Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,806,335 G→T intergenic (‑252/‑35) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,3350GT100.0% 77.4 / NA 23intergenic (‑252/‑35)ydiY/pfkBacid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/11);  total (12/11)

AATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTC  >  NC_000913/1806192‑1806474
                                                                                                                                               |                                                                                                                                           
aaTAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTATGTGc                                                                                                                                         >  7:99476/1‑148 (MQ=255)
             tGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAg                                                                                                                           >  4:22262/1‑149 (MQ=255)
               tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGcc                                                                                                                         >  2:167058/1‑149 (MQ=255)
                  tCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCta                                                                                                                       >  3:96359/1‑148 (MQ=255)
                      aTTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATag                                                                                                                    <  1:167058/147‑1 (MQ=255)
                        ttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATagga                                                                                                                  <  8:69358/147‑1 (MQ=255)
                        ttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATagga                                                                                                                  >  7:69358/1‑147 (MQ=255)
                           aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGaaa                                                                                                             <  8:95043/149‑1 (MQ=255)
                              ttttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAatg                                                                                                           <  3:22262/148‑1 (MQ=255)
                               tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAatga                                                                                                          <  4:96359/148‑1 (MQ=255)
                                  gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                           <  2:75471/112‑1 (MQ=255)
                                  gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                           >  1:75471/1‑112 (MQ=255)
                                        gCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATCAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                                       <  7:149090/142‑1 (MQ=255)
                                        gCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                                       >  8:149090/1‑142 (MQ=255)
                                                    aaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                                     <  2:70768/148‑1 (MQ=255)
                                                    aaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                                     >  1:70768/1‑148 (MQ=255)
                                                                tttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCct                                                                         <  8:99476/148‑1 (MQ=255)
                                                                                    aaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                                    >  5:146047/1‑149 (MQ=255)
                                                                                      aTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                                                  <  6:146047/149‑1 (MQ=255)
                                                                                            aataaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCa                                             >  1:117045/1‑148 (MQ=255)
                                                                                                    tCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATcc                                    >  7:13072/1‑149 (MQ=255)
                                                                                                      cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATccc                                   >  4:100707/1‑148 (MQ=255)
                                                                                                                                      cGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTc  <  3:100707/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                           
AATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTC  >  NC_000913/1806192‑1806474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: