Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 777,363 C→T 6.2% A341V (GCC→GTC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913777,3630CT 6.2% 204.2 / 2.9 64A341V (GCC→GTC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base C (30/30);  new base T (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

ATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCT  >  NC_000913/777225‑777511
                                                                                                                                          |                                                                                                                                                    
atgatATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGAtt                                                                                                                                            <  3:187295/149‑1 (MQ=255)
atgatATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGAt                                                                                                                                             >  6:136358/1‑148 (MQ=255)
    tatTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAAt                                                                                                                                        >  5:7035/1‑149 (MQ=255)
      ttttCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGTGGCCGATATCAATAACTATGCCGGGCAGATTAAATCt                                                                                                                                      <  6:387702/149‑1 (MQ=255)
      ttttCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCt                                                                                                                                      <  4:259426/149‑1 (MQ=255)
         tCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGc                                                                                                                                    <  7:174413/148‑1 (MQ=255)
             tGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATc                                                                                                                                >  4:294577/1‑148 (MQ=255)
                gCTAAGCTCTGTGAAGAATGCACCGAAAACGGGTGGAGGGGCGAAATGGAACACTGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCACTAACTATGCCGGGCAGATTAAATCTGCTATCGaaa                                                                                                                            <  1:13028/149‑1 (MQ=255)
                 cTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGTGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaag                                                                                                                           >  2:139872/1‑149 (MQ=255)
                  tAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaag                                                                                                                           >  7:21229/1‑148 (MQ=255)
                    aGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaag                                                                                                                           <  8:21229/146‑1 (MQ=255)
                      ctctGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAATACAATGGCGCATCAGGGGCCGATATCAATATCTATGCCGGGCAGATTAATTCTGCTATCGAaagtaagt                                                                                                                      >  8:380724/1‑149 (MQ=255)
                         tgttAAGAATGCACCGATCACGGGGTGAGTGGCGAAAGGGAACAATGCTTCGGCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGc                                                                                                                                    <  6:181353/129‑1 (MQ=255)
                         tGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTc                                                                                                                    >  1:125878/1‑148 (MQ=255)
                         tGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGAGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGc                                                                                                                                    >  5:181353/1‑132 (MQ=255)
                           gTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTAt                                                                                                                 <  6:128014/149‑1 (MQ=255)
                              aGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGa                                                                                                               <  8:129844/148‑1 (MQ=255)
                                 aTGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaagtaa                                                                                                                        <  4:69289/136‑1 (MQ=255)
                                 aTGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAaagtaa                                                                                                                        >  3:69289/1‑136 (MQ=255)
                                     aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGTCGGGCAGATTAAATCTGCTATCGaaa                                                                                                                            >  5:268929/1‑128 (MQ=255)
                                     aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGTCGGGCAGATTAAATCTGCTATCGaaa                                                                                                                            <  4:22872/128‑1 (MQ=255)
                                     aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGTCGGGCAGATTAAATCTGCTATCGaaa                                                                                                                            >  3:22872/1‑128 (MQ=255)
                                     aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGTCGGGCAGATTAAATCTGCTATCGaaa                                                                                                                            <  6:268929/128‑1 (MQ=255)
                                     aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGt                                                                                                       >  6:180500/1‑149 (MQ=255)
                                                ggggAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAGGTTCTATGACGCATCGTCCTATGCAGGc                                                                                            <  6:7035/149‑1 (MQ=255)
                                                   gAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCaaa                                                                                         <  5:180500/149‑1 (MQ=255)
                                                     ggggCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAAc                                                                                       <  5:136358/149‑1 (MQ=255)
                                                     ggggCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAAc                                                                                       <  1:199187/149‑1 (MQ=255)
                                                      gggCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAATcc                                                                                      <  2:125878/149‑1 (MQ=255)
                                                      gggCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATAAATAACTATGCCGGGCAGATTAAAGCTGCTATCGAAAGTAAGATCTATGACGCATCGTCCGATGCAGGCAAAAcc                                                                                      >  6:210170/1‑149 (MQ=255)
                                                            aaGGGAACAATGCTTCGCCTTCCGTGAGTGGTAATACTAAAAACAATGGATCATCAGGTGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACTCATAGTCCTATGCAGGCAAAACCTGTa                                                                                  <  4:52277/147‑1 (MQ=255)
                                                                     aTGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCAt                                                                        >  7:338766/1‑148 (MQ=255)
                                                                        cTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATTTCAATAACTATGCCGGGCAGATTAAATCTGCTATCTAAAGTAAGTTCTATTAATCATCGAACTATGCAGGCAAACCATGTACAATGCGCATaaa                                                                     >  2:95155/1‑148 (MQ=255)
                                                                                gCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCAcc                                                            <  2:62799/149‑1 (MQ=255)
                                                                                     gAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATg                                                       <  1:139872/149‑1 (MQ=255)
                                                                                           tAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGt                                                 >  5:28075/1‑149 (MQ=255)
                                                                                           tAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGt                                                 <  3:294577/149‑1 (MQ=255)
                                                                                                 tAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGTAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTg                                            >  5:229002/1‑148 (MQ=255)
                                                                                                 tAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTAt                                                                                                                 >  5:329493/1‑79 (MQ=255)
                                                                                                 tAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTAt                                                                                                                 <  6:329493/79‑1 (MQ=255)
                                                                                                 tAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATa                                                                       <  7:225638/121‑1 (MQ=255)
                                                                                                 tAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATa                                                                       >  8:225638/1‑121 (MQ=255)
                                                                                                 tAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTg                                            >  3:278811/1‑148 (MQ=255)
                                                                                                     aaCAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATATGCTATCGAAAGTAATTTCTATGACGCATCGTCCTATGCAGGCAAAACCAGTACGCTTCGCATAAAACTGGCACCCGATGGTATGTTACTGGatat                                       >  6:134311/1‑149 (MQ=255)
                                                                                                       cAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATc                                      >  7:51948/1‑148 (MQ=255)
                                                                                                             cgcATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTg                               <  8:338766/149‑1 (MQ=255)
                                                                                                               cATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGt                                                                                   <  1:242436/95‑1 (MQ=255)
                                                                                                               cATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGt                                                                                   >  2:242436/1‑95 (MQ=255)
                                                                                                                aTCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAg                            >  2:29854/1‑149 (MQ=255)
                                                                                                                aTCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAg                            <  8:51948/149‑1 (MQ=255)
                                                                                                                   aGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTg                         >  1:397140/1‑149 (MQ=255)
                                                                                                                             atCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCg                >  5:143674/1‑148 (MQ=255)
                                                                                                                                 aTAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACtt           >  7:102656/1‑149 (MQ=255)
                                                                                                                                  tAACTATGCCGGGTAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACttt          <  6:229002/149‑1 (MQ=255)
                                                                                                                                  tAACTATGCCGGGCAGATTAAATCTGATATCGAAATTAAGTTCTATGACGCATCGTCCTATTGAGGCAAAACATGTACGCTGCGCATAAAACTGGCACCCGATGGTATTTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACttt          <  5:134311/149‑1 (MQ=255)
                                                                                                                                    aCTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTg         <  2:397140/148‑1 (MQ=255)
                                                                                                                                     cTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTAt                                                   >  5:158412/1‑105 (MQ=255)
                                                                                                                                     cTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTAt                                                   <  6:158412/105‑1 (MQ=255)
                                                                                                                                     cTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGt        >  3:143877/1‑148 (MQ=255)
                                                                                                                                      tATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTg                                                                 <  4:141222/90‑1 (MQ=255)
                                                                                                                                      tATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTg                                                                 >  3:141222/1‑90 (MQ=255)
                                                                                                                                      tATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGt        >  7:112784/1‑147 (MQ=255)
                                                                                                                                      tATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGCCACCCGATGGTATGTTACTGGat                                         >  1:317515/1‑114 (MQ=255)
                                                                                                                                      tATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGCCACCCGATGGTATGTTACTGGat                                         <  2:317515/114‑1 (MQ=255)
                                                                                                                                          ccGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCt  >  6:160612/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
ATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCT  >  NC_000913/777225‑777511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: