Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,805,405 T→C 5.3% T227A (ACA→GCA)  ydiY ← acid‑inducible putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,805,4050TC 5.3% 244.4 / 3.3 78T227A (ACA→GCA) ydiYacid‑inducible putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (33/39);  new base C (2/2);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

AATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAACTGCCATGCATAAGCCCC  >  NC_000913/1805257‑1805552
                                                                                                                                                    |                                                                                                                                                   
aaTGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCAtgt                                                                                                                                                     >  7:204850/1‑149 (MQ=255)
   gATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGAc                                                                                                                                                  >  2:236636/1‑149 (MQ=255)
   gATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGAc                                                                                                                                                  >  2:8218/1‑149 (MQ=255)
        ggggCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTg                                                                                                                                              <  5:82481/148‑1 (MQ=255)
         gggCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGt                                                                                                                                             >  6:226207/1‑148 (MQ=255)
             tGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGc                                                                                                                                         >  6:147430/1‑148 (MQ=255)
             tGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGCGACGtt                                                                                                                                               <  4:181971/142‑1 (MQ=255)
             tGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGCGACGtt                                                                                                                                               <  4:176134/142‑1 (MQ=255)
             tGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGCGACGtt                                                                                                                                               >  3:181971/1‑142 (MQ=255)
             tGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGCGACGtt                                                                                                                                               >  3:176134/1‑142 (MQ=255)
             ggAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGcc                                                                                                                                        <  8:144212/148‑1 (MQ=255)
               tgCAGTGAGAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCa                                                                                                                                       <  3:14514/147‑1 (MQ=255)
               aGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGc                                                                                                                                         <  5:147430/146‑1 (MQ=255)
                  aGTGACAAACGAAGACAGCCAGACCTCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCtt                                                                                                                                   <  7:373414/149‑1 (MQ=255)
                   gTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATTAGTTTACATGCTGTTGCCCAGCGATAAGGTTGTCCGCCGGTCGGTATGTTCCGGTGCAGACGCTGGAGGTTCAGAGTTCCATGTGCCGGTGTAAGCCCCCTTc                                                                                                                                  >  4:343509/1‑149 (MQ=255)
                   gTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGAAAATTTTACATGCAGTAGCCCAGCGATATGGTTGTCCGACGGTCGGTAAGTTACGGTGCAGACTCTGCAGGTTCAGAGTTCCATGAGACGTTGTAAGCCAcaaa                                                                                                                                   >  4:77599/1‑145 (MQ=255)
                    tGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCa                                                                                                                                 <  7:221577/149‑1 (MQ=255)
                    tGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCa                                                                                                                                 <  4:267739/149‑1 (MQ=255)
                     gACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTaa                                                                                                                                           >  3:68724/1‑138 (MQ=255)
                     gACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTaa                                                                                                                                           <  4:68724/138‑1 (MQ=255)
                     gACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAg                                                                                                                                >  8:280982/1‑149 (MQ=255)
                     gACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAg                                                                                                                                >  7:187618/1‑149 (MQ=255)
                       cAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTc                                                                                                                              >  7:414643/1‑149 (MQ=255)
                          aCGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCa                                                                                                                            >  3:169308/1‑148 (MQ=255)
                              aGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATg                                                                                                                       <  8:50979/149‑1 (MQ=255)
                                  aGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAAt                                                                                                                        >  5:183673/1‑144 (MQ=255)
                                  aGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAAt                                                                                                                        <  6:183673/144‑1 (MQ=255)
                                   gCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCAt                                                                                                                  <  8:204850/149‑1 (MQ=255)
                                    ccAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCTcc                                                                                                                                     <  6:181277/129‑1 (MQ=255)
                                       gACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTaa                                                                                                               >  3:230669/1‑148 (MQ=255)
                                        aCCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCGAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGCTGCAGACTCTGGACGTTCAGAGTTCCATCTGACGTTCTAAGCAACCTTCAGTCCAAAATGTTCATTaa                                                                                                               >  6:101279/1‑147 (MQ=255)
                                          cGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAg                                                                                                            >  1:218159/1‑148 (MQ=255)
                                            cATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCaa                                                                                                         >  2:30048/1‑149 (MQ=255)
                                             aTAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCaa                                                                                                         >  6:288049/1‑148 (MQ=255)
                                                acacGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGc                                                                                                                                         <  6:212948/113‑1 (MQ=255)
                                                acacGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGc                                                                                                                                         >  5:212948/1‑113 (MQ=255)
                                                acacGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGAATCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACAtt                                                                                                     <  4:271285/149‑1 (MQ=255)
                                                acacGGTCCGGCAGAATATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCATACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACAtt                                                                                                     <  2:222211/149‑1 (MQ=255)
                                                 cacGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACAttt                                                                                                    <  1:236636/149‑1 (MQ=255)
                                                    ggTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAAt                                                                                                 <  4:169308/149‑1 (MQ=255)
                                                    ggTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAAt                                                                                                 <  2:25732/149‑1 (MQ=255)
                                                           cAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCttc                                                                                          <  2:306606/149‑2 (MQ=255)
                                                            tgATAATTTTACATGCTGTAGCCATGCGATAAGGTTTTCCGACGGTCGGTATGTTCCGTTGCAGACTCTTTAGGTTCAGATTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTc                                                                                         <  5:101279/148‑1 (MQ=255)
                                                             gATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCa                                                                                                                                 <  5:379746/108‑1 (MQ=255)
                                                             gATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCa                                                                                                                                 >  6:379746/1‑108 (MQ=255)
                                                              aTAATTTTACATGCTGTAGCCCAGCGATTAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCTTGTGACGTTGTAAGACCGCTTCAGTCCAAAATGTTCATTAATAGCAACATTTATAGCGCTATCg                                                                                        >  8:370550/1‑148 (MQ=255)
                                                              aTAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCg                                                                                        >  7:18915/1‑148 (MQ=255)
                                                               tAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGa                                                                                      <  7:280982/149‑1 (MQ=255)
                                                               tAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGa                                                                                      <  4:230669/149‑1 (MQ=255)
                                                                     tACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGt                                                                                                                               <  4:265266/102‑1 (MQ=255)
                                                                     tACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGt                                                                                                                               >  3:265266/1‑102 (MQ=255)
                                                                       cATGCTGTAGCCCAGCGATAAGGGTGGCCGACGGGCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGc                                                                               <  8:392633/148‑1 (MQ=255)
                                                                           cTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGGGTTCAGCGTtg                                                                           <  5:226207/148‑1 (MQ=255)
                                                                                 cccAGCGATAAGGTTGTCCTACGGTCGGTTTGTTCCGGTGCAGACTCTGGAGGGTCAGAGTTCCATGGGACGTTGTAAGCCAGCTTCAGTGCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTc                                                                    <  3:289786/149‑1 (MQ=255)
                                                                                 cccAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTc                                                                    <  1:30048/149‑1 (MQ=255)
                                                                                 cccAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTc                                                                    <  5:288049/149‑1 (MQ=255)
                                                                                     gCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTtgt                                                                          >  7:160387/1‑139 (MQ=255)
                                                                                     gCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTACATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTtgt                                                                          <  8:160387/139‑1 (MQ=255)
                                                                                     gCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGGTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCcgcg                                                                <  3:205426/149‑1 (MQ=255)
                                                                                       gATAAGGTTGTCCGACGGTCGGTATGTTCCGTTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCgcgc                                                               >  3:350631/1‑148 (MQ=255)
                                                                                         tAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCAATAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCa                                                             >  2:277982/1‑148 (MQ=255)
                                                                                            ggTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGa                                                         >  2:284790/1‑149 (MQ=255)
                                                                                                 tCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTc                                                                    >  1:131130/1‑133 (MQ=255)
                                                                                                 tCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTc                                                                    <  2:131130/133‑1 (MQ=255)
                                                                                                   cGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAAc                                                                                                        <  3:210035/95‑1 (MQ=255)
                                                                                                   cGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAAc                                                                                                        >  4:210035/1‑95 (MQ=255)
                                                                                                      cggtcggtATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACAcc                                               <  2:218159/149‑1 (MQ=255)
                                                                                                                 ttCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAAtt                                    <  8:187618/149‑1 (MQ=255)
                                                                                                                 ttCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAAtt                                    >  5:338117/1‑149 (MQ=255)
                                                                                                                  tCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAACCACCCTGCGTAAAtt                                    >  2:361852/1‑148 (MQ=255)
                                                                                                                     ggagCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGc                                <  3:343509/146‑1 (MQ=255)
                                                                                                                     ggTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGtt                                                                                   >  8:401762/1‑98 (MQ=255)
                                                                                                                     ggTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACAGAAACACCCTGCGTAAATTTAGc                                >  7:106746/1‑149 (MQ=255)
                                                                                                                        gCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTtgt                                                                          <  2:374815/104‑1 (MQ=255)
                                                                                                                                  gAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAAc                   >  4:42229/1‑149 (MQ=255)
                                                                                                                                         agagTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAACTGCCAt             <  6:338117/148‑1 (MQ=255)
                                                                                                                                          gagTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAACTGCCATGc           <  8:18915/149‑1 (MQ=255)
                                                                                                                                             ttCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAACTGCCATGCATa        <  8:414643/149‑1 (MQ=255)
                                                                                                                                                 aTGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAACTGCCATGCATAAGcc    >  6:252776/1‑149 (MQ=255)
                                                                                                                                                   gtgATGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAACTGCCATGCATAAGcccc  >  4:71311/1‑149 (MQ=255)
                                                                                                                                                   gtgACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAACTGCCATGCATAAGcccc  >  4:142874/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTCATTAATAGCAACATTTAATGCGCTTTCGGAGTTCAGCGTTGTGTCTTCCGCGCCAAAGACCGAAACACCCTGCGTAAATTTAGCGTTGTCAGTCAACTGCCATGCATAAGCCCC  >  NC_000913/1805257‑1805552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑