Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,183,106 C→T 100% S621F (TCC→TTC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,183,1060CT100.0% 429.2 / ‑6.7 123S621F (TCC→TTC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (61/61);  minor base G (1/0);  total (62/61)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 123 total aligned reads displayed.

AACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCG  >  NC_000913/4182961‑4183251
                                                                                                                                                 |                                                                                                                                                 
aaCTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCa                                                                                                                                                 <  5:179386/148‑1 (MQ=255)
 aCTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAAc                                                                                                                                               <  3:216367/149‑1 (MQ=255)
 aCTCTCTGTCCGTGTACGCACAGACTAACGAAAACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAAc                                                                                                                                               <  2:131203/149‑1 (MQ=255)
       tGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAACTGTCTGCTAGCGAAGAAGGAAACTACGTTATCGCCCAGGCGAACTTCAACTTGGAt                                                                                                                                         >  2:343901/1‑149 (MQ=255)
         tCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGa                                                                                                                                          <  5:185313/146‑1 (MQ=255)
          ccGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATg                                                                                                                                        >  8:294693/1‑147 (MQ=255)
            gtgtACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATgaaga                                                                                                                                    <  5:80153/149‑1 (MQ=255)
            gtgtACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATgaaga                                                                                                                                    <  5:52029/149‑1 (MQ=255)
                  gCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCa                                                                                                                              >  8:74769/1‑149 (MQ=255)
                  gCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCa                                                                                                                              >  2:141090/1‑149 (MQ=255)
                    acaGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTACCTCCCGAATTTCCCTACGTGGGGGCTTTCGAAGAAGGCAACTACGTTATCGCCCGGGCGAACTGCAACTTGGATGAAGACGGcacgt                                                                                                                            >  6:354018/1‑145 (MQ=255)
                    acaGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACt                                                                                                                            >  8:18014/1‑149 (MQ=255)
                          tAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCg                                                                                                                         >  8:121659/1‑146 (MQ=255)
                          tAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCg                                                                                                                         >  3:246403/1‑146 (MQ=255)
                          tAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCg                                                                                                                         >  5:268265/1‑146 (MQ=255)
                          tAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCg                                                                                                                         >  4:321036/1‑146 (MQ=255)
                            aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaa                                                                                                                    <  6:174987/149‑1 (MQ=255)
                            aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaa                                                                                                                    <  6:41107/149‑1 (MQ=255)
                            aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTag                                                                                                                      <  7:74769/147‑1 (MQ=255)
                            aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTag                                                                                                                      >  1:283959/1‑147 (MQ=255)
                                aTACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAGCTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGAcc                                                                                                                >  7:24015/1‑149 (MQ=255)
                                aTACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGAcc                                                                                                                <  4:246403/149‑1 (MQ=255)
                                aTACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTACTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGAcc                                                                                                                >  5:299562/1‑149 (MQ=255)
                                   cGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTgg                                                                                                             <  8:52722/149‑1 (MQ=255)
                                   cGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTg                                                                                                              <  6:268265/148‑1 (MQ=255)
                                    ggCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGt                                                                                                            <  7:294693/149‑1 (MQ=255)
                                      cTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTaa                                                                                                          <  7:121659/149‑1 (MQ=255)
                                       ttCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGaagaag                                                                                                                                  >  2:159771/1‑124 (MQ=255)
                                       ttCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGaagaag                                                                                                                                  <  1:159771/124‑1 (MQ=255)
                                        tCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACt                                                                                                        >  2:25100/1‑149 (MQ=255)
                                          cTTGAGACTGCGTATCGTAAAGTGACCGAGGGTGTTGTAACTGACGAAATTCACTACATGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGTCGAACTTAAACTTGGAt                                                                                                                                         >  6:141892/1‑114 (MQ=255)
                                          cTTGAGACTCCGTATCGTAAAGTGACCGACGGTTTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGACGAAGGCAACTAGGTTATCGCCCAGGCGAACTTCAACTTGGAt                                                                                                                                         <  5:141892/114‑1 (MQ=255)
                                          cTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTg                                                                                                      >  4:100077/1‑149 (MQ=255)
                                          cTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTg                                                                                                      >  7:202600/1‑149 (MQ=255)
                                           ttGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGc                                                                                                     >  8:363749/1‑149 (MQ=255)
                                              agaCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCg                                                                                                   <  7:18014/148‑1 (MQ=255)
                                                    cGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTTCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCaaa                                                                                            <  6:299562/149‑1 (MQ=255)
                                                    cGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaa                                                                                                                    >  3:312305/1‑125 (MQ=255)
                                                    cGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaa                                                                                                                    <  4:312305/125‑1 (MQ=255)
                                                      tATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAgg                                                                                          >  8:166635/1‑149 (MQ=255)
                                                      tATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAgg                                                                                          >  8:55061/1‑149 (MQ=255)
                                                      tATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGACCAGTCGAAATTCAACTTGCAAGAAGAAGCCCACTTCGTAGAATACCTGGTAACTTGCCGTAGCAAAgg                                                                                          >  1:116548/1‑149 (MQ=255)
                                                        tCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCg                                                                                                                         >  8:54042/1‑116 (MQ=255)
                                                        tCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCg                                                                                                                         <  7:54042/116‑1 (MQ=255)
                                                        tCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACt                                                                                                        <  3:80154/133‑1 (MQ=255)
                                                          gTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGaagaag                                                                                                                                  <  1:164012/105‑1 (MQ=255)
                                                          gTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGaagaag                                                                                                                                  >  2:164012/1‑105 (MQ=255)
                                                            aaaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAgg                                                                                          <  3:351450/143‑1 (MQ=255)
                                                            aaaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAgg                                                                                          >  4:351450/1‑143 (MQ=255)
                                                            aaaCTGACCGACGGTGATGAAACTGACGAAATACACTACCTGTCTGCAATCCAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGACGGCCACTTCGTAGAAGCCCTGGTAACTTGCCGTAGCAAAGGCGAATc                                                                                    >  8:115984/1‑149 (MQ=255)
                                                              aGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCa                                                                                  <  3:100077/149‑1 (MQ=255)
                                                                  aCCGACGGTGTTGTAGCTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCtt                                                                              <  8:24015/149‑1 (MQ=255)
                                                                    cGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGt                                                                            >  5:116542/1‑149 (MQ=255)
                                                                    cGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGt                                                                            >  5:399798/1‑149 (MQ=255)
                                                                        ggTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAg                                                                        >  1:225181/1‑149 (MQ=255)
                                                                          tgttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGcc                                                                      <  1:25100/149‑1 (MQ=255)
                                                                            ttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCa                                                                         >  5:91712/1‑144 (MQ=255)
                                                                            ttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCa                                                                         <  6:91712/144‑1 (MQ=255)
                                                                            ttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCcgc                                                                    >  7:166933/1‑149 (MQ=255)
                                                                              gtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaag                                                                                                                   <  5:360817/100‑1 (MQ=255)
                                                                              gtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTagaag                                                                                                                   >  6:360817/1‑100 (MQ=255)
                                                                                 aCTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGAc                                                                 >  6:171395/1‑147 (MQ=255)
                                                                                 aCTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCa                                                               <  5:120977/149‑1 (MQ=255)
                                                                                     aCGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGc                                                                                         >  5:31706/1‑119 (MQ=255)
                                                                                     aCGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGc                                                                                         <  6:31706/119‑1 (MQ=255)
                                                                                     aCGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAgg                                                             <  8:202600/147‑1 (MQ=255)
                                                                                      cGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATgaaga                                                                                                                                    <  5:190048/75‑1 (MQ=255)
                                                                                       gAAATTCACTACCTTTCTGCTATCGAAGAAGGCAACTACGTTATCGCGCAGGCGAACTTCAACTTGGATGAAGAAGGCCTCttgg                                                                                                                         <  2:238702/85‑3 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGa                                                         <  1:100969/149‑1 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCAAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGACGCGACCAGGttta                                                         >  6:286702/1‑147 (MQ=255)
                                                                                       gAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATAGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCg                                                                                                                         >  1:238702/1‑85 (MQ=255)
                                                                                           ttCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACt                                                       <  3:309422/147‑1 (MQ=255)
                                                                                           ttCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACt                                                       >  4:309422/1‑147 (MQ=255)
                                                                                              aCTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAt                                                   <  8:166933/148‑1 (MQ=255)
                                                                                                  ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCa                                                                                                                              >  8:383918/1‑69 (MQ=255)
                                                                                                  ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCa                                                                                                                              >  8:389502/1‑69 (MQ=255)
                                                                                                  ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCa                                                                                                                              <  7:383918/69‑1 (MQ=255)
                                                                                                  ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCa                                                                                                                              <  7:389502/69‑1 (MQ=255)
                                                                                                  ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGa                                                <  7:76960/147‑1 (MQ=255)
                                                                                                  ccTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGAc                                               <  2:225181/148‑1 (MQ=255)
                                                                                                      tCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTAt                                           <  2:283959/148‑1 (MQ=255)
                                                                                                      tCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTAt                                           <  6:59572/148‑1 (MQ=255)
                                                                                                        tGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCa                                        >  7:409246/1‑149 (MQ=255)
                                                                                                         gCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAc                                       >  4:174836/1‑149 (MQ=255)
                                                                                                          cTATCGAAGAAGGCAACTACGTTATCGCCAAGGCGAACTTCAAATTGGATGAAGAAGGCCAGTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAcc                                      <  2:116548/149‑1 (MQ=255)
                                                                                                           tATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCAcc                                      >  4:168667/1‑148 (MQ=255)
                                                                                                                     ggCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAggtgg                            >  4:174581/1‑148 (MQ=255)
                                                                                                                      gCAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAggtgg                            >  6:261638/1‑147 (MQ=255)
                                                                                                                       cAACTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTAt                         <  5:171395/149‑1 (MQ=255)
                                                                                                                        aaCTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATc                        >  4:31148/1‑149 (MQ=255)
                                                                                                                         aCTACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATcc                       >  4:91700/1‑149 (MQ=255)
                                                                                                                           tACGTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCGTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGt                     >  6:199509/1‑149 (MQ=255)
                                                                                                                              gTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGAc                                               <  3:272302/120‑1 (MQ=255)
                                                                                                                              gTTATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCgg                  <  3:84346/149‑1 (MQ=255)
                                                                                                                               ttATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGt                 <  1:169232/149‑1 (MQ=255)
                                                                                                                                tATCGCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTg                >  6:178276/1‑149 (MQ=255)
                                                                                                                                    gCCCAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCCGCAGGTGGTATCCGTCGGTGCGTc            >  3:150442/1‑149 (MQ=255)
                                                                                                                                     cccAGGCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTcc           <  3:174581/149‑1 (MQ=255)
                                                                                                                                          gCGAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGAt      >  5:9947/1‑149 (MQ=255)
                                                                                                                                            gAACTTCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATcc    >  6:204177/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCG  >  NC_000913/4182961‑4183251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑