Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,521,385 G→T 5.4% A212A (GCC→GCA)  yjhF ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,521,3850GT 5.4% 312.4 / 3.9 91A212A (GCC→GCA) yjhFputative transporter
Reads supporting (aligned to +/- strand):  ref base G (41/45);  new base T (2/3);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

ATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGC  >  NC_000913/4521237‑4521531
                                                                                                                                                    |                                                                                                                                                  
aTAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGagagag                                                                                                                                                    >  1:262072/1‑149 (MQ=255)
     ccTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCtat                                                                                                                                               <  4:380982/149‑1 (MQ=255)
       tGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAgg                                                                                                                                                   <  6:47914/143‑1 (MQ=255)
       tGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAgg                                                                                                                                                   >  5:47914/1‑143 (MQ=255)
       tGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTatat                                                                                                                                             >  3:90330/1‑149 (MQ=255)
         aaTCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTc                                                                                                                                           <  1:109637/149‑1 (MQ=255)
                 gCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCttt                                                                                                                                   >  8:23769/1‑149 (MQ=255)
                    cTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCttttt                                                                                                                                 >  7:318761/1‑148 (MQ=255)
                    cTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCttttt                                                                                                                                 >  2:77410/1‑148 (MQ=255)
                     ttttGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCtttttt                                                                                                                                >  7:346428/1‑148 (MQ=255)
                       ttGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTaa                                                                                                                              <  3:222738/148‑1 (MQ=255)
                        tGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTaaa                                                                                                                             <  1:307537/148‑1 (MQ=255)
                        tGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTaaa                                                                                                                             <  7:407483/148‑1 (MQ=255)
                        tGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTaaa                                                                                                                             <  1:77410/148‑1 (MQ=255)
                        tGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTaaa                                                                                                                             <  2:262072/148‑1 (MQ=255)
                        tGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTaaa                                                                                                                             <  4:138361/148‑1 (MQ=255)
                         ggCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAg                                                                                                                            <  8:374775/148‑1 (MQ=255)
                           cATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCg                                                                                                                         >  8:321008/1‑149 (MQ=255)
                            aTGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGt                                                                                                                        >  7:72463/1‑149 (MQ=255)
                            aTGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGt                                                                                                                        >  2:353054/1‑149 (MQ=255)
                             tGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTaaa                                                                                                                             <  2:34180/143‑1 (MQ=255)
                             tGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTaaa                                                                                                                             >  1:34180/1‑143 (MQ=255)
                             tGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGc                                                                                                                          <  7:64575/146‑1 (MQ=255)
                             tGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGc                                                                                                                          >  8:64575/1‑146 (MQ=255)
                             tGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTc                                                                                                                       >  3:177680/1‑149 (MQ=255)
                              gAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTcc                                                                                                                      >  4:271289/1‑149 (MQ=255)
                                       ggATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATg                                                                                                             >  3:52704/1‑149 (MQ=255)
                                            atgGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTg                                                                                                        <  4:228839/149‑1 (MQ=255)
                                            atgGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTg                                                                                                        >  5:351972/1‑149 (MQ=255)
                                             tgGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTgg                                                                                                       >  8:390987/1‑149 (MQ=255)
                                               gTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGc                                                                                                      <  7:321008/148‑1 (MQ=255)
                                                 gACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAg                                                                                                    >  5:395060/1‑148 (MQ=255)
                                                      ccGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTa                                                                                              >  4:123860/1‑149 (MQ=255)
                                                       cGCCATCAGGATCACCGTCAGCATTGCAGTGAGAAAACTTATCCCAAAGCCTGGCATATCGCGTTCTCTGAATCGGCGGGTCGCGCCGAGAGAGTCTATATTCCCCTCTTTTTTAAAGGCGTACGGAATAATTCGCTGGCATAATTTAt                                                                                             <  4:21301/149‑1 (MQ=255)
                                                       cGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTAt                                                                                             <  3:271289/149‑1 (MQ=255)
                                                        gCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTAtt                                                                                            <  6:351972/149‑1 (MQ=255)
                                                          cATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCa                                                                                          <  5:115621/149‑1 (MQ=255)
                                                           aTCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCaa                                                                                         <  7:143112/149‑1 (MQ=255)
                                                            tCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCaa                                                                                         >  3:190951/1‑148 (MQ=255)
                                                             cAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGAGTCCGGAATAATGCGCTGGCAGAATTTCTTCAATa                                                                                       >  4:320529/1‑149 (MQ=255)
                                                              aGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATa                                                                                       <  1:269519/148‑1 (MQ=255)
                                                               ggATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCtt                                                                                                                                    >  3:303115/1‑102 (MQ=255)
                                                               ggATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCtt                                                                                                                                    <  4:303115/102‑1 (MQ=255)
                                                                gATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCg                                                                                    >  8:380392/1‑149 (MQ=255)
                                                                gATCAACGTCAGCATAGCCGTGAGAACACTGATCCCAAAGCGAGGCAGTTCGCTAACGCAGAATCGGCGGGTCGCGCCGAGACAGGATATATTCAAGACTTATTTAAACGCCTCCAGAAAAAAGAGCAGCCACAATATATACAAAACCg                                                                                    >  2:345163/1‑149 (MQ=255)
                                                                  tCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTAt                                                                                             >  4:273190/1‑138 (MQ=255)
                                                                  tCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTAt                                                                                             <  3:273190/138‑1 (MQ=255)
                                                                  tCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCGCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCggg                                                                                  <  5:61634/149‑1 (MQ=255)
                                                                        gcagcaTTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCa                                                                             >  7:107362/1‑148 (MQ=255)
                                                                         cagcaTTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCaaa                                                                           <  4:52704/149‑1 (MQ=255)
                                                                                gCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATa                                                                      >  2:71499/1‑147 (MQ=255)
                                                                                accGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATaa                                                                     <  3:352652/147‑1 (MQ=255)
                                                                                      agaATAGTGTTCCCTATGGCTGGCATTTCGCTTTCGCTGTATCGGGGGGTGGCGCCGAGAGAGTCTATATTCCCCTCTTTTTTAAATGCGTGCGGAATATTGCGCTGGCAGAATTTTTTCAATACCGGGGGGCAATGATTAAAGGttt                                                               <  8:107362/148‑2 (MQ=255)
                                                                                      agaAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTg                                                               <  6:395060/148‑1 (MQ=255)
                                                                                       gaAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGa                                                             <  7:23769/149‑1 (MQ=255)
                                                                                        aaaaCTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGaa                                                            <  8:346428/149‑1 (MQ=255)
                                                                                           aCTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGc                                                                                                                          <  8:29872/84‑1 (MQ=255)
                                                                                           aCTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGc                                                                                                                          >  7:29872/1‑84 (MQ=255)
                                                                                               aTCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCg                                                       <  8:318761/147‑1 (MQ=255)
                                                                                               aTCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGa                                                      <  4:190951/148‑1 (MQ=255)
                                                                                                  ccAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAAt                                                  <  1:353054/149‑1 (MQ=255)
                                                                                                   cAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAAt                                                  >  1:227018/1‑148 (MQ=255)
                                                                                                     aaGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGGCGCGCCGAGAGATGCTATATTCCCCTCTTTTTTAAAGGCGTCGGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGAt                                               <  4:263984/149‑1 (MQ=255)
                                                                                                        ccTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATAcc                                            <  8:1583/149‑1 (MQ=255)
                                                                                                        ccTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATAcc                                            <  3:123860/149‑1 (MQ=255)
                                                                                                          tGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGATGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCg                                           >  3:358441/1‑148 (MQ=255)
                                                                                                          tGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGATGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCg                                           >  3:352904/1‑148 (MQ=255)
                                                                                                          tGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCg                                           >  8:93124/1‑148 (MQ=255)
                                                                                                          tGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCg                                           >  5:239910/1‑148 (MQ=255)
                                                                                                           ggCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGTCTATATTCCCCTCTTTTTTAAATGCGTCCGGAATAATGCGATGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATTCCGACAATGATACCGta                                         >  2:196423/1‑149 (MQ=255)
                                                                                                            gCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGATGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGta                                         <  4:358441/148‑1 (MQ=255)
                                                                                                            gCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGATGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGta                                         <  4:352904/148‑1 (MQ=255)
                                                                                                            gCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGTAATGCCGACAATGATACCGta                                         <  2:227018/148‑1 (MQ=255)
                                                                                                              aTTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTatat                                      >  6:89675/1‑149 (MQ=255)
                                                                                                              aTTTCACTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTAt                                                                                             >  8:71989/1‑94 (MQ=255)
                                                                                                                 tcgctttcgctGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGGAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAg                                   >  3:231972/1‑149 (MQ=255)
                                                                                                                      ttcgctGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGaa                                                            <  2:265204/119‑1 (MQ=255)
                                                                                                                      ttcgctGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGaa                                                            >  1:265204/1‑119 (MQ=255)
                                                                                                                         gctGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGa                                                      <  2:241442/122‑1 (MQ=255)
                                                                                                                         gctGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGa                                                      >  1:241442/1‑122 (MQ=255)
                                                                                                                         gctGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATc                           >  5:337654/1‑149 (MQ=255)
                                                                                                                          ctGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATcc                          >  1:169372/1‑149 (MQ=255)
                                                                                                                           tGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGc                                                         >  5:227194/1‑117 (MQ=255)
                                                                                                                           tGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGc                                                         <  6:227194/117‑1 (MQ=255)
                                                                                                                             aaTCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTa                        >  5:388279/1‑148 (MQ=255)
                                                                                                                             aaTCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTAc                       <  8:72463/149‑1 (MQ=255)
                                                                                                                              aTCGGCGGGTCGCGCGGAGAGAGGGTTTATTCCCCTCTTTTTTAAAGGCTTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTAc                       <  3:320529/148‑1 (MQ=255)
                                                                                                                               tCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACAt                     >  8:331438/1‑149 (MQ=255)
                                                                                                                               tCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGAAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACAt                     <  5:89675/149‑1 (MQ=255)
                                                                                                                                  gcggGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAAGCCTACATCAg                  >  1:368750/1‑149 (MQ=255)
                                                                                                                                     ggTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAgcgc               <  7:116640/149‑1 (MQ=255)
                                                                                                                                       tCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCa             <  7:380392/149‑1 (MQ=255)
                                                                                                                                           gcCGAGAGAGGCTCTATTCCCCTCTTTTATAAAGCCGGCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAAAAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATCtt         <  4:231972/149‑1 (MQ=255)
                                                                                                                                              gagagaGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTccc      <  6:337654/149‑1 (MQ=255)
                                                                                                                                                   agGCTAAATTCCCGTCTTTTTTAAAGGCGTCAGGTATTATGCGCTGGCAGAATTTATTCAATACCGGGCCGCTAAGAATAAAGGTTGGAATGCCGACAATGATACAGTATATCAGTACCAATCCTACATCAGCGCCATTTTCCCTGGc  <  1:345163/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATAAGCCTGAATCCGCGGCACTTTTGGCATGAGTCATCTGGATGATGGTGACAACCGCCATCAGGATCACCGGCAGCATTGCCGTGAGAAAACTGATCCCAAAGCCTGGCATTTCGCTTTCGCTGAATCGGCGGGTCGCGCCGAGAGAGGCTATATTCCCCTCTTTTTTAAAGGCGTCCGGAATAATGCGCTGGCAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGC  >  NC_000913/4521237‑4521531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑