Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,395,089 G→T 6.0% F278L (TTC→TTA) ‡ ynaI ← mechanosensitive channel protein, very small conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,395,0890GT 6.0% 223.3 / 2.6 67F278L (TTC→TTA) ‡ynaImechanosensitive channel protein, very small conductance
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base T (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

GGAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTG  >  NC_000913/1394944‑1395237
                                                                                                                                                 |                                                                                                                                                    
ggAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGaaa                                                                                                                                                   <  7:286021/149‑1 (MQ=255)
ggAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGaaa                                                                                                                                                   <  7:257960/149‑1 (MQ=255)
 gAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAAt                                                                                                                                                  <  2:155900/149‑1 (MQ=255)
     gCAAAGTCCGTGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAAc                                                                                                                                              <  5:184754/149‑1 (MQ=255)
     gCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAAc                                                                                                                                              >  3:300870/1‑149 (MQ=255)
      cAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAAcc                                                                                                                                             <  6:42437/149‑1 (MQ=255)
       aaaGTACGCGCCGTGTGAATTTTCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCa                                                                                                                                            <  1:241366/149‑1 (MQ=255)
          gTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGt                                                                                                                                          >  6:14845/1‑148 (MQ=255)
             cgcgCCGTGTGACTGTACAATATCGATAATCTTCAAATAACCGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGGTTTGGTAAAGCAATAAACAATAAGATTCAATGAAGAGGACGCAAAATGGGTGAAATAAACCCGTACgg                                                                                                                                      >  5:40090/1‑149 (MQ=255)
              gcgcCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGt                                                                                                                                     >  7:343672/1‑149 (MQ=255)
              gcgcCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGt                                                                                                                                     <  8:147930/149‑1 (MQ=255)
               cgcCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAACCTGGTTGAAATAAACCAGTAAGGtt                                                                                                                                    <  5:217078/149‑1 (MQ=255)
                  cGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGt                                                                                                                                 <  7:344673/149‑1 (MQ=255)
                           gTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTc                                                                                                                         >  3:148070/1‑148 (MQ=255)
                              cAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCt                                                                                                                             >  6:299667/1‑141 (MQ=255)
                              cAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCt                                                                                                                             <  5:299667/141‑1 (MQ=255)
                               aaTATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGaa                                                                                                                                                    <  8:218793/117‑1 (MQ=255)
                               aaTATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGaa                                                                                                                                                    >  7:218793/1‑117 (MQ=255)
                                atatCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGc                                                                                                                   >  7:153805/1‑149 (MQ=255)
                                 tatCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGcc                                                                                                                  <  5:327646/149‑1 (MQ=255)
                                        aaTCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATaaa                                                                                                                                               >  2:336840/1‑113 (MQ=255)
                                        aaTCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATaaa                                                                                                                                               <  1:336840/113‑1 (MQ=255)
                                            ttCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTATAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGAttttt                                                                                                       >  6:297422/1‑149 (MQ=255)
                                             tCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGAttttt                                                                                                       >  6:107794/1‑148 (MQ=255)
                                              cAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTc                                                                                                      <  8:95840/148‑1 (MQ=255)
                                               aaaTAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCa                                                                                                     <  8:343672/148‑1 (MQ=255)
                                                          ttGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTa                                                                                         >  2:22073/1‑149 (MQ=255)
                                                            gCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAAACATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTa                                                                                         <  6:50118/147‑1 (MQ=255)
                                                            gCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTGTTGGTAAAGCAATACACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGGCGATGGCCGGGTGATTTTTCAGCATCTCACGTa                                                                                         <  4:269383/147‑1 (MQ=255)
                                                             cTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACa                                                                                       <  5:14845/148‑1 (MQ=255)
                                                              tgtgCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGc                                                                                     >  8:208137/1‑149 (MQ=255)
                                                                tgCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCtt                                                                                   <  1:137226/149‑1 (MQ=255)
                                                                  cagcaAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTc                                                                                  <  7:15168/148‑1 (MQ=255)
                                                                     caAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTAAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGAc                                                                               >  8:130935/1‑148 (MQ=255)
                                                                       aGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAAt                                                                            <  5:152004/149‑1 (MQ=255)
                                                                            ttCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAAt                                                                            <  2:27838/144‑1 (MQ=255)
                                                                            ttCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAAt                                                                            >  1:27838/1‑144 (MQ=255)
                                                                                   cATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACttt                                                                <  7:208137/149‑1 (MQ=255)
                                                                                     tACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTg                                                              >  8:187643/1‑149 (MQ=255)
                                                                                     tACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAAACAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTg                                                              >  8:323549/1‑149 (MQ=255)
                                                                                       ccGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGcc                                                            <  3:315973/149‑1 (MQ=255)
                                                                                       ccGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGcc                                                            <  8:225253/149‑1 (MQ=255)
                                                                                            gTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCAt                                                        <  1:141860/148‑1 (MQ=255)
                                                                                             ttttGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAg                                                                                                    <  5:182250/103‑1 (MQ=255)
                                                                                             ttttGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAg                                                                                                    >  6:182250/1‑103 (MQ=255)
                                                                                             ttttGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGAGTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATcc                                                      <  4:311786/149‑1 (MQ=255)
                                                                                             ttttGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGGGATTGTTCAGCATCTGAGGTGCAGCTTCGACAATAACGGCCACATTTGCCGCATcc                                                      >  6:278330/1‑149 (MQ=255)
                                                                                                              aCCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACCGCTTCGACAATAACGCCCACTTTTGCCGCCTCCTCCTAACGTAAACCAAt                                     >  2:220589/1‑149 (MQ=255)
                                                                                                               ccATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATg                                    >  5:347451/1‑149 (MQ=255)
                                                                                                                 ataataTTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGt                                  >  8:70309/1‑149 (MQ=255)
                                                                                                                  taataTTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTc                                 >  8:208080/1‑149 (MQ=255)
                                                                                                                    atatTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGt                               <  5:297422/149‑1 (MQ=255)
                                                                                                                       ttCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATATTTCAg                                                                                                    >  3:120171/1‑77 (MQ=255)
                                                                                                                          aaTGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAAt                         >  6:205430/1‑149 (MQ=255)
                                                                                                                          aaTGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAACATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTa                           >  2:339895/1‑147 (MQ=255)
                                                                                                                           aTGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGACCACTTTTGCCACATACTCATAACGTAAACCAATGGTCGTCGTAATg                        >  1:60199/1‑149 (MQ=255)
                                                                                                                              cagtggaaGCAAACTGGTTGAACTATAGGAGTTTGGTTTGTCGCTGGTCGATGGCGGGGTGATTTTTCAGCATCTCACGTACATCTTAGACAATTACGCCCACTTTTGCCGCATCATCATAACGTTAACCAATGGTCTTGGTAATgcgg                     <  6:40090/142‑1 (MQ=255)
                                                                                                                              aaGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATgcgg                     >  8:111348/1‑149 (MQ=255)
                                                                                                                                    cAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTg               <  1:339895/149‑1 (MQ=255)
                                                                                                                                      ccttaCTGGTTTATTTCAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGt             >  8:340989/5‑149 (MQ=255)
                                                                                                                                       cAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGt             >  8:98918/1‑148 (MQ=255)
                                                                                                                                       cAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGt             >  8:93592/1‑148 (MQ=255)
                                                                                                                                        ttaCTGGTTTATTTCAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGAGTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTg               <  5:257130/143‑1 (MQ=255)
                                                                                                                                        ttaCTGGTTTATATCAACCAGTAAGGTTTGTCGCTGCTCGATGGCCGGGTGATTTTTCAGCATCTCACGAACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATTGACGTGGTAATGCGGCGGtagca             >  6:257130/3‑143 (MQ=255)
                                                                                                                                         aaCTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCAt          <  5:205430/149‑1 (MQ=255)
                                                                                                                                          aCTGGTTTATTTCAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCa           <  7:340989/147‑1 (MQ=255)
                                                                                                                                          aCTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCAtt         >  5:352228/1‑149 (MQ=255)
                                                                                                                                                 gAAATAAACCTGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGCCAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTg  >  2:103615/1‑149 (MQ=255)
                                                                                                                                                 gAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCt   <  4:148070/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTG  >  NC_000913/1394944‑1395237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: