New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3948426 =125 (1.520)4 (0.050) 4/246 NT 3.4% intergenic (+2/+23) yifE/yifB UPF0438 family protein/magnesium chelatase family protein and putative transcriptional regulator
?NC_000913 3948449 = 108 (1.410)coding (1521/1521 nt) yifB magnesium chelatase family protein and putative transcriptional regulator
Rejected: Frequency below/above cutoff threshold.

TTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3948580‑3948426
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccctttttTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCAC  >  NC_000913/3948449‑3948594
                                                                                                                                                                                                                                                                                                             
TTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCC                                                                                                                                                          >  2:289479/1‑149
  ATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCC                                                                                                                                                         <  7:332930/148‑1
   TTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTT                                                                                                                                                       >  1:332127/1‑149
   TTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTT                                                                                                                                                       >  3:200431/1‑149
   TTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTT                                                                                                                                                       >  4:162389/1‑149
      AAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTTTT                                                                                                                                                     >  2:101576/1‑148
      AAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTTTT                                                                                                                                                     <  4:130308/148‑1
      AAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTTTT                                                                                                                                                     >  6:207111/1‑148
             CACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGC                                                                                                                                                           <  6:45924/135‑1
                                                                                                             ATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGAC                                                                       <  8:331738/123‑1
                                                                                                             ATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGAC                                                                       >  7:331738/1‑123
                                                                                                                               CATAAAAAAAGGGCATTTCGCCCTTTTTTTATGTCAGTAGTTTATGAAGATGGATGAGCAAACGGTCAATCGCTCGATGGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGGCAGACTGATCATTATCAGCAATGGTTCtggc                            >  6:142644/1‑144
                                                                                                                                 TAAAAAAAGGGCATTTCGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAAC                         >  4:178171/1‑149
                                                                                                                                                   CCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCA       <  1:317154/149‑1
                                                                                                                                                      TTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGG     >  1:271421/1‑148
                                                                                                                                                      TTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGG     >  8:354197/1‑148
                                                                                                                                                      TTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTGCAATAACCGCTGCCAGG     >  2:133441/1‑148
                                                                                                                                                         TTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCAC  <  4:29512/148‑1
                                                                                                                                                                                                                                                                                                             
TTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATAAAAAAAGGGCATTTCGCCCTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3948580‑3948426
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccctttttTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCAC  >  NC_000913/3948449‑3948594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.