Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 128,803 G→A 5.9% D298N (GAC→AAC)  lpd → dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913128,8030GA 5.9% 208.9 / 2.1 68D298N (GAC→AAC) lpddihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (34/29);  new base A (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

TGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACACTACTTCGATCCGA  >  NC_000913/128666‑128944
                                                                                                                                         |                                                                                                                                             
tGGAAGGCAAAAAAGCACCCGCTGCACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGGGTGCCGAACGGTAAAAACCTCGCCGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACACACAGCTg                                                                                                                                    >  6:97230/1‑149 (MQ=255)
  gAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGc                                                                                                                                   <  1:22512/148‑1 (MQ=255)
  gAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGCTGACAAACAGCTGCg                                                                                                                                  >  4:341310/1‑149 (MQ=255)
    aGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTa                                                                                                                                >  5:198011/1‑149 (MQ=255)
        aaaaaaGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCaa                                                                                                                            >  2:366043/1‑149 (MQ=255)
            aaGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTa                                                                                                                        <  1:15276/149‑1 (MQ=255)
             aGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTAc                                                                                                                       <  4:182428/149‑1 (MQ=255)
                aCCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGAGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGc                                                                                                                    <  7:268230/149‑1 (MQ=255)
                 cccGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGca                                                                                                                   >  5:334838/1‑149 (MQ=255)
                   cGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGcaca                                                                                                                 >  6:58378/1‑149 (MQ=255)
                      tGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCt                                                                                                              >  3:280449/1‑149 (MQ=255)
                        aaCCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCttt                                                                                                            >  4:161452/1‑149 (MQ=255)
                          ccGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGc                                                                                                          <  4:188205/149‑1 (MQ=255)
                           cGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCa                                                                                                                             <  4:286130/129‑1 (MQ=255)
                           cGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGc                                                                                                          >  8:362738/1‑148 (MQ=255)
                            gCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTa                                                                                                        <  1:366043/149‑1 (MQ=255)
                             cAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTa                                                                                                                        <  4:328444/132‑1 (MQ=255)
                             cAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTa                                                                                                                        >  3:328444/1‑132 (MQ=255)
                                    acgacgCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGa                                                                                                 <  1:71706/148‑1 (MQ=255)
                                           cGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATAtcgtcg                                                                                         <  3:55968/149‑1 (MQ=255)
                                            gTGCTTGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCgg                                                                                        <  1:285197/149‑1 (MQ=255)
                                                  gTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAAcc                                                                                  >  4:331454/1‑149 (MQ=255)
                                                     gCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGAt                                                                               >  5:320112/1‑149 (MQ=255)
                                                      cGATTGGTCGGGTGCCGAACGGTAAAAACCTCCACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATg                                                                              <  2:149794/149‑1 (MQ=255)
                                                            gTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAGCCTCTGCTGGca                                                                        >  2:273968/1‑149 (MQ=255)
                                                                   gCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGCCGACCGTGGTTTCATCCGCGTTAACAAACAGCTGCGTACCAACGTACCGCACATCGTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAg                                                                 >  5:18885/1‑149 (MQ=255)
                                                                   gCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGCCCGTGGTTTCATCCGCGTTAACAAACAGCTGCGTACCACCGTACCGCACATCTTTGCTATCGGCGATAGCGTCGGTCCACCGATGCTGGCACACAAAg                                                                 >  5:24119/1‑149 (MQ=255)
                                                                    ccGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAg                                                                 <  7:309888/148‑1 (MQ=255)
                                                                    ccGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAg                                                                 <  5:58378/148‑1 (MQ=255)
                                                                    ccGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCAGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAgg                                                                >  5:90046/1‑149 (MQ=255)
                                                                     cGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGgt                                                               >  3:146935/1‑149 (MQ=255)
                                                                        aCGGTAAAACCCTCGACGCAGGCAAAGCCGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTAACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTAGCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGtt                                                            <  6:24119/149‑1 (MQ=255)
                                                                        aCGGTAAAAACCTCGACGCCGGCAAAGCAGGAGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTAACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGtt                                                            <  6:18885/149‑1 (MQ=255)
                                                                         cGGTAAAAACCTCGACTCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTc                                                           <  7:362738/149‑1 (MQ=255)
                                                                          ggTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTCCCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCa                                                          >  5:319469/1‑149 (MQ=255)
                                                                          ggTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCa                                                          <  4:64415/149‑1 (MQ=255)
                                                                           gTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGCTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCAc                                                         <  3:341310/149‑1 (MQ=255)
                                                                             aaaaaCCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACg                                                        >  5:242799/1‑148 (MQ=255)
                                                                              aaaaCCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAAc                                                                                                                                         >  6:216018/1‑66 (MQ=255)
                                                                              aaaaCCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAAc                                                                                                                                         <  5:216018/66‑1 (MQ=255)
                                                                               aatccTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCATCGTACCGCACATCttt                                                                                                            <  2:249525/91‑1 (MQ=255)
                                                                               aaaaCTCGACGCAGGCAAAGCAGGATTGGAAGTTGACGACAGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCttt                                                                                                            >  1:249525/1‑94 (MQ=255)
                                                                               aaaCCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCttt                                                                                                            >  1:355688/1‑94 (MQ=255)
                                                                               aaaCCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCttt                                                                                                            <  2:355688/94‑1 (MQ=255)
                                                                                aaCCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAg                                                     <  5:236954/148‑1 (MQ=255)
                                                                                aaCCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAg                                                     >  6:236954/1‑148 (MQ=255)
                                                                                  ccTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGt                                                   >  5:194895/1‑148 (MQ=255)
                                                                                   cTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGatat                                                                                              <  8:22727/104‑1 (MQ=255)
                                                                                   cTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGatat                                                                                              >  7:22727/1‑104 (MQ=255)
                                                                                   cTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCa                                                 >  1:266724/1‑149 (MQ=255)
                                                                                       aCGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGtt                                             >  2:43812/1‑149 (MQ=255)
                                                                                             gCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCAc                                                <  1:153914/140‑1 (MQ=255)
                                                                                             gCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCAc                                                >  2:153914/1‑140 (MQ=255)
                                                                                               aaaGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACCAACAGCTGCGTACCAACGTCCCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGCTGCTGGCCCACAACGGTGTTCACGAAGGTCACGTGGCCGCTg                                      >  5:117195/1‑148 (MQ=255)
                                                                                                 aGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAg                                   >  5:212945/1‑149 (MQ=255)
                                                                                                  gaaGGCTTGGAAGTTGACGACCGTCGTTTCAACCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCAATCGTCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTc                                                           >  2:64533/3‑124 (MQ=255)
                                                                                                  gCAGGCGTTGAATTTGACGACCGTGGTTTCATCCGAGTTTACAATCAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAAGAGTTGCTGGCAGACATTGGTGTTc                                                           <  1:64533/124‑1 (MQ=255)
                                                                                                        gTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGc                            <  3:161452/149‑1 (MQ=255)
                                                                                                           gAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCGGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCgg                         <  1:273968/149‑1 (MQ=255)
                                                                                                                 gacgacCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGa                                     >  7:301360/1‑131 (MQ=255)
                                                                                                                 gacgacCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGa                                     <  8:301360/131‑1 (MQ=255)
                                                                                                                    gacCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACTTTGCCGCTGAAGTTATCGCCGGTAAGAAaca                >  4:250844/1‑149 (MQ=255)
                                                                                                                     acCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAaca                >  8:56759/1‑148 (MQ=255)
                                                                                                                       cGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACacta             >  6:168684/1‑149 (MQ=255)
                                                                                                                       cGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACacta             <  6:242799/149‑1 (MQ=255)
                                                                                                                           gTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACACTACtt          <  4:146935/148‑1 (MQ=255)
                                                                                                                                   cgcgTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACACTACTTCGATCCGa  <  6:334838/148‑1 (MQ=255)
                                                                                                                                    gcgTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACACTACTTCGATCCGa  >  2:250564/1‑147 (MQ=255)
                                                                                                                                         |                                                                                                                                             
TGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTGGCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACACTACTTCGATCCGA  >  NC_000913/128666‑128944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: