Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 169,034 C→A 17.7% Y517* (TAC→TAA fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913169,0340CA17.7% 273.0 / 41.7 111Y517* (TAC→TAAfhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (46/45);  new base A (10/10);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 111 total aligned reads displayed.

TTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCC  >  NC_000913/168889‑169180
                                                                                                                                                 |                                                                                                                                                  
ttCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTg                                                                                                                                                 >  6:317290/1‑149 (MQ=255)
ttCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTg                                                                                                                                                 >  2:135175/1‑149 (MQ=255)
ttCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTg                                                                                                                                                 >  2:288889/1‑149 (MQ=255)
  cAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGtt                                                                                                                                               >  3:174090/1‑149 (MQ=255)
   aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGtt                                                                                                                                               <  1:310079/148‑1 (MQ=255)
   aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGttt                                                                                                                                              <  6:154217/149‑1 (MQ=255)
    ggATCAGGCGCAGTGGTATAAAGTGCTGGTAACCCTATGCGGGCGTTATGACTTGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGTACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTg                                                                                                                                             <  3:175196/149‑1 (MQ=255)
     gATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGa                                                                                                                                            <  4:174090/149‑1 (MQ=255)
       tCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGAt                                                                                                                                           >  4:178448/1‑148 (MQ=255)
       tCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATa                                                                                                                                          <  2:27205/149‑1 (MQ=255)
           gcgcAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATg                                                                                                                                       <  4:355035/148‑1 (MQ=255)
                gTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTaa                                                                                                                                 <  1:135175/149‑1 (MQ=255)
                    gATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATg                                                                                                                                       <  5:28515/139‑1 (MQ=255)
                    gATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATg                                                                                                                                       >  6:28515/1‑139 (MQ=255)
                      tAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCt                                                                                                                            >  4:379700/1‑148 (MQ=255)
                        aaGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTa                                                                                                                          <  5:304404/148‑1 (MQ=255)
                        aaGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTAc                                                                                                                         >  6:1279/1‑149 (MQ=255)
                           tGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTc                                                                                                                      <  3:379700/149‑1 (MQ=255)
                                gTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCta                                                                                                                 <  3:235865/149‑1 (MQ=255)
                                gTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGtt                                                                                                                                               >  4:288497/1‑119 (MQ=255)
                                gTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGtt                                                                                                                                               <  3:288497/119‑1 (MQ=255)
                                gTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCta                                                                                                                 >  5:265336/1‑149 (MQ=255)
                                gTCACCCTAGGCGGTCGTGATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGAAGACCGATAAACGTGATGACAAACAGATAACCTAGCGTCCTCGTGTTAACTACCTTATATATAATGGTCTAACACCTTACAACAGCt                                                                                                                  >  2:130542/1‑148 (MQ=255)
                                    cccTAGGAGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGTGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGc                                                                                                             <  1:155894/149‑1 (MQ=255)
                                     ccTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTaa                                                                                                                                 <  4:32782/128‑1 (MQ=255)
                                     ccTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTaa                                                                                                                                 >  3:32782/1‑128 (MQ=255)
                                     ccTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGc                                                                                                             >  4:129029/1‑148 (MQ=255)
                                       tAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGaa                                                                                                          >  8:272618/1‑149 (MQ=255)
                                            ggTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGtt                                                                                                     >  2:382329/1‑149 (MQ=255)
                                                gTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGaa                                                                                                 >  2:296587/1‑149 (MQ=255)
                                                gTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACATGTTTTATAATGGTGCAACACATTAATGCAGATATAGCGAATCGTTTGaa                                                                                                 >  2:394664/1‑149 (MQ=255)
                                                   aTGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGtt                                                                                                     >  7:309529/1‑142 (MQ=255)
                                                   aTGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGtt                                                                                                     <  8:309529/142‑1 (MQ=255)
                                                    tGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACctt                                                                                             >  8:117538/1‑149 (MQ=255)
                                                     gACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACcttc                                                                                            >  1:174280/1‑149 (MQ=255)
                                                     gACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACcttc                                                                                            >  5:263009/1‑149 (MQ=255)
                                                      aCTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACcttct                                                                                           >  7:34367/1‑149 (MQ=255)
                                                       cTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACcttctt                                                                                          >  1:273265/1‑149 (MQ=255)
                                                       cTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCTGCTATAGCGAATCGTTTGAACcttctt                                                                                          <  1:382329/149‑1 (MQ=255)
                                                       cTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACcttctt                                                                                          <  1:294724/149‑1 (MQ=255)
                                                        tGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCttcttc                                                                                         >  4:219267/1‑149 (MQ=255)
                                                               aTCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGtt                                                                                  >  5:209449/1‑149 (MQ=255)
                                                               aTCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATc                                                                                                        <  6:95143/127‑1 (MQ=255)
                                                               aTCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATc                                                                                                        >  5:95143/1‑127 (MQ=255)
                                                                 cAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTgg                                                                                >  2:271724/1‑149 (MQ=255)
                                                                 cAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTgg                                                                                >  1:17474/1‑149 (MQ=255)
                                                                  aaGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTa                                                                                                                                  <  7:181513/98‑1 (MQ=255)
                                                                  aaGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTa                                                                                                                                  >  8:181513/1‑98 (MQ=255)
                                                                   aGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATGATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCATCTTCGCAAGTTggg                                                                               >  7:324068/1‑148 (MQ=255)
                                                                    gAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTACCACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTggg                                                                               >  6:60947/1‑147 (MQ=255)
                                                                    gAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGaa                                                                             <  5:317290/149‑1 (MQ=255)
                                                                    gAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGaa                                                                             <  7:272618/149‑1 (MQ=255)
                                                                         tctTTACCGCGGTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTAGCTAGCGTGGTGGTGTTAACTACCTGTGTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGa                                                                          <  6:286315/147‑1 (MQ=255)
                                                                          ctTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                                       >  7:264662/1‑149 (MQ=255)
                                                                          ctTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                                       >  3:184789/1‑149 (MQ=255)
                                                                           ttAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGa                                                                          <  2:17474/145‑1 (MQ=255)
                                                                             aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGt                                                                      <  3:129029/147‑1 (MQ=255)
                                                                             aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                                       <  5:1279/146‑1 (MQ=255)
                                                                              aCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAat                                                                   <  3:219267/149‑1 (MQ=255)
                                                                                    ttGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTc                                                             <  3:178448/149‑1 (MQ=255)
                                                                                    gttccGGGACGACCGATAATCGTGTTGACAAACAGTTTACCTGGAGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTGGCAAGTTGGGAAGGATGGTAATATTTTc                                                             <  3:351014/146‑1 (MQ=255)
                                                                                           gacgacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGATCCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGt                                                      <  6:183379/149‑1 (MQ=255)
                                                                                           gacgacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAcc                                                                                               <  2:22159/108‑1 (MQ=255)
                                                                                           gacgacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAcc                                                                                               >  1:22159/1‑108 (MQ=255)
                                                                                              gacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCt                                                    >  6:179593/1‑148 (MQ=255)
                                                                                               acCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTa                                                   >  2:244624/1‑148 (MQ=255)
                                                                                                ccGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTaaa                                                 <  1:288889/149‑1 (MQ=255)
                                                                                                 cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGATCCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAg                                                >  4:257886/1‑149 (MQ=255)
                                                                                                  gATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGtt                                                                                  >  8:247482/1‑114 (MQ=255)
                                                                                                  gATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGtt                                                                                  <  7:247482/114‑1 (MQ=255)
                                                                                                       aCGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAg                                           >  6:289/1‑148 (MQ=255)
                                                                                                        cGTGATGACAAACAGTTAACCGAGCGTGGGTGTGTTAACTACCTGTTTGATTATGGTGTAACACCTTACTTCAGCTATAGGGAATCGTTTGAACCTTCATCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCa                                         <  6:4685/149‑1 (MQ=255)
                                                                                                          tgatgaCAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGt                                       <  6:209449/149‑1 (MQ=255)
                                                                                                             tgaCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATg                                    <  6:296776/149‑1 (MQ=255)
                                                                                                             tgaCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATg                                    <  3:221860/149‑1 (MQ=255)
                                                                                                                cAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAg                                 >  4:199587/1‑149 (MQ=255)
                                                                                                                 aaaCAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGt                                >  2:164438/1‑149 (MQ=255)
                                                                                                                  aaCAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGt                                                                                                      <  1:84299/78‑1 (MQ=255)
                                                                                                                     aGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGc                            <  8:219718/149‑1 (MQ=255)
                                                                                                                      gTTTACATAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCg                           <  6:263009/149‑1 (MQ=255)
                                                                                                                       tttACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGt                          >  3:112486/1‑149 (MQ=255)
                                                                                                                             tagCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATAtttt                                                              >  5:259100/3‑107 (MQ=255)
                                                                                                                                cGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGt                 <  5:289/149‑1 (MQ=255)
                                                                                                                                 gtggtggtgTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATg                  >  7:391016/1‑147 (MQ=255)
                                                                                                                                 gtggtggtgTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATg                  <  8:391016/147‑1 (MQ=255)
                                                                                                                                  tgggggggTTTACTACCTGTTTGTTAATGGTGTTACACCTTACTTCAGCTATTGCGAATCGTTTGTACCTTCTTCGCAATTTGGGCAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTAc               <  8:264662/149‑1 (MQ=255)
                                                                                                                                    gtggtgTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCg             <  1:271724/149‑1 (MQ=255)
                                                                                                                                          ttAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTaaa                                                 >  6:235594/1‑107 (MQ=255)
                                                                                                                                          ttAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTaaa                                                 <  5:235594/107‑1 (MQ=255)
                                                                                                                                          ttAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTa                                                   <  2:355856/105‑1 (MQ=255)
                                                                                                                                          ttAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTa                                                   >  1:355856/1‑105 (MQ=255)
                                                                                                                                            aaCTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCg                                                                                                       >  4:276814/1‑51 (MQ=255)
                                                                                                                                            aaCTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCg                                                                                                       <  3:276814/51‑1 (MQ=255)
                                                                                                                                             aCTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAata                    >  1:207422/1‑133 (MQ=255)
                                                                                                                                              cTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGACTCGTTTGAACCTTCTTCGCAAGTTGGTAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGAt       >  3:7664/1‑145 (MQ=255)
                                                                                                                                              cTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGAt       <  4:2371/145‑1 (MQ=255)
                                                                                                                                              cTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTc   <  7:345225/149‑1 (MQ=255)
                                                                                                                                              cTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTc   >  8:345225/1‑149 (MQ=255)
                                                                                                                                              cTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGAGAATCGTTTGAACCTTCTTCGCAAGTTGTGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGAt       <  4:7664/145‑1 (MQ=255)
                                                                                                                                               tACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTcc  >  1:99200/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCC  >  NC_000913/168889‑169180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: