Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,183,782 C→T 5.1% A221A (GCG→GCA potB ← spermidine/putrescine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,183,7820CT 5.1% 257.1 / 2.3 78A221A (GCG→GCApotBspermidine/putrescine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (38/36);  new base T (2/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

CTTATTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCC  >  NC_000913/1183635‑1183926
                                                                                                                                                   |                                                                                                                                                
cttATTCAGCAAACGCGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAAGGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTc                                                                                                                                                  <  7:375911/148‑1 (MQ=255)
cttATTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTTAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTc                                                                                                                                                  <  1:178396/148‑1 (MQ=255)
cttATTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTc                                                                                                                                                  <  6:338336/148‑1 (MQ=255)
cttATTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTc                                                                                                                                                  <  3:271424/148‑1 (MQ=255)
 ttATTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCg                                                                                                                                                 >  4:222525/1‑148 (MQ=255)
   aTTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAACCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCAc                                                                                                                                              >  5:66348/1‑149 (MQ=255)
   aTTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCa                                                                                                                                               <  3:16008/148‑1 (MQ=255)
       aGCAAACGAGAAGAGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGcc                                                                                                                                          >  7:245286/1‑149 (MQ=255)
          aaaCGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCGGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCAt                                                                                                                                       >  3:232797/1‑149 (MQ=255)
            aCGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCAt                                                                                                                                       >  4:207519/1‑147 (MQ=255)
                 aaGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCg                                                                                                                                <  5:67904/149‑1 (MQ=255)
                       gcCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACa                                                                                                                          <  2:226404/149‑1 (MQ=255)
                       gcCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACa                                                                                                                          <  2:231756/149‑1 (MQ=255)
                        ccAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACAt                                                                                                                         <  6:66348/149‑1 (MQ=255)
                         cAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACa                                                                                                                          >  6:118586/1‑147 (MQ=255)
                                ccAGCAACATCAGGCCCATTCCGATAGGCAGCGTAATGCTGGTAGCGGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAgg                                                                                                                 <  2:138211/149‑1 (MQ=255)
                                ccAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAgg                                                                                                                 <  3:222525/149‑1 (MQ=255)
                                  aGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGcc                                                                                                               >  3:156330/1‑149 (MQ=255)
                                         tCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCt                                                                                                        <  5:335807/149‑1 (MQ=255)
                                             gCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGgca                                                                                                    <  3:156888/149‑1 (MQ=255)
                                               ccATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGgcag                                                                                                   >  2:269990/1‑148 (MQ=255)
                                                  ttACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATc                                                                                               >  7:172382/1‑149 (MQ=255)
                                                     cGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCAc                                                                                             <  3:323532/148‑1 (MQ=255)
                                                      gATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACa                                                                                                                          <  3:159392/118‑1 (MQ=255)
                                                      gATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACa                                                                                                                          >  4:159392/1‑118 (MQ=255)
                                                       aTAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACcag                                                                                          <  6:145685/149‑1 (MQ=255)
                                                          gTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGaa                                                                                                                     <  2:81866/119‑1 (MQ=255)
                                                          gTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGaa                                                                                                                     >  1:81866/1‑119 (MQ=255)
                                                          gTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACcagca                                                                                        <  3:207519/148‑1 (MQ=255)
                                                          gTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACcagca                                                                                        <  3:56584/148‑1 (MQ=255)
                                                           tCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGa                                                                                      >  2:55798/1‑149 (MQ=255)
                                                           acAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGGCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCa                                                                                                                                        <  4:47529/98‑1 (MQ=255)
                                                             aGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGAc                                                                                     >  7:180230/1‑148 (MQ=255)
                                                             aGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATAAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGAc                                                                                     >  4:185999/1‑148 (MQ=255)
                                                                      cTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCaa                                                                            <  8:245286/148‑1 (MQ=255)
                                                                         gTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCaataa                                                                         <  2:353025/148‑1 (MQ=255)
                                                                         gTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCaata                                                                          <  4:156330/147‑1 (MQ=255)
                                                                            gCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTcc                                                                     >  7:224660/1‑149 (MQ=255)
                                                                            gCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTcc                                                                     <  8:172382/149‑1 (MQ=255)
                                                                             cTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTccc                                                                    >  4:23229/1‑149 (MQ=255)
                                                                                cACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCg                                                                   <  1:35412/147‑1 (MQ=255)
                                                                                 aCCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCa                                                                >  8:241196/1‑149 (MQ=255)
                                                                                     aaCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCAc                                                                                             >  3:55455/1‑116 (MQ=255)
                                                                                     aaCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCAc                                                                                             >  3:50719/1‑116 (MQ=255)
                                                                                     aaCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCAc                                                                                             <  4:55455/116‑1 (MQ=255)
                                                                                     aaCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCAc                                                                                             <  4:50719/116‑1 (MQ=255)
                                                                                          ccAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTg                                                       <  1:307901/149‑1 (MQ=255)
                                                                                           cAGTCACGAATATTAAGGAACTGGCCCTTGATGACGTTTCCGATCAGCTGGGTTTTCGCACGGCCGGTCAGGTCGGGTACCTAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACTTCCGGCAATAATTCCCGGCATGGGCAGTg                                                       >  2:172780/1‑148 (MQ=255)
                                                                                             gTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGaa                                                    <  5:118586/149‑1 (MQ=255)
                                                                                                aCGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGCCATCCGGCAATAATTCCCGGCATCGTCAGTGGaataa                                                 >  8:298906/1‑149 (MQ=255)
                                                                                                aCGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGaataa                                                 >  8:230896/1‑149 (MQ=255)
                                                                                                  gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTTGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCgg                                                                               >  5:264805/1‑117 (MQ=255)
                                                                                                  gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTTGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCgg                                                                               >  5:269843/1‑117 (MQ=255)
                                                                                                  gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTTGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCgg                                                                               <  6:264805/117‑1 (MQ=255)
                                                                                                  gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTTGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCgg                                                                               <  6:269843/117‑1 (MQ=255)
                                                                                                  gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATg                                               <  3:108770/149‑1 (MQ=255)
                                                                                                  gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGCTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAAATCCCGGCATCGTCAGTGGaataat                                                >  2:301186/1‑148 (MQ=255)
                                                                                                   aaTATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGa                                              <  4:184199/149‑1 (MQ=255)
                                                                                                       ttAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACg                                          >  3:61538/1‑149 (MQ=255)
                                                                                                        tAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACg                                          >  3:64921/1‑148 (MQ=255)
                                                                                                                 ggACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTc                                >  8:189063/1‑149 (MQ=255)
                                                                                                                  gACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGt                                 <  3:298174/147‑1 (MQ=255)
                                                                                                                   aCCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTg                              <  8:180230/149‑1 (MQ=255)
                                                                                                                    ccTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTg                              >  2:179482/1‑148 (MQ=255)
                                                                                                                        gatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAAc                          <  3:23229/148‑1 (MQ=255)
                                                                                                                        gatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACt                         >  8:356149/1‑149 (MQ=255)
                                                                                                                        gatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACt                         >  3:34689/1‑149 (MQ=255)
                                                                                                                        gatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGGCAGTGGAATAATGATACGGATAAAAGTCTGTAACt                         >  4:28714/1‑149 (MQ=255)
                                                                                                                            aCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCt                     <  7:379757/149‑1 (MQ=255)
                                                                                                                                  ccGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCAc                <  1:269990/148‑1 (MQ=255)
                                                                                                                                    gATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCga             <  4:34689/149‑1 (MQ=255)
                                                                                                                                     aTCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCAcc               >  2:192217/1‑146 (MQ=255)
                                                                                                                                       cagcagGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGTATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCAcc               >  2:276505/1‑144 (MQ=255)
                                                                                                                                        agcagGTTTGTCTCACCGCACATCAGGTCGGATACATAGAACCGGCCCATCGCTGGCAGCATCAGCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGGGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATc         <  1:103037/149‑1 (MQ=255)
                                                                                                                                         gcagGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcg        >  4:230900/1‑149 (MQ=255)
                                                                                                                                          cagGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCGTCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcgc       >  2:352785/1‑149 (MQ=255)
                                                                                                                                          cagGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGt                                 <  8:357423/123‑1 (MQ=255)
                                                                                                                                          cagGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCCGCAATAAATCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcgc       >  8:240210/1‑149 (MQ=255)
                                                                                                                                            ggTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGTATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcgcgc     <  1:276505/149‑1 (MQ=255)
                                                                                                                                             gTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcgcgcg    >  7:110334/1‑149 (MQ=255)
                                                                                                                                             gTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATcgcgcg    >  8:385352/1‑149 (MQ=255)
                                                                                                                                               ttttCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGcgcc  >  3:118120/1‑149 (MQ=255)
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CTTATTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGGAATAATGATACGGATAAAAGTCTGTAACTTGCTGGCACCGAGATCGCGCGCC  >  NC_000913/1183635‑1183926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: