Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,107,7180TA22.2% 52.5 / 10.4 27F455Y (TTC→TAC) ‡clsCstationary phase cardiolipin synthase 3
Reads supporting (aligned to +/- strand):  ref base T (10/11);  new base A (3/3);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACTTCAGTAACGGGATGCGCAAAT  >  NC_000913/1107580‑1107866
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aGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCgg                                                                                                                                            <  5:57433/149‑1 (MQ=255)
aGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCgg                                                                                                                                            <  2:88780/149‑1 (MQ=255)
  ttAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGc                                                                                                                                              >  1:118949/1‑145 (MQ=255)
  ttAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGc                                                                                                                                              <  2:118949/145‑1 (MQ=255)
      ttGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATg                                                                                                                                      <  4:35368/149‑1 (MQ=255)
           aaaCGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCTTCCAGAAACTGGTGGCGGGttctttttt                                                                                                                                  <  8:87303/148‑8 (MQ=38)
            aaCGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGa                                                                                                                                <  6:39643/149‑1 (MQ=255)
                            ccAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCTTCCAGAAACTGGTGGCGGGttctttt                                                                                                                                    <  8:9954/129‑6 (MQ=38)
                            ccAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCTTCCAGAAACTGGTGGCGGGttctttt                                                                                                                                    >  7:9954/1‑124 (MQ=38)
                                  tgatgCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTg                                                                                                           <  1:45314/148‑1 (MQ=255)
                                                                              aCTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGt                                                              >  2:99223/1‑149 (MQ=255)
                                                                                           tCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTaa                                                  >  6:164532/1‑148 (MQ=255)
                                                                                                  aagaaagaGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtctc                                                          >  2:11750/1‑133 (MQ=255)
                                                                                                   agaaagaGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGtt                                         >  1:25052/1‑149 (MQ=255)
                                                                                                     aaagaGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTa                                       <  8:19760/149‑1 (MQ=255)
                                                                                                      aagaGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTAc                                      <  5:164532/149‑1 (MQ=255)
                                                                                                       agagATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTAc                                      >  7:93111/1‑148 (MQ=255)
                                                                                                               ttCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAAc                              <  1:99223/148‑1 (MQ=255)
                                                                                                                   cAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATc                                                                >  8:61405/1‑110 (MQ=255)
                                                                                                                   cAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATc                                                                <  7:61405/110‑1 (MQ=255)
                                                                                                                     aaaaaGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACt                       >  2:11307/1‑149 (MQ=255)
                                                                                                                       aaaGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACtt                      >  6:158732/1‑148 (MQ=255)
                                                                                                                        cataaCCCGCTTCCAGAAACTGGTGGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACtt                      >  5:36254/4‑147 (MQ=255)
                                                                                                                        cataaCCCGCTTCCAGAAACTGGTGGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACtt                      >  7:46391/4‑147 (MQ=255)
                                                                                                                        cataaCCCGCTTCCAGAAACTGGTGGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACTTCa                    <  6:36254/146‑1 (MQ=255)
                                                                                                                           aaCCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGGTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACACCCGGTTTACCAGAGAACAAAAACTTCAGt                  >  1:124225/1‑148 (MQ=255)
                                                                                                                                gCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACTTCAGTAACggg            <  5:158732/149‑1 (MQ=255)
                                                                                                                                          tCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGAGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACTTCAGTAACGGGATGCGCAAAt  >  1:14450/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
AGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACCGGTTTACCAGAGAACAAAAACTTCAGTAACGGGATGCGCAAAT  >  NC_000913/1107580‑1107866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑