Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,744,575 T→C 5.3% I373I (ATT→ATC mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,5750TC 5.3% 233.5 / 2.6 76I373I (ATT→ATCmdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base T (37/34);  new base C (2/2);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

ATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTG  >  NC_000913/1744429‑1744721
                                                                                                                                                  |                                                                                                                                                  
ctGGCAACCCTGACGGCCATTTTCTCGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGATATATCAGATTTCTGACTCAATCCAGGTGATTgg                                                                                                                                                  <  6:76349/148‑1 (MQ=255)
 tGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTgg                                                                                                                                                  <  1:294229/148‑1 (MQ=255)
        ccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGa                                                                                                                                          >  5:31741/1‑149 (MQ=255)
        ccTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGATGTACATCGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGa                                                                                                                                          >  5:373336/1‑149 (MQ=255)
          tGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGAtt                                                                                                                                        >  6:245717/1‑149 (MQ=255)
          tGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGAtt                                                                                                                                        >  7:10015/1‑149 (MQ=255)
             cGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTg                                                                                                                                     >  3:279182/1‑149 (MQ=255)
                ccATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGt                                                                                                                                  >  2:60607/1‑149 (MQ=255)
                  aTTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGGATATCAGATTTCTGACTCAAGCCAGGTGATTGGCAGTGGGATTTTGCGTgg                                                                                                                                >  8:357487/1‑149 (MQ=255)
                   ttttCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGa                                                                                                                                          >  4:215040/1‑138 (MQ=255)
                   ttttCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGa                                                                                                                                          >  4:209835/1‑138 (MQ=255)
                   ttttCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGa                                                                                                                                          <  3:215040/138‑1 (MQ=255)
                   ttttCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGa                                                                                                                                          <  3:209835/138‑1 (MQ=255)
                        aCGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTtata                                                                                                                           <  7:227515/148‑1 (MQ=255)
                         cGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATaaa                                                                                                                         <  1:60607/149‑1 (MQ=255)
                                  tGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATGGGCAGTGGGCTTTTGCGTGGTTATAAAGCTAcgagg                                                                                                                >  8:285648/1‑146 (MQ=255)
                                     gggAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTAAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTc                                                                                                              >  5:358002/1‑148 (MQ=255)
                                       gAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGAtttt                                                                                                                                      <  1:361765/122‑1 (MQ=255)
                                       gAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGAtatt                                                                                                                                      >  2:361765/1‑122 (MQ=255)
                                            aaTCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTc                                                                                                       >  7:219086/1‑148 (MQ=255)
                                              tCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCtat                                                                                                    <  8:10015/149‑1 (MQ=255)
                                                 cccTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTatat                                                                                                  >  8:366394/1‑148 (MQ=255)
                                                        gtACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTa                                                                                          >  3:195185/1‑149 (MQ=255)
                                                         tACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTAc                                                                                         >  5:352949/1‑149 (MQ=255)
                                                           cAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACgg                                                                                       <  5:245717/149‑1 (MQ=255)
                                                            aaCGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGc                                                                                      <  6:31741/149‑1 (MQ=255)
                                                            aaCGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGc                                                                                      >  3:341109/1‑149 (MQ=255)
                                                                 cAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACt                                                                                 >  3:276300/1‑149 (MQ=255)
                                                                   aTCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTgg                                                                               <  6:352949/149‑1 (MQ=255)
                                                                    tCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTggg                                                                              >  3:187515/1‑149 (MQ=255)
                                                                      ccGAGGTTGTAACGCTGGCTGCGGATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTgg                                                                               <  5:132706/146‑1 (MQ=255)
                                                                      ccGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTgg                                                                               >  6:132706/1‑146 (MQ=255)
                                                                       cGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGc                                                                           >  7:185162/1‑149 (MQ=255)
                                                                        gAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGTTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCt                                                                          >  1:315413/1‑149 (MQ=255)
                                                                        gAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCt                                                                          <  6:373336/149‑1 (MQ=255)
                                                                         aGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATGCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTg                                                                         <  4:187515/149‑1 (MQ=255)
                                                                         aGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTg                                                                         >  3:279842/1‑149 (MQ=255)
                                                                              gTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCtt                                                                    >  4:299837/1‑149 (MQ=255)
                                                                                       gctgCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGTTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCgg                                                           <  2:315413/149‑1 (MQ=255)
                                                                                       gctgCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGc                                                             <  8:217694/147‑1 (MQ=255)
                                                                                       gctgCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCgg                                                           <  3:299837/149‑1 (MQ=255)
                                                                                         tgCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTa                                                                                   <  3:195227/123‑1 (MQ=255)
                                                                                         tgCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTa                                                                                   >  4:195227/1‑123 (MQ=255)
                                                                                           cgcATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCta                                                        <  8:185162/148‑1 (MQ=255)
                                                                                            gcATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCtata                                                      >  4:28789/1‑149 (MQ=255)
                                                                                              aTTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCTTGGTTATAAAGATACCCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTTGGCTTGCCAAGCGGCTATAtt                                                    >  4:292502/1‑149 (MQ=255)
                                                                                              aTTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATAtt                                                    <  7:366394/149‑1 (MQ=255)
                                                                                              aTTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTCGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTa                                                                                          <  4:110238/111‑1 (MQ=255)
                                                                                              aTTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATCGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATAtt                                                    >  7:4631/1‑149 (MQ=255)
                                                                                               tttGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCTGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTc                                                   >  2:77058/1‑149 (MQ=255)
                                                                                               tttGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTc                                                   >  4:325740/1‑149 (MQ=255)
                                                                                               tttGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATCGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTc                                                   <  8:4631/149‑1 (MQ=255)
                                                                                                  gATGTTGCTGGCGGCGGTATATCTGATTTGTGACTCTATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATATAGATACGCGTTCCATTTTGTATATTACCTTTACGGCTTAGTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTg                                                 <  4:276300/148‑1 (MQ=255)
                                                                                                  gATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTa                                                                                          >  6:10392/1‑107 (MQ=255)
                                                                                                  gATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTa                                                                                          <  5:10392/107‑1 (MQ=255)
                                                                                                  gATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTg                                                 <  8:254714/148‑1 (MQ=255)
                                                                                                  gATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTg                                                 >  7:254714/1‑148 (MQ=255)
                                                                                                  gATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTg                                                 <  4:279182/148‑1 (MQ=255)
                                                                                                          tgcaggcggTATAACAGATTTCTGACTAAATCCTGGAGATTGGCAGTGGGATTTTGAGAGGTTATAAAGATACGGGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGAcc                                        >  6:198371/5‑149 (MQ=255)
                                                                                                             cggcggTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGAt                                     <  4:195185/149‑1 (MQ=255)
                                                                                                                       tCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTAATAAAGATACGCGTTCCATTTTCTATATTACGAGTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCAGTGACCGATCTGGTCGtt                            >  2:321401/1‑148 (MQ=255)
                                                                                                                       tCAGATTTCTGACTCAATCCAGGTGATCGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGtt                            >  2:261438/1‑148 (MQ=255)
                                                                                                                         aGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTTCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGaa                         <  8:219086/149‑1 (MQ=255)
                                                                                                                               cTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTAt                    <  5:73068/148‑1 (MQ=255)
                                                                                                                                tGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACt                                            <  1:259743/123‑1 (MQ=255)
                                                                                                                                tGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACt                                            >  2:259743/1‑123 (MQ=255)
                                                                                                                                    tCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTgg                                 >  1:335798/1‑130 (MQ=255)
                                                                                                                                    tCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTgg                                 <  2:335798/130‑1 (MQ=255)
                                                                                                                                    tCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATggg                 >  1:257089/1‑146 (MQ=255)
                                                                                                                                    tCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATggg                 <  2:257089/146‑1 (MQ=255)
                                                                                                                                      aagccAGGTGATTGGCAGTGGGATTTTGAGTGGTTATAACGATACGCTGTCAATTTTCTATATTACCTTTACGGCTTACTGTGTGAGGGGTTTGCCAAGCGGCTATATTCTTGCACTGACCGAGCTGGTCGTTGAACCTCTGGGGCcag            <  7:285648/146‑1 (MQ=255)
                                                                                                                                       aTCCAGGTGATCGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCcagc           <  1:261438/149‑1 (MQ=255)
                                                                                                                                        tCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCcagca          <  6:358002/149‑1 (MQ=255)
                                                                                                                                          cAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGAcc                                        >  7:265655/1‑117 (MQ=255)
                                                                                                                                          cAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGAcc                                        <  8:265655/117‑1 (MQ=255)
                                                                                                                                                aTTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTg  >  4:76100/1‑149 (MQ=255)
                                                                                                                                                 ttGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTg  >  3:145837/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAATCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTG  >  NC_000913/1744429‑1744721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: