| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,614,396 | T→C | 6.9% | H103R (CAT→CGT) | sad ← | succinate semialdehyde dehydrogenase, NAD(P)+‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,614,396 | 0 | T | C | 6.9% | 175.1 / 3.7 | 58 | H103R (CAT→CGT) | sad | succinate semialdehyde dehydrogenase, NAD(P)+‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (32/22); new base C (2/2); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01 | |||||||||||
ATGATGGGAACAGCGCCACGCATCACCTGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAGC > NC_000913/1614252‑1614544 | atgatgGGAACAGCGCCACGCATCACCTGCCATAACGGAAAATTCCAATGCATAATCGCCAGAATCGTCCACAACGGTCGATACTCAATAAACGCCTGCTGATTTTCCACCCGCGTAGGTTCCGCCTTCAGCATTGCCTGACAATgat > 2:245107/1‑146 (MQ=255) ggAACAGCGCCACGCATCACCTGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTc > 4:88968/1‑143 (MQ=255) ggAACAGCGCCACGCATCACCTGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTc < 3:88968/143‑1 (MQ=255) gAACCGCGACACCCATCACCTGCCATAACGTAAAATTCCACGGCATAATCGCCAGACTCGGCCCCTACGGGCGATACTCAAGAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATAc < 4:310211/149‑1 (MQ=255) aGCGCCACGCATCACCTGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGt < 3:126967/149‑1 (MQ=255) tCACCTGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTc < 8:257481/149‑1 (MQ=255) cTGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGcc > 6:146799/1‑148 (MQ=255) cTGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGATCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGcc > 1:284824/1‑148 (MQ=255) tGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGCTACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGa > 2:213528/1‑149 (MQ=255) cGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCa > 4:305853/1‑149 (MQ=255) cGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCa < 2:284824/149‑1 (MQ=255) gAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTc < 5:260415/134‑1 (MQ=255) gAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTc > 6:260415/1‑134 (MQ=255) aaTTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCa < 3:305853/149‑1 (MQ=255) tCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACGCAAAACCCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACGAGTCCCACAAATTCGCCGATTTCGCCACTTCAgcg > 4:298875/1‑149 (MQ=255) aTCGCCAGAATCGTCCCCAAGGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCATAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGtt > 2:271655/1‑149 (MQ=255) aTCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGtt < 5:146799/149‑1 (MQ=255) aTCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGtt < 4:148111/149‑1 (MQ=255) aTCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGtt < 8:21078/149‑1 (MQ=255) gCCAGAATCGTCCCCAATGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCCTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGAt < 1:271655/149‑1 (MQ=255) gCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGAt > 5:295577/1‑149 (MQ=255) cccAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCACGTTCTGCATACCAGTCACACAAATTCGCCGAt < 6:210289/108‑1 (MQ=255) cccAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCACGTTCTGCATACCAGTCACACAAATTCGCCGAt > 5:205150/1‑108 (MQ=255) cccAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCACGTTCTGCATACCAGTCACACAAATTCGCCGAt > 5:210289/1‑108 (MQ=255) cccAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCACGTTCTGCATACCAGTCACACAAATTCGCCGAt < 6:205150/108‑1 (MQ=255) cAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCg > 5:220767/1‑101 (MQ=255) cAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCg < 6:220767/101‑1 (MQ=255) gTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATACGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCgg > 2:80325/1‑149 (MQ=255) aTACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGa > 5:56245/1‑149 (MQ=255) tCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACGATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGt < 8:385638/118‑1 (MQ=255) tCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGt > 7:385638/1‑118 (MQ=255) tCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCAt > 4:148168/1‑149 (MQ=255) tAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGCCCATGTTCTGCATACCAGTCACACAAATTCGCCCATTTCGCCACTTCAGCGCGCGCCTGGTTGATAGCTTTGCCCATTTCGCGGGTGATCATTTgc > 3:35253/1‑149 (MQ=255) ccGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCCGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCACATTTCGAGGGTGATCATTTgcgc > 4:203722/1‑148 (MQ=255) ccGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTTCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTgcgc > 1:85105/1‑148 (MQ=255) ctgctgATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACTATGTTCTGCATACCAGTCACACAAAATCGCCGATTTCGCCACTTCAGCGCGCGCCTGGATGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCAttt > 4:339925/1‑149 (MQ=255) ctgctgATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCCGCGCGCGCCTGGTTGATTGGTTTGCCCATTACGCTGGTGATCATTTGCGCCAtat > 2:162067/1‑147 (MQ=255) accaGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAgc > 1:242412/1‑149 (MQ=255) accaGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAgc < 7:326924/149‑1 (MQ=255) caGCTTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAgcgc < 1:162067/149‑1 (MQ=255) gTAGGTTCCGCCTTCAGCATTGCCGGACCATGGTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAgcgcg > 2:287118/1‑146 (MQ=255) aGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCagag > 2:313354/1‑149 (MQ=255) ggTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGtt < 8:646/94‑1 (MQ=255) ggTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGtt > 7:646/1‑94 (MQ=255) ccGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGTTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCtt > 5:81281/1‑148 (MQ=255) cGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTAc > 4:157242/1‑149 (MQ=255) cGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATGTCGCCACTTCAGCGCGCGCCTGGTGGATTGGTGTGCCCATTTCGCGGGGGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTAc < 1:213528/149‑1 (MQ=255) gCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCCGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTccc > 1:206112/1‑149 (MQ=255) ttCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCAGTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGACTTACCGa > 4:194585/1‑148 (MQ=255) cAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGat < 3:148168/147‑1 (MQ=255) aGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATc < 3:157242/149‑1 (MQ=255) aGCATTGCCGGACCATGGTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGatat < 1:287118/148‑1 (MQ=255) ttGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACg > 1:391286/1‑148 (MQ=255) gACCATGTTCTGCATACCAGTCACACAAATTCGCAGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCAATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGtttt > 3:164029/1‑149 (MQ=255) aCCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGttttt > 6:210500/1‑149 (MQ=255) ccATGTTCTGCATACCTGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGAGTGGTTTGCCCATTTCGCGGGTGAGCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTc < 3:203722/149‑1 (MQ=255) ccATGTGCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCTGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTc < 1:245107/149‑1 (MQ=255) cATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGttttt > 6:141146/1‑147 (MQ=255) tGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAg > 5:335260/1‑148 (MQ=255) tGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAGc < 1:313354/149‑1 (MQ=255) | ATGATGGGAACAGCGCCACGCATCACCTGCCATAACGGAAAATTCCACGGCATAATCGCCAGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAGC > NC_000913/1614252‑1614544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |