Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,965,904 A→G 5.4% I96I (ATT→ATC flhB ← flagellin export apparatus, substrate specificity protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,965,9040AG 5.4% 301.5 / 5.0 93I96I (ATT→ATCflhBflagellin export apparatus, substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base A (47/41);  new base G (2/3);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

GCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCC  >  NC_000913/1965766‑1966050
                                                                                                                                          |                                                                                                                                                  
gcCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCgg                                                                                                                                            >  3:24976/1‑147 (MQ=255)
gcCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGgca                                                                                                                                          <  6:273653/149‑1 (MQ=255)
 ccAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTGATTAGCGgcgt                                                                                                                                         <  6:41307/149‑3 (MQ=255)
 ccAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTGATTAGCGgcgt                                                                                                                                         <  6:35943/149‑3 (MQ=255)
      cTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAgcg                                                                                                                                    >  6:303144/1‑149 (MQ=255)
       tGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAgcgc                                                                                                                                   >  4:174827/1‑149 (MQ=255)
       tGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAgcgc                                                                                                                                   >  6:357971/1‑149 (MQ=255)
           ccGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGc                                                                                                                               <  1:197970/149‑1 (MQ=255)
            cGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCa                                                                                                                              <  3:69731/149‑1 (MQ=255)
             gagaACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCAt                                                                                                                             >  7:397535/1‑149 (MQ=255)
                aaCATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGc                                                                                                                          >  6:265704/1‑149 (MQ=255)
                  cATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCtt                                                                                                                        >  4:157288/1‑149 (MQ=255)
                    tCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTtct                                                                                                                      <  1:397830/149‑1 (MQ=255)
                    tCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTtct                                                                                                                      >  1:119295/1‑149 (MQ=255)
                     ccGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTGATTAGCGgcgtg                                                                                                                                        <  6:63207/130‑4 (MQ=255)
                     ccGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTGATTAGCGgcgtg                                                                                                                                        >  5:63207/1‑127 (MQ=255)
                     ccGTTAAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCAGCAAGGATTTGACGCTAAATACCAGCCCTCCAAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCAtctc                                                                                                                     >  3:298908/1‑149 (MQ=255)
                      cGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGAAGCAGCGCCAGCATGGCTTctct                                                                                                                    >  3:192965/1‑149 (MQ=255)
                            aTGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATcag                                                                                                              >  7:102231/1‑149 (MQ=255)
                            aTGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATca                                                                                                               <  8:397535/148‑1 (MQ=255)
                               cccGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATcag                                                                                                              >  8:124025/1‑146 (MQ=255)
                                 cGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGaa                                                                                                         <  3:51382/149‑1 (MQ=255)
                                    tAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCTGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGa                                                                                                          <  8:395511/145‑1 (MQ=255)
                                    tAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGa                                                                                                          >  3:158230/1‑145 (MQ=255)
                                    tAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGa                                                                                                          <  4:158230/145‑1 (MQ=255)
                                    tAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGa                                                                                                          >  7:395511/1‑145 (MQ=255)
                                      gCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGaataat                                                                                                     <  5:265704/148‑1 (MQ=255)
                                       cGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCt                                                                                                   >  2:77791/1‑149 (MQ=255)
                                        gggTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTg                                                                                                  <  2:200735/149‑1 (MQ=255)
                                            tGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGaataat                                                                                                     <  5:273763/142‑1 (MQ=255)
                                            tGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGaataat                                                                                                     >  6:273763/1‑142 (MQ=255)
                                               gTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAg                                                                                            >  4:25127/1‑148 (MQ=255)
                                                  tGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGAt                                                                                         <  1:77791/148‑1 (MQ=255)
                                                   ggAAAACTTCGGCTGCAAGTATTTTCCGCTAAATACCATCCCTCCCAGCATGACAGGAGAAATGAGAGCCACCAGCACCACTCCGGTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATc                                                                                        <  8:102231/148‑1 (MQ=255)
                                                   ggAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAACATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATc                                                                                        <  8:241932/148‑1 (MQ=255)
                                                    gAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAg                                                                                      <  8:36572/149‑1 (MQ=255)
                                                     aaaaCTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGa                                                                                     <  3:174827/149‑1 (MQ=255)
                                                       aaCTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGAtt                                                                                   <  5:201969/149‑1 (MQ=255)
                                                         cTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCg                                                                                 >  5:257325/1‑149 (MQ=255)
                                                          ttCGGCGGCAAGGATTTTCAGCTAAATACCAGCCCTCCCAGCATGACAGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGTATCAGATTCgg                                                                                <  4:298908/149‑1 (MQ=255)
                                                            cGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCgg                                                                                <  5:303144/147‑1 (MQ=255)
                                                                   aaGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGa                                                                        >  7:33000/1‑148 (MQ=255)
                                                                   aaGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGa                                                                        >  1:312617/1‑148 (MQ=255)
                                                                   aaGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCACGAGGATCAGATTCGGGTCATTGa                                                                        >  4:352323/1‑148 (MQ=255)
                                                                     gtaTTTGCCGCTAAATACCATCCCTCCCAGCATGACCGGAGAAATGAGCGCCACAAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCAGGGCTTCGCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGataa                                                                     <  6:161699/147‑1 (MQ=255)
                                                                      gATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGataa                                                                     >  3:371846/1‑148 (MQ=255)
                                                                           gCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGTGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACtgt                                                               >  4:240230/1‑149 (MQ=255)
                                                                           gCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGTGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACtgt                                                               >  4:235187/1‑149 (MQ=255)
                                                                             cGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGTGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGa                                                             <  3:235187/149‑1 (MQ=255)
                                                                             cGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGTGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGa                                                             <  3:240230/149‑1 (MQ=255)
                                                                             cGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACtgtg                                                              <  5:148649/148‑1 (MQ=255)
                                                                             cGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACtgtg                                                              >  6:148649/1‑148 (MQ=255)
                                                                               cTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGAt                                                            >  1:399251/1‑148 (MQ=255)
                                                                                  aaTACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCaaa                                                        <  2:399251/149‑1 (MQ=255)
                                                                                       cAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATg                                                     >  4:20767/1‑147 (MQ=255)
                                                                                       cAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCa                                                   <  3:25127/149‑1 (MQ=255)
                                                                                        aGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAg                                                  <  4:24976/149‑1 (MQ=255)
                                                                                        aGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAg                                                  <  2:312617/149‑1 (MQ=255)
                                                                                         gCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGc                                                 >  6:102102/1‑149 (MQ=255)
                                                                                          cccTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGcc                                                >  2:336392/1‑149 (MQ=255)
                                                                                           ccTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATc                                                           <  7:6115/137‑1 (MQ=255)
                                                                                           ccTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATc                                                           >  8:6115/1‑137 (MQ=255)
                                                                                            cTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGa                                                                                     <  3:382016/110‑1 (MQ=255)
                                                                                            cTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGa                                                                                     >  4:382016/1‑110 (MQ=255)
                                                                                             tCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAg                                             >  8:298049/1‑149 (MQ=255)
                                                                                             tCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAg                                             >  3:359725/1‑149 (MQ=255)
                                                                                               ccAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTATTCAGCGTCAGCAGCGACAGCATGGCTTCTCTTATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGct                                           >  3:114348/1‑148 (MQ=255)
                                                                                               ccAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCg                                           >  3:33419/1‑149 (MQ=255)
                                                                                                  gCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGataata                                                                   >  8:403422/1‑122 (MQ=255)
                                                                                                  gCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGataata                                                                   <  7:403422/122‑1 (MQ=255)
                                                                                                  gCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCg                                           >  2:312347/1‑146 (MQ=255)
                                                                                                   cATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTTGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGgaga                                       <  2:119295/149‑1 (MQ=255)
                                                                                                         cggtgtaaTGAGCGCGACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGTATGGCTGTTCTGATCAGCAGAATACTCTGCCCGTGGTGCAGATTCGGGTCATTGATAATACTGTTATCAAAATGCAGCCCAGCGGAGAGCATGc                                 <  1:90819/143‑1 (MQ=255)
                                                                                                         cGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCCCCAGCATGGCTTCTCTGATAAGCAGAATAATCTCCCCGAGGATCAGAATCGGGTCATTGATAATACTGTGATCAAAATGAAGCCCAGCGCAGAGc                                     >  3:294486/1‑145 (MQ=255)
                                                                                                           gagaAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGataata                                                                   >  6:337097/1‑113 (MQ=255)
                                                                                                           gagaAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGataata                                                                   <  5:337097/113‑1 (MQ=255)
                                                                                                                aTGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCa                                                          <  6:387150/117‑1 (MQ=255)
                                                                                                                aTGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCa                                                          >  5:387150/1‑117 (MQ=255)
                                                                                                                    gcgcCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGa                      >  4:28564/1‑149 (MQ=255)
                                                                                                                    gcgcCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGa                      <  8:33000/149‑1 (MQ=255)
                                                                                                                     cgcCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGAc                     <  5:357971/149‑1 (MQ=255)
                                                                                                                     cgcCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGAc                     <  3:28564/149‑1 (MQ=255)
                                                                                                                      gcCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCAtt                                                                          >  8:282470/1‑95 (MQ=255)
                                                                                                                      gcCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCAtt                                                                          <  7:282470/95‑1 (MQ=255)
                                                                                                                       ccaccaGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACgg                   <  2:168640/149‑1 (MQ=255)
                                                                                                                              caccacGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCg            <  3:20767/149‑1 (MQ=255)
                                                                                                                                ccacGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGa           >  1:213486/1‑148 (MQ=255)
                                                                                                                                  ctgccgcTGATTAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGaca         >  4:382036/3‑148 (MQ=255)
                                                                                                                                    gccgcTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGacacac      >  8:216944/1‑149 (MQ=255)
                                                                                                                                      cgcTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCg    <  3:157288/149‑1 (MQ=255)
                                                                                                                                       gcTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGc   >  5:13887/1‑149 (MQ=255)
                                                                                                                                         tAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACtgtg                                                              >  8:359336/1‑88 (MQ=255)
                                                                                                                                         tAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTAATCAAAATGCAGCCCCGCGGAGAGCAAGCCCGACAATCGACGGGCCAGCGACACACCgac  >  6:93827/1‑146 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
GCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGGGTTGAGTTTGGAAAACTTCGGCTGCAAGGATTTGCCGCTAAATACCAGCCCTCCCAGCATGACCGGAGAAATGAGCGCCACCAGCACCACGCCGCTAATCAGCGGCAGCAGCGCCAGCATGGCTTCTCTGATCAGCAGAATAATCTGCCCGAGGATCAGATTCGGGTCATTGATAATACTGTGATCAAAATGCAGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCC  >  NC_000913/1965766‑1966050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: