Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,510,710 G→A 5.9% Q236Q (CAG→CAA ydcR → putative DNA‑binding transcriptional regulator and putative aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,510,7100GA 5.9% 220.1 / 2.9 69Q236Q (CAG→CAAydcRputative DNA‑binding transcriptional regulator and putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (27/37);  new base A (2/2);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TAACTGAACCGGGCGATTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTT  >  NC_000913/1510562‑1510857
                                                                                                                                                    |                                                                                                                                                   
tAACTGAACCGGGCGATTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAg                                                                                                                                                     <  3:152385/149‑1 (MQ=255)
 aaCTGAACCGGGCGATTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAg                                                                                                                                                     >  3:35549/1‑148 (MQ=255)
      aaCCGGGCGATTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGAACTGGCGTTGCAGGAGTATc                                                                                                                                              <  3:45700/149‑1 (MQ=255)
       aCCGGGCGATTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATc                                                                                                                                              <  1:305961/149‑1 (MQ=255)
         cGGGCGATTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCg                                                                                                                                            <  7:75891/149‑1 (MQ=255)
          gggCGTTTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCgg                                                                                                                                           <  1:127545/149‑1 (MQ=255)
           tgtgATTGGGTTATAGTAGATAATCATTGTTTCTACGGTGCGTTTCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTTTCGGTGGCGTCGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGt                                                                                                                                          <  4:14685/146‑1 (MQ=255)
               aTTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGaaa                                                                                                                                      <  7:46953/149‑1 (MQ=255)
                  gggTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGc                                                                                                                                    <  8:275222/148‑1 (MQ=255)
                   ggTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGt                                                                                                                                  <  4:35549/149‑1 (MQ=255)
                   ggTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGt                                                                                                                                  >  4:334937/1‑149 (MQ=255)
                    gTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTg                                                                                                                                 <  8:85660/149‑1 (MQ=255)
                       aTAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTAGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTgctg                                                                                                                              <  7:33371/149‑1 (MQ=255)
                         agtagAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTgctggc                                                                                                                            >  7:27891/1‑149 (MQ=255)
                          gtagAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTgctggc                                                                                                                            <  6:205926/148‑1 (MQ=255)
                             gagaATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCtgat                                                                                                                        >  8:46549/1‑149 (MQ=255)
                             gagaATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCtgat                                                                                                                        <  8:27891/149‑1 (MQ=255)
                             gagaATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCtgat                                                                                                                        >  7:259450/1‑149 (MQ=255)
                             gagaATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCtgat                                                                                                                        <  1:214137/149‑1 (MQ=255)
                                  tCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTa                                                                                                                   <  2:238764/149‑1 (MQ=255)
                                     ttGTTTCTACTGTTCTTTGAAGGCTCTGTAGCGGCTACGGCTGAAGTCGTTATCGGTGGCGACTGATGTAAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCtga                                                                                                                         <  4:41740/140‑1 (MQ=255)
                                     ttGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATGCGGTGAAAGCGTgctggctgc                                                                                                                         >  3:41740/1‑139 (MQ=255)
                                     ttGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTaa                                                                                                                  >  8:164136/1‑147 (MQ=255)
                                            tACGGTGCGTTGCAGGCGCTGGAGTGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGaa                                                                                                         >  2:175724/1‑149 (MQ=255)
                                              cGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATc                                                                                                       <  3:334937/149‑1 (MQ=255)
                                                 tGCGTTGCAGGCGCTGGAGTGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCAc                                                                                                    <  1:175724/149‑1 (MQ=255)
                                                     ttGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCgg                                                                                                <  7:164136/149‑1 (MQ=255)
                                                        cAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCATGAGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCAATCGGAtt                                                                                             >  7:127535/1‑149 (MQ=255)
                                                        cAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGAtt                                                                                             >  7:133430/1‑149 (MQ=255)
                                                             gcTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCt                                                                                        >  8:299576/1‑149 (MQ=255)
                                                                     ggCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCt                                                                                        >  4:372548/1‑141 (MQ=255)
                                                                     ggCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCt                                                                                        <  3:372548/141‑1 (MQ=255)
                                                                                taGGCGTTATCGGTGTCGTAGGATGTTTATGAATGGATAGATCTTCAGGCGCTTGAACTGGCGTTGGAGGATTATCCTGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCTCTCGGATTTACCTTATCGCCGCaaaaaaatg                                                                      <  8:133430/147‑3 (MQ=255)
                                                                                aaGGCGTTATCGTTGTCGAGTGATTTTATTGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGGAGGAGTATCCTTTGAAAGCGTGTTGGCTGATGACTAATAGAAAGAATCCTCTCTGATTTACCTTAACGCCGCAAAAAAAAg                                                                      <  8:127535/148‑1 (MQ=255)
                                                                                aaGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCaaaaaaaa                                                                       <  7:93679/147‑1 (MQ=255)
                                                                                aaGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCaaaaaaaa                                                                       <  8:259450/147‑1 (MQ=255)
                                                                                aaGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCaaaaaaaa                                                                       <  6:268132/147‑1 (MQ=255)
                                                                                aaGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCaaaaaaaa                                                                       <  7:20233/147‑1 (MQ=255)
                                                                                                 gACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGc                                                    >  3:175716/1‑149 (MQ=255)
                                                                                                 gACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGc                                                    <  7:154216/149‑1 (MQ=255)
                                                                                                 gACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGc                                                    >  6:106806/1‑149 (MQ=255)
                                                                                                 gACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGc                                                    >  8:12337/1‑149 (MQ=255)
                                                                                                 gACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGc                                                    <  7:299576/149‑1 (MQ=255)
                                                                                                 gACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCACGAGTAACAGGTGAAAGCGTGCTGCCTGATGACTAATATCCAGAATCCACTCGGATTTACCTTAACGACGCAAAAAAAAGAGCAACTGGTGGCGTTGc                                                    >  2:341392/1‑149 (MQ=255)
                                                                                                     gATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCaa                                                <  2:98063/149‑1 (MQ=255)
                                                                                                      aTGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGGGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAATAAGCACAACTGGTGGCGTTGCTCAAt                                               <  4:175716/149‑1 (MQ=255)
                                                                                                      aTGTTAAAGAAGGGATACTTCTTCAGGCGCTGGAACTTGCGGTGCAGGAGTATCCGGTTAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                               <  7:322578/149‑1 (MQ=255)
                                                                                                          tAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAg                                            >  3:369263/1‑148 (MQ=255)
                                                                                                           aaaGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGt                                           >  6:141283/1‑148 (MQ=255)
                                                                                                              gaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAAGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGt                                                       >  3:158942/1‑133 (MQ=255)
                                                                                                              gaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAAGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGt                                                       <  4:153272/133‑1 (MQ=255)
                                                                                                              gaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAAGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGt                                                       <  4:158942/133‑1 (MQ=255)
                                                                                                              gaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAAGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGt                                                       >  3:153272/1‑133 (MQ=255)
                                                                                                                   gATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTaa                                  >  2:335573/1‑149 (MQ=255)
                                                                                                                     tAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCTGGACGACGTATCg                                >  2:410129/1‑149 (MQ=255)
                                                                                                                        aTCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCt                              >  3:7307/1‑148 (MQ=255)
                                                                                                                        aTCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGCTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCt                              >  6:51336/1‑148 (MQ=255)
                                                                                                                           ttCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGAtt                          >  1:58921/1‑149 (MQ=255)
                                                                                                                           ttCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGAtt                          >  8:178079/1‑149 (MQ=255)
                                                                                                                             cAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGAtt                          <  7:12337/147‑1 (MQ=255)
                                                                                                                             cAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGAt                           <  7:46549/146‑1 (MQ=255)
                                                                                                                              aGGCTCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGa                        <  1:341392/148‑1 (MQ=255)
                                                                                                                              aGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGaa                       <  5:106806/149‑1 (MQ=255)
                                                                                                                                      gAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGt               >  7:400198/1‑149 (MQ=255)
                                                                                                                                       aaCTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACtg                                                              <  7:296960/101‑1 (MQ=255)
                                                                                                                                       aaCTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACtg                                                              >  8:296960/1‑101 (MQ=255)
                                                                                                                                         cTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCaaaaaaaa                                                                       <  6:185462/90‑1 (MQ=255)
                                                                                                                                         cTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACATTAACGCCGCaaaaaaaa                                                                       >  5:185462/1‑90 (MQ=255)
                                                                                                                                            gCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGaa                       >  4:307112/1‑135 (MQ=255)
                                                                                                                                            gCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGaa                       <  3:307112/135‑1 (MQ=255)
                                                                                                                                                   aGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGTCGTTGCTCAATCAGTACAACGTAACGCTGATTGAATATGACTATTACAGCGAACtt  >  6:259799/1‑149 (MQ=255)
                                                                                                                                                    ggAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTa                                          <  2:123364/108‑1 (MQ=255)
                                                                                                                                                    ggAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTa                                          >  1:123364/1‑108 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TAACTGAACCGGGCGATTGGGTGATAGTAGAGAATCCTTGTTTCTACGGTGCGTTGCAGGCGCTGGAGCGGCTACGGCTGAAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTT  >  NC_000913/1510562‑1510857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: