Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,691,089 G→T 5.2% Q507K (CAG→AAG)  bcsB ← regulator of cellulose synthase, cyclic di‑GMP binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,691,0890GT 5.2% 292.3 / 3.0 90Q507K (CAG→AAG) bcsBregulator of cellulose synthase, cyclic di‑GMP binding
Reads supporting (aligned to +/- strand):  ref base G (46/39);  new base T (3/2);  total (49/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCACGGTGATGGTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTGTAATACC  >  NC_000913/3690945‑3691237
                                                                                                                                                |                                                                                                                                                    
tCAAGGTGATGGTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGAGCGTAGATCCGGCATCGGGATGATGTGGTAAAACATCGAGAAGACGATGGTGGAGTCGTCACCAATAACAACATGATTCTGCAcc                                                                                                                                                  >  2:339946/1‑149 (MQ=255)
 cACGTTGATGGTTAGCGACAGATCAGCCATCCTGCTGAATTGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTCGTAATACTTAGATAAGTCGATTGTGGAGTATTCACCAATCACAACATGATTATGCACCg                                                                                                                                                 >  3:85652/1‑149 (MQ=255)
  aCGGTGATGGTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCgg                                                                                                                                                >  1:226482/1‑149 (MQ=255)
  aCGGTGATGGTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCgg                                                                                                                                                >  1:57331/1‑149 (MQ=255)
  aCGGTGATGGTTAGCGACAGATCCGCCATCCGGCTCAAAGGGAAGCCCGCGTTAGCAAAGGCGCGAAGATCCGGCATAGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCAACACATGAATCTGCACCgg                                                                                                                                                >  6:304022/1‑149 (MQ=255)
        aTGGTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGaa                                                                                                                                          <  5:300605/149‑1 (MQ=255)
         tGGTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAg                                                                                                                                         >  3:325540/1‑149 (MQ=255)
          ggTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAgg                                                                                                                                        >  1:154493/1‑149 (MQ=255)
           gTTTGCGAGAGATACGCCCTCCGGCTGAATGGGACGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGGCGTCACGAATCACCACATGATTCTGCACCGGCTGGAAgg                                                                                                                                        <  2:106875/148‑1 (MQ=255)
           gTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGt                                                                                                                                       <  2:154493/149‑1 (MQ=255)
               gCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATa                                                                                                                                   >  1:276158/1‑149 (MQ=255)
                cGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATa                                                                                                                                   >  7:152037/1‑148 (MQ=255)
                  aCAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAg                                                                                                                                >  5:147685/1‑149 (MQ=255)
                  aCAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAg                                                                                                                                <  5:49724/149‑1 (MQ=255)
                    aGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGtt                                                                                                                              <  8:201864/149‑1 (MQ=255)
                      aTCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTAt                                                                                                                            >  1:208562/1‑149 (MQ=255)
                         cGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCa                                                                                                                         >  3:166683/1‑149 (MQ=255)
                         cGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGACTCGTCACCACTCACCACATGATTCTGCACCGGCTGGAAGGTAATACAATTCTCCa                                                                                                                         >  6:78011/1‑149 (MQ=255)
                          gCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCAc                                                                                                                        <  7:158554/149‑1 (MQ=255)
                           ccATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCAcc                                                                                                                       <  7:241025/149‑1 (MQ=255)
                                 ggCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCg                                                                                                                 >  5:219639/1‑149 (MQ=255)
                                 ggCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACAGGCGGGAAGGTAATACAGTTATACACCGAACCg                                                                                                                 >  5:343910/1‑149 (MQ=255)
                                   cTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCGGGAAGGTAATACAGTTATCCACCGAACCGcc                                                                                                               <  6:133426/149‑1 (MQ=255)
                                     gAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCg                                                                                                             >  5:263642/1‑149 (MQ=255)
                                          ggAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGCGCAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCAt                                                                                                         <  2:123512/148‑1 (MQ=255)
                                          ggAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATc                                                                                                        <  8:152037/149‑1 (MQ=255)
                                           gAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCg                                                                                                       >  6:213522/1‑149 (MQ=255)
                                                   cgTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCata                                                                                               <  5:191974/149‑1 (MQ=255)
                                                       aGCAAAGGCGCTTAGATCCGGCTTCGGGATGAAGTTGTAAGACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACTTGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATAc                                                                                            <  5:304022/148‑1 (MQ=255)
                                                       aGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCAGGCATCGGGTTCATATACt                                                                                           >  2:335395/1‑149 (MQ=255)
                                                       aGCAAAGCCGCGTAGAGCCGGCATCGGGATGAATTGCGAAAACTTCGACAAGTCGATGGTGTAGTCGTCACCACGCACCACTTGCTTCTGCACCGGCTGGAAGGTAATTCAGTTATCCACCCCACCGGCCGGCATCGGGTTCATTTAc                                                                                            <  4:284993/148‑1 (MQ=255)
                                                        gCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTc                                                                                          <  1:44317/149‑1 (MQ=255)
                                                         cAAAGGCTCGTAGATCCGGCATCAGGATGAAGAGGTAATACTTCTAGAAGTCGATGTTGGAGTCGTCACCAAAAACCACATTAATCATCACCGGCTGTAAGGTAAAAAAGTTATCCACCGAACCGCCCGGCATCGCGTTCAAAAACTc                                                                                          >  6:54047/1‑148 (MQ=255)
                                                            aGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAg                                                                                      >  3:150325/1‑149 (MQ=255)
                                                                 cgTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGaa                                                                                 <  2:276158/149‑1 (MQ=255)
                                                                            cATCGGTATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCTATCAGCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCtggt                                                                      <  4:85652/149‑1 (MQ=255)
                                                                             aTCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCtggtt                                                                     >  3:320976/1‑149 (MQ=255)
                                                                              tCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCtggttg                                                                    <  4:325540/149‑1 (MQ=255)
                                                                              tCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCCGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCtggttg                                                                    >  5:355254/1‑149 (MQ=255)
                                                                                  gATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTcg                                                                >  5:362077/1‑149 (MQ=255)
                                                                                     gAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCgcgc                                                             >  8:295249/1‑149 (MQ=255)
                                                                                      aaGTGGTAATTCTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCgcgc                                                             <  1:339946/148‑1 (MQ=255)
                                                                                              aTACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCa                                                    <  6:362077/149‑1 (MQ=255)
                                                                                               tACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAg                                                   >  1:23402/1‑149 (MQ=255)
                                                                                                 cTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAgc                                                  <  6:263642/148‑1 (MQ=255)
                                                                                                  ttCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCCCATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAgcgc                                                >  8:197825/1‑149 (MQ=255)
                                                                                                       gaAGTCGATGGTGGATTCGTCACCAATCACCACATGATTCTTCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTc                                                                                    <  3:215333/108‑1 (MQ=255)
                                                                                                       gaAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTTCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTc                                                                                    <  3:210781/108‑1 (MQ=255)
                                                                                                       gaAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTTCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTc                                                                                    >  4:210781/1‑108 (MQ=255)
                                                                                                       gaAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTTCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTc                                                                                    >  4:215333/1‑108 (MQ=255)
                                                                                                       gaAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGaa                                           >  3:277199/1‑149 (MQ=255)
                                                                                                       gaAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGa                                            >  8:65396/1‑148 (MQ=255)
                                                                                                         aGTCGATGGTGGAGTCGTCACCAATCCCCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCCGaa                                           >  4:135299/1‑147 (MQ=255)
                                                                                                          gTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGaa                                           >  7:134320/1‑146 (MQ=255)
                                                                                                          gTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAACTCGAAGCGCAGCTGGTTGGTCGCGCCCATTTTCAGCGCCGGaa                                           >  8:333303/1‑146 (MQ=255)
                                                                                                           tCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATaga                                       <  2:226482/149‑1 (MQ=255)
                                                                                                           tCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGATATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATaga                                       <  6:111021/149‑1 (MQ=255)
                                                                                                             taTGGTGGAGTCGTCACCAATCACCACAGGATTCTGCACCGTCTGGAAGGTAATACAGTTATCCTCCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAgaga                                     <  6:343910/148‑1 (MQ=255)
                                                                                                             gATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGCACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCACCGGGTTGGTCCCGCCCAGTTTCAGCGCCGGAATagag                                      >  4:46227/1‑148 (MQ=255)
                                                                                                             gATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGACCCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATagag                                      <  3:46227/148‑1 (MQ=255)
                                                                                                             gATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGGCGCGCCCAGTTTCAGCGCCGGAATAgaga                                     <  6:219639/149‑1 (MQ=255)
                                                                                                             gATGGTGGAGTCCTCACCAATCACCACATGATTCTGCACCGGCGGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCATCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGATTAgaga                                     <  4:196865/149‑1 (MQ=255)
                                                                                                              aTGGTGGAGTCTTCACCAATCACCACATGATTCTGCACCGGCTGTAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCTAAGGGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGAc                                    <  5:78011/149‑1 (MQ=255)
                                                                                                                  tggAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCt                                >  4:14051/1‑149 (MQ=255)
                                                                                                                      gtcgtcACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGttt                            >  4:173703/1‑149 (MQ=255)
                                                                                                                       tcgtcACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGaa                                           >  6:152273/1‑133 (MQ=255)
                                                                                                                       tcgtcACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGaa                                           <  5:152273/133‑1 (MQ=255)
                                                                                                                       tcgtcACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGtttt                           >  6:309326/1‑149 (MQ=255)
                                                                                                                       tcgtcACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGtttt                           <  4:277199/149‑1 (MQ=255)
                                                                                                                       tcggcACCAATCACCTCATGAGTCTGCACCGGCTGGAAGGTACTACAGTTATCCACCGAACCGCCCGGCATCGCGTGCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTGGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGtttt                           <  4:182141/149‑1 (MQ=255)
                                                                                                                         gtcACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGc                         >  7:137916/1‑149 (MQ=255)
                                                                                                                            aCCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCAt                      >  5:9698/1‑149 (MQ=255)
                                                                                                                              cAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATc                     >  5:306794/1‑148 (MQ=255)
                                                                                                                              cAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATc                     >  2:11563/1‑148 (MQ=255)
                                                                                                                               aaccaccacATGCTGCTGCACGGGCTGGAAGGTAATCCCGGTCGCCACCGAACCGCCCGGCATCGGGTTCATATACGCTAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTgccagcca                   <  2:301945/146‑1 (MQ=255)
                                                                                                                                aTCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAg                  <  2:57331/149‑1 (MQ=255)
                                                                                                                                    ccacATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCaaa              >  8:256520/1‑149 (MQ=255)
                                                                                                                                     cacaTGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAAc             >  7:341162/1‑149 (MQ=255)
                                                                                                                                       caTGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCt           >  5:358834/1‑149 (MQ=255)
                                                                                                                                        aTGATTCTGCACCGGCTGGAATGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCtt          <  5:54047/149‑1 (MQ=255)
                                                                                                                                        aTGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGtttt                           <  7:145778/132‑1 (MQ=255)
                                                                                                                                        aTGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGtttt                           >  8:145778/1‑132 (MQ=255)
                                                                                                                                        aTGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCtt          <  6:9698/149‑1 (MQ=255)
                                                                                                                                         tgcttCTGCACCGGCTGGAAGGTAATACTGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTg         <  5:332728/146‑1 (MQ=255)
                                                                                                                                          gATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTg         >  6:348956/1‑148 (MQ=255)
                                                                                                                                           aTTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTGTa       <  7:65396/149‑1 (MQ=255)
                                                                                                                                           aTTCTGCACCGGCTGGAAGGTAATACAGTTAACCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCACTTTCAGCGCCGCAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTGt        >  2:35084/1‑148 (MQ=255)
                                                                                                                                            ttCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTGTaa      <  5:309326/149‑1 (MQ=255)
                                                                                                                                            ttCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTGTaa      <  4:150325/149‑1 (MQ=255)
                                                                                                                                             tCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTGTaa      >  2:68193/1‑148 (MQ=255)
                                                                                                                                                gCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTGTAATAcc  >  7:54695/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCACGGTGATGGTTTGCGACAGATCCGCCATCCGGCTGAATGGGAAGCCCGCGTTAGCAAAGGCGCGTAGATCCGGCATCGGGATGAAGTGGTAATACTTCGAGAAGTCGATGGTGGAGTCGTCACCAATCACCACATGATTCTGCACCGGCTGGAAGGTAATACAGTTATCCACCGAACCGCCCGGCATCGGGTTCATATACTCAAAGTCGAAGCGCAGCTGGTTGGTCGCGCCCAGTTTCAGCGCCGGAATAGAGACATCTGTTTTGCCATCCAGCAAACCTTGTAATACC  >  NC_000913/3690945‑3691237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: