa                                                        <  8:300521/149‑1 (MQ=255)
                                                                                         gCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGTCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAg                                                       <  1:68668/148‑1 (MQ=255)
                                                                                           ggaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGct                                                    >  1:109011/1‑149 (MQ=255)
                                                                                            gaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGctc                                                   >  3:110107/1‑149 (MQ=255)
                                                                                             aggaATGAGCGGTCACGGAATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCCCAGCAAGATGATGAACAGAAGAATGAGTACAGCTGTACACCCAGGCGCCGGGCGACGGCTTCATAGGCTGCGATCAGGCCACCGAGGctct                                                  >  6:118089/1‑149 (MQ=255)
                                                                                               gaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCCATCAGGCCACCGAGGCTCTgg                                                >  2:97163/1‑149 (MQ=255)
                                                                                                 aTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg                                              >  8:264411/1‑149 (MQ=255)
                                                                                                   gAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCgg                                             >  5:103467/1‑148 (MQ=255)
                                                                                                       gTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCCGGCGGCGGGCGCCGGCTTCATAGGCATCGATCAGGCCACCGCGGCTCTGGCGGCAACg                                        >  8:152312/1‑149 (MQ=255)
                                                                                                        ttCAATCCATATATTCCGAGATCGGGTGGCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAACATCAGTCCACCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGCTCAGGCCACCGAGGCTCTGGCGGAAACgg                                       <  3:34102/149‑1 (MQ=255)
                                                                                                           aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTc                                     <  4:175292/148‑1 (MQ=255)
                                                                                                           aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGGGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCt                                    <  5:205458/149‑1 (MQ=255)
                                                                                                            aTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTCCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCtt                                   >  1:277411/1‑149 (MQ=255)
                                                                                                             ttcATATATTACGAGTGCGGTTGGCTTGCAGTGCCTGCTGCAAGGAAAGATGATTTACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGGTTACTAGGCTTCGATCAGGCCACGGATGATCTGGCGGAAACGGTCttt                                  <  4:23781/147‑1 (MQ=255)
                                                                                                             tGCATATATTCCGAGAGCTGTTGTCTTGCCGTGCGAGCTGCACGGCAAGATGATGAAGAGAAAAATCAGTCCAGGTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCttt                                  <  4:107693/149‑1 (MQ=255)
                                                                                                             tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCttt                                  <  7:321227/149‑1 (MQ=255)
                                                                                                                atatatTCCGAGAGCGGTTGTCTTGCCGTGCCGGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTc                               >  8:225034/1‑149 (MQ=255)
                                                                                                                  atatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCttt                                  >  4:82251/1‑144 (MQ=255)
                                                                                                                  atatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCttt                                  <  3:82251/144‑1 (MQ=255)
                                                                                                                    atTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCGCTGGCGGAAACGGTCt                                    <  7:279944/140‑1 (MQ=255)
                                                                                                                    atTCCGAGAGCGGTTCTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGACGCTCGGGCGGAAACGGACt                                    >  8:279944/1‑140 (MQ=255)
                                                                                                                     ttCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCAttt                          >  7:153698/1‑149 (MQ=255)
                                                                                                                        cGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtct                       >  6:263216/1‑149 (MQ=255)
                                                                                                                        cGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtc                        <  6:191117/148‑1 (MQ=255)
                                                                                                                          agagCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGCTGAACAGAAAAATCAGTCCCGGTGTACCCCCAGGCGGCGGGCGCCGGCTTCATAGGCTTCTCTCAGGCCACCGCGGCTCTGGCGGCAACGGGCTTTGGCCATTTtctc                      >  1:300188/1‑148 (MQ=255)
                                                                                                                          agagCGCTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGCCGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtctc                      >  8:178494/1‑148 (MQ=255)
                                                                                                                           gagCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCTATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCa                    <  5:145208/149‑1 (MQ=255)
                                                                                                                           gaccGGGTGTCTTGCCGTGCCAGCTGCACTGCAAGATGATGAACAGAAAAATCAGTCCCGCTGAACACCCATTCGTCGGGCGACGGCTTCATACGCTTCTATCAGGCCTACGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCa                    <  2:98179/146‑1 (MQ=255)
                                                                                                                            agCGGTTGTCTTGCCGTGCCAGCAGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGCCGGCTTCATAGGCTTCGCTAAGGCCACCGCGGCTCTGGCGGAAACGGTCATTGTCCATTTTCTCCAg                   >  8:235439/1‑149 (MQ=255)
                                                                                                                               catcatCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGt                >  4:202217/6‑149 (MQ=255)
                                                                                                                                gTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGtt               >  3:260816/1‑149 (MQ=255)
                                                                                                                                 ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTcc                     >  3:336832/1‑142 (MQ=255)
                                                                                                                                 ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTcc                     <  4:336832/142‑1 (MQ=255)
                                                                                                                                    tCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCGTTGTCCATTTTCTCCCGCGTTTCtt           >  4:191231/1‑149 (MQ=255)
                                                                                                                                        gCCGTGCCAGCTGCACGGCAAGATGATGAAGAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGAGGGCTTCATAGGCTTCTATCAGGCCACCGAGGCTCTGGCTGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCttt          <  6:157110/146‑1 (MQ=255)
                                                                                                                                        gCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGt        <  7:152312/148‑1 (MQ=255)
                                                                                                                                         ccGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGGCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc       <  7:322671/148‑1 (MQ=255)
                                                                                                                                           gTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCca    <  4:260816/149‑1 (MQ=255)
                                                                                                                                            tGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCca    >  4:264154/1‑148 (MQ=255)
                                                                                                                                              ccAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCcaca  <  4:110107/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
AACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACA  >  NC_000913/2596731‑2597020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: