Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,806,335 G→T 100% intergenic (‑252/‑35) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,3350GT98.7% 261.1 / ‑3.7 76intergenic (‑252/‑35)ydiY/pfkBacid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (39/36);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTC  >  NC_000913/1806189‑1806474
                                                                                                                                                  |                                                                                                                                           
gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                           <  7:55953/149‑1 (MQ=255)
  gAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGc                                                                                                                                         >  6:226918/1‑149 (MQ=255)
   aaTAACTTAGCAATGTAATATTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTATTATTTAAATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGATAATTTCTTCACTTTCCGCTCATTCTGtcc                                                                                                                                         >  2:52617/1‑146 (MQ=255)
      aaCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGa                                                                                                                                     >  1:205525/1‑149 (MQ=255)
        cTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACt                                                                                                                                   >  2:87180/1‑149 (MQ=255)
        cTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACt                                                                                                                                   >  2:55869/1‑149 (MQ=255)
            gCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGaaa                                                                                                                               <  5:226918/149‑1 (MQ=255)
             cAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAAt                                                                                                                              >  3:273436/1‑149 (MQ=255)
              aaTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATc                                                                                                                             <  2:226382/149‑1 (MQ=255)
               aTGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCa                                                                                                                            <  5:177725/149‑1 (MQ=255)
                  tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGc                                                                                                                          <  8:399886/148‑1 (MQ=255)
                     tCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTg                                                                                                                                  >  4:335682/1‑137 (MQ=255)
                     tCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTg                                                                                                                                  <  3:335682/137‑1 (MQ=255)
                     tCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCta                                                                                                                       <  2:128362/148‑1 (MQ=255)
                          tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                           <  5:298349/123‑1 (MQ=255)
                          tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGt                                                                                                                                           >  6:298349/1‑123 (MQ=255)
                          tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATc                                                                                                                             >  3:151096/1‑137 (MQ=255)
                          tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATc                                                                                                                             <  4:151096/137‑1 (MQ=255)
                          tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATaggag                                                                                                                 >  6:318630/1‑149 (MQ=255)
                          tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATagga                                                                                                                  <  8:161584/148‑1 (MQ=255)
                              aTTTTTTGAATAAGCATGTTTCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGaaa                                                                                                             <  5:94756/149‑1 (MQ=255)
                              aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAGTCAGCGTATAGGAGGaaa                                                                                                             >  4:204508/1‑149 (MQ=255)
                                 ttttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAatga                                                                                                          >  7:369417/1‑149 (MQ=255)
                                    tGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATGTGCAAAATTTTAAATAAAGCTCCAATAAATCTTATTGTTAATTTCTTCACTTTCCGGTGATTCTGGGCCAGACTGAAATCAGGCTATAGGAGGAAatgatg                                                                                                        <  1:52617/148‑1 (MQ=255)
                                     gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                                       <  1:118817/148‑1 (MQ=255)
                                      aaTAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTa                                                                                                     >  5:293733/1‑149 (MQ=255)
                                        tAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACg                                                                                                   >  8:33121/1‑149 (MQ=255)
                                              tgtgGCCAAAACAGATTTTTAGTTATATATATATATCTGCAAAATTTAAAATAAAGCACAAATAAATAATATTGTTAAATTCTACACAATCAGCTCATTCTGTGCCAGACTGAAATCACCGTAGAGGAGGAAATGATGGTACGTATCta                                                                                             >  2:386164/1‑149 (MQ=255)
                                               gtgGCCAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGATACAATAAATCATATTGTTAATATGTTCACTTTCCGCTGATTCTGTGCCAGAATCAACTCAGCCTATAGGAGGAAATGATGGTACGTATCta                                                                                             >  4:92295/1‑148 (MQ=255)
                                                tgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtata                                                                                           <  7:62053/149‑1 (MQ=255)
                                                   cGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGt                                                                                        >  3:349673/1‑149 (MQ=255)
                                                     aaaaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                       >  2:69668/1‑148 (MQ=255)
                                                       aaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATaggag                                                                                                                 >  2:290164/1‑120 (MQ=255)
                                                       aaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATaggag                                                                                                                 <  1:290164/120‑1 (MQ=255)
                                                                ttatttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgcgc                                                                            <  2:205525/148‑1 (MQ=255)
                                                                   tttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                       <  8:68296/134‑1 (MQ=255)
                                                                   tttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                                       >  7:68296/1‑134 (MQ=255)
                                                                          atatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTg                                                                                      >  4:23811/1‑128 (MQ=255)
                                                                          atatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTg                                                                                      <  3:23811/128‑1 (MQ=255)
                                                                          atatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                                  >  2:381297/1‑148 (MQ=255)
                                                                           tatTTATCTGCAAAATTTTATATATAGCTCGAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                                <  8:369417/149‑1 (MQ=255)
                                                                           tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                                  >  4:142787/1‑147 (MQ=255)
                                                                           tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGAt                                                                 <  4:349673/148‑1 (MQ=255)
                                                                            atTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATc                                                                                                                             >  4:284548/1‑87 (MQ=255)
                                                                            atTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATc                                                                                                                             <  3:284548/87‑1 (MQ=255)
                                                                             tttATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAg                                                               <  6:293733/148‑1 (MQ=255)
                                                                                   tggaaaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                                                         >  4:127732/4‑148 (MQ=255)
                                                                                     cAAAATTTTAAATAAAGCTCCAATAACTCATATTCTTAATTGCTTCACTTTCCGCGGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaacaa                                                       <  6:130489/148‑1 (MQ=255)
                                                                                      aaaaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAAt                                                      >  5:379068/1‑148 (MQ=255)
                                                                                         aTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                                                  <  4:273436/149‑1 (MQ=255)
                                                                                          ttttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAccc                                                 >  1:104329/1‑149 (MQ=255)
                                                                                            ttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcccc                                                <  6:83685/148‑1 (MQ=255)
                                                                                                  aaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAtt                                         >  5:344947/1‑149 (MQ=255)
                                                                                                  aaaGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAtt                                         >  4:26877/1‑149 (MQ=255)
                                                                                                      cTCCAATAAATCATATTGTTTATTTCTTCACTTTCAGCTGATTCTGTGCCAGACTGTAATCAGCCTATAGGAGGAAATTATCGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATTGCGCAACAATTACCCCGCTAATTTAt                                      <  1:386164/148‑1 (MQ=255)
                                                                                                         cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCtat                                                                                                                      >  8:166769/1‑65 (MQ=255)
                                                                                                         cAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCtat                                                                                                                      <  7:166769/65‑1 (MQ=255)
                                                                                                         cAATAAATCATATTGTTAATTTCATCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAAGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATccc                                   >  8:175321/1‑148 (MQ=255)
                                                                                                           aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                                  <  2:148128/115‑1 (MQ=255)
                                                                                                           aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                                  >  1:148128/1‑115 (MQ=255)
                                                                                                           aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                                <  5:318630/149‑1 (MQ=255)
                                                                                                           aTAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                                <  7:326273/149‑1 (MQ=255)
                                                                                                             aaaTCATATTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAg                               >  6:46888/1‑148 (MQ=255)
                                                                                                                  atatTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGaaaa                          <  7:333395/148‑1 (MQ=255)
                                                                                                                  atatTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGaaaa                          <  1:87180/148‑1 (MQ=255)
                                                                                                                   tatTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACt                        >  4:16272/1‑149 (MQ=255)
                                                                                                                    atTGTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTg                       >  4:78747/1‑149 (MQ=255)
                                                                                                                       gTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg                     <  1:381297/148‑1 (MQ=255)
                                                                                                                       gTTAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcg                     <  1:55869/148‑1 (MQ=255)
                                                                                                                        ttAATTTCTTCACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCt                   >  3:84045/1‑149 (MQ=255)
                                                                                                                               cttcACTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTTTAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCg             >  8:343782/1‑148 (MQ=255)
                                                                                                                                    cTTTCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTGGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCgg       >  7:112758/1‑149 (MQ=255)
                                                                                                                                     tttCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGgt      >  6:17500/1‑149 (MQ=255)
                                                                                                                                       tCCGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGgt      <  3:26877/147‑1 (MQ=255)
                                                                                                                                         cGCTGATTCTGTGCCAGACTGAAATCAGCCTTTAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTc  <  7:343782/149‑1 (MQ=255)
                                                                                                                                         cGCTGATTCTGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTc  <  7:33121/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
GCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTC  >  NC_000913/1806189‑1806474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑