Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,958 T→C 20.4% V680V (GTT→GTC)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,9580TC20.4% 75.2 / 10.2 46V680V (GTT→GTC) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (21/12);  new base C (2/6);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGG  >  NC_000913/1708814‑1709100
                                                                                                                                                |                                                                                                                                              
aCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCAAGAACATCAGCTCCATCGCCGCAAATCCGAGCTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAt                                                                                                                                             <  8:229900/148‑1 (MQ=17)
             cAGCAACAGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCAAAGCAGCAGTAGAAGCCGCTATTGCCCGAGCAAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAGCAAGAAGAACACGTAGCACCGCGAACAGCtcc                                                                                                                               >  5:183024/1‑147 (MQ=14)
                cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAATCGCGCAAACGGGAACAGCAACCGGCTAATTCTGAGCAAGAAGAACAGGTTGATCcgc                                                                                                                                         >  2:22931/1‑136 (MQ=255)
                cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgc                                                                                                                                         <  1:22931/136‑1 (MQ=255)
                        tAATGCGGATGCAGAACAAGCGTTCCAAGCGCGCCAAGAGGCCGTGGATGGCGCTCTTGCCCGAGCCATAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAACAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCgg                                                                                                                    <  1:291413/149‑1 (MQ=18)
                        tAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAACCACAGGAACAGGTAGATCCGCGCAAACCTGACCTCGaacctg                                                                                                                    >  6:333525/1‑145 (MQ=14)
                            gCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTAt                                                                                                                >  8:71556/1‑149 (MQ=255)
                               gAGCCAGAACAACATGTCGATCCGCTCAACGCCGCCGACGAAGCCGCTATTGCCCGAGCCACAGCGCCCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGc                                                                                                             <  1:51189/149‑1 (MQ=14)
                               gAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGACCCGAGGCGCGCAGACAGGAGAAGAGACCGCCTCAGGCGTCGCCAGAAGAGGAGGTAGAACAGCGCAAAGAGGCCGTCGCAGCGGcggttgg                                                                                                             >  8:108616/1‑143 (MQ=9)
                                   cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCAGCTATTGCAcgc                                                                                                         <  7:71556/149‑1 (MQ=255)
                                   cAGAACAACAGGGCGATCCGCGCAAAGCCCCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGGAACCGGCTAATGCGCAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcgc                                                                                                         <  5:348205/149‑1 (MQ=14)
                                             tcgcgagccgagcACAGCCGCCGGCGCATCCGCTCTTGCCCGAGCCAAAGCCCGCAACCTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCAc                                                                                               <  5:276493/146‑1 (MQ=18)
                                                                      gAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGACCAGCAACAGGCTaa                                                                      >  5:389157/1‑149 (MQ=255)
                                                                           cGCTATTGTCCTCTCCAAAGCGCGCAATCGGGATCATCATCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCgg                                                                 <  5:333525/149‑1 (MQ=17)
                                                                            gCTAGTGCCCGAGCCAAAGCTCGCAAACTGTAACATCAACAGGCTAATGCGGAGCCAGAACAACAGGTTGATCCGCGCAATGCTTCCTTCGAAGCGTCTATTGCTCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                                                                <  8:57354/149‑1 (MQ=14)
                                                                                   cccGAGCCAAAGCGCGCAAACGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCCACGCTGCCGGCGAAGAGGCTATTGCACGCGCCCAATCCCGCAAGCTGGATCAGCAACAGGCTAcggcggggcccg                                                           >  1:251418/1‑141 (MQ=25)
                                                                                    ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCAAAAGCAAGCAAGCTCGAACAGCAACAGGCTAATGCCGTACCag                                                           >  1:64182/1‑146 (MQ=17)
                                                                                    ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGAAGCGGCTATTGCCCGCGCCAACGCACGCAAGCTGGCGCAGCAGCAGGCTACTGCGGTACCag                                                           >  1:48446/1‑146 (MQ=21)
                                                                                     cGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcgc                                                                                                         <  6:355175/99‑1 (MQ=255)
                                                                                     cGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcgc                                                                                                         >  5:355175/1‑99 (MQ=255)
                                                                                           aaaGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGAACACGCTAGAGCACCCGACAGAGCACCCATCCGGGAACCGCAACAGGCTAAACCTGTACCAGAAGAACaac                                                  >  5:14536/1‑146 (MQ=18)
                                                                                                 cgcAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAg                                                                                          <  1:316310/102‑1 (MQ=255)
                                                                                                 cgcAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAg                                                                                          >  2:316310/1‑102 (MQ=255)
                                                                                                    aaaCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGCCGCGGCTATTGCCCGCGCCAAGGCACAAAAGAAGCACCAGGGACAGGCAAGAGGGTTAACAGAAGAGCAGGTAGCTCcgcg                                        >  2:204420/1‑149 (MQ=9)
                                                                                                           aaCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGCAGCGGCTATTGCACGCGCAAAAGCACAAAAGCAGGCACAGCAGCAGGATATTGAGGAACAAGAAGATCAGGTAGATCCTCGCAAAGCg                                 >  2:150363/1‑149 (MQ=18)
                                                                                                                cAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAACGCACGCAAGCTGGAACTGCAACAGGCTAATGCTGTACCAGAAGACCAGGGTGCTCCGCGCAAAGCGGCAGt                            >  3:238671/1‑149 (MQ=38)
                                                                                                                 aaCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAg                                                                                 >  4:162683/1‑95 (MQ=255)
                                                                                                                 aaCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAg                                                                                 <  3:162683/95‑1 (MQ=255)
                                                                                                                 aaCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGtt                           >  8:70202/1‑149 (MQ=255)
                                                                                                                      gCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGc                                                                                                             <  2:287428/62‑1 (MQ=255)
                                                                                                                           tGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGc                                                                                         <  2:315577/77‑1 (MQ=255)
                                                                                                                            gCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaag                                                        >  8:255520/1‑109 (MQ=255)
                                                                                                                            gCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaag                                                        <  7:255520/109‑1 (MQ=255)
                                                                                                                               gAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACa                                                                                  >  4:68380/1‑80 (MQ=14)
                                                                                                                                 gCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGccc           >  3:379942/1‑149 (MQ=255)
                                                                                                                                 cgctctagaaCCGGTGGATCCGCGAAAATCTTCCGTCGAAGCGGCTATTGCACGCGCAAAAGCACGCAATCTGGAACACCAACAGGCTACTGCTGGACCAGTAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGc             <  6:25773/141‑1 (MQ=18)
                                                                                                                                    agaagaACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTCCCAGAAGAACAGGTTGCTCCGCGCAAACCGGCAGTTGCCGCGGCTATTGCCcgcg        >  5:311697/1‑149 (MQ=255)
                                                                                                                                    agaagaACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGATATTGCCcgcg        >  6:153301/1‑149 (MQ=255)
                                                                                                                                    agaagaACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaaa                                                                                                   <  6:84671/58‑1 (MQ=255)
                                                                                                                                     gaagaaCAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg                                                                                                              <  6:133754/46‑1 (MQ=255)
                                                                                                                                        gaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCcgatcgg     >  5:25773/1‑143 (MQ=255)
                                                                                                                                         aaCAGGTTGATCCGCGCAAAGCTGCAGTAGAAGCGGCTATTGCACGCGCAAAAGCACGAAAGCACGAACAGCAACAGGCTAAAGAGGTACCATAAGAACAGGTAGATCCGCGCAAAGCTGCTGATGCCGCGGCTATTGCCCGCGCTCAg   >  6:200581/1‑149 (MQ=255)
                                                                                                                                         aaCAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAgg                                                                          <  1:266471/78‑1 (MQ=255)
                                                                                                                                         aaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAgg                                                                          >  2:266471/1‑78 (MQ=255)
                                                                                                                                          accggTAGATCCGCGCCACCCTCCCGTCGACCCGGCTATTGCTCGCGCCACCGCACGCAACCGGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAgg  <  1:204420/146‑1 (MQ=18)
                                                                                                                                          aCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAgg  >  3:8820/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
ACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGG  >  NC_000913/1708814‑1709100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑