Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,980,240 C→A 5.5% S458I (AGC→ATC) ‡ otsA ← trehalose‑6‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,980,2400CA 5.5% 380.3 / 7.9 109S458I (AGC→ATC) ‡otsAtrehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (52/51);  new base A (4/2);  total (56/53)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01

Only 100 of 111 total aligned reads displayed.

AGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTGC  >  NC_000913/1980092‑1980385
                                                                                                                                                    |                                                                                                                                                 
aGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTACACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCgcgc                                                                                                                                                   >  6:246977/1‑149 (MQ=255)
 gTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCt                                                                                                                                                  >  2:234059/1‑149 (MQ=255)
  tttCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCtt                                                                                                                                                 >  1:281622/1‑149 (MQ=255)
     cTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcg                                                                                                                                               <  7:89084/148‑1 (MQ=255)
          cgcgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                                                                                          <  5:246977/148‑1 (MQ=255)
          cgcgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAAc                                                                                                                                         >  3:311227/1‑149 (MQ=255)
               gCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCt                                                                                                                                    >  5:318387/1‑149 (MQ=255)
                cATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCt                                                                                                                                    <  2:129151/148‑1 (MQ=255)
                  tCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGATATTAACAGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCt                                                                                                                                 >  1:100650/1‑149 (MQ=255)
                       cTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAg                                                                                                                             <  8:415394/148‑1 (MQ=255)
                          ggCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCg                                                                                                                         <  4:311227/149‑1 (MQ=255)
                           gCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCtt                                                                                                                                <  8:125001/141‑1 (MQ=255)
                           gCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCtt                                                                                                                                >  7:125001/1‑141 (MQ=255)
                           gCGAAAAGAGTTATCGCCTCACGGGAGGTCGATGTGCTGCTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGc                                                                                                                        <  2:281622/149‑1 (MQ=255)
                               aaaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAAt                                                                                                                    >  3:362538/1‑149 (MQ=255)
                               aaaGAGTTATTGCCTCAAGTGAGGACGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGATTTCCGCGCTTCGCGCAACTATCTGCTTTAGGTCGCTAAt                                                                                                                    >  6:119542/1‑149 (MQ=255)
                                 agagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTATTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGa                                                                                                                  <  2:190548/149‑1 (MQ=255)
                                  gagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGaa                                                                                                                 <  6:318387/149‑1 (MQ=255)
                                   agTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGATCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAg                                                                                                                <  6:187506/149‑1 (MQ=255)
                                        ttGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTc                                                                                                           >  7:339937/1‑149 (MQ=255)
                                        ttGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTc                                                                                                           <  5:169992/149‑1 (MQ=255)
                                           ccTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTg                                                                                                        >  6:380259/1‑149 (MQ=255)
                                            cTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAg                                                                                                                             <  7:124882/127‑1 (MQ=255)
                                             tCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGc                                                                                                       >  7:226401/1‑148 (MQ=255)
                                                aGTGAGGACGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGt                                                                                                   <  8:5820/149‑1 (MQ=255)
                                                 gTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTg                                                                                                  <  8:189413/149‑1 (MQ=255)
                                                 gTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCGCCTACGCACGCTTTGGAGAGGTAGCAACTTGATCGCGCTGCTGGCTTTCCGCGCGTCGCGGAACGATCTGCTTTAGGTCGCTAAGGAAGCAGGCGGGCCAGTg                                                                                                  >  4:55764/1‑149 (MQ=255)
                                                    aGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGtt                                                                                               <  1:28783/149‑1 (MQ=255)
                                                       tCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAat                                                                                            <  8:226401/149‑1 (MQ=255)
                                                             tgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGtt                                                                                      <  5:278861/149‑1 (MQ=255)
                                                              gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGc                                                                                                                                  <  3:160344/104‑1 (MQ=255)
                                                              gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGc                                                                                                                                  >  4:160344/1‑104 (MQ=255)
                                                              gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTc                                                                                                           >  5:106457/1‑127 (MQ=255)
                                                              gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGGAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTc                                                                                                           <  6:106457/127‑1 (MQ=255)
                                                              gCTGTTAGTTACACTTACGGGAGATTAACCGCTCCTGCGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGttt                                                                                     >  2:78466/1‑149 (MQ=255)
                                                                tGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTATTGAAGCAATCCTGCCAGTGGTTAATATCGttttt                                                                                   >  8:331183/1‑149 (MQ=255)
                                                                 gTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTATTTc                                                                                  >  2:308177/1‑149 (MQ=255)
                                                                  ttAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGttttt                                                                                   <  3:55764/147‑1 (MQ=255)
                                                                  ttAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTc                                                                                  <  5:21539/148‑1 (MQ=255)
                                                                  ttAGTGCCACGGACGGGAGATTAGGGGCTCCTACGCCAGCTTTGGAACGGTAGCAACTTTAGCGCGGTGCTGGCTTTCCGCGCTTCGGGGTTCTCTCAGCGTTAGGTCGCGATTGAAGCAGTCCTGCGAGGGGTTAATATCGGTTTTcc                                                                                 >  8:192970/1‑148 (MQ=255)
                                                                      ttCCACTTACGGGAGATTAACCACTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTATGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGa                                                                              >  5:56233/1‑148 (MQ=255)
                                                                       tCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCa                                                                                                              <  4:363543/115‑1 (MQ=255)
                                                                       tCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCa                                                                                                              >  3:363543/1‑115 (MQ=255)
                                                                         cACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCtt                                                                                                                                >  5:273086/1‑95 (MQ=255)
                                                                         cACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAAc                                                                          <  2:331315/149‑1 (MQ=255)
                                                                           cTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGt                                                                        <  1:8444/149‑1 (MQ=255)
                                                                            ttACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTc                                                                       >  3:130736/1‑149 (MQ=255)
                                                                                gggAGATTAACCGCTCCTCCGCAAGCTTTGGAAAGGTCGCAACTTTATCGCGCTGCTGGCTGTCCGCGCTTCGCGGAACTATCTGCGTTAGGTCGCTAATGAAGCACTCCTGCCAGGGGGTAATATCGTTTTTCACGATAACGTCCa                                                                     <  3:62595/147‑1 (MQ=255)
                                                                                gggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCACCTTTATCGCGCTGCTGGCTTTCCGCGCTGCGCGGAACTATCTGCTTTAGGTCGCTAATGCAGCACTCCTGCCAGGGGTTAATATCGTTTTTCACGATACCGTCCa                                                                     >  4:62595/1‑147 (MQ=255)
                                                                                gggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAg                                                                    >  7:48293/1‑148 (MQ=255)
                                                                                 ggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGcc                                                                                                      <  2:61851/113‑1 (MQ=255)
                                                                                 ggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGcc                                                                                                      >  1:61851/1‑113 (MQ=255)
                                                                                      ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCg                                                                                        >  2:303628/1‑122 (MQ=255)
                                                                                      ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCg                                                                                        <  1:303628/122‑1 (MQ=255)
                                                                                      ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCt                                                             <  5:143783/149‑1 (MQ=255)
                                                                                       tAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTg                                                            >  7:417/1‑149 (MQ=255)
                                                                                        aaCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTg                                                            <  5:380259/148‑1 (MQ=255)
                                                                                         aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCt                                                             >  6:215279/1‑146 (MQ=255)
                                                                                         aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCt                                                             <  5:215279/146‑1 (MQ=255)
                                                                                         aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCa                                                          <  6:246543/149‑1 (MQ=255)
                                                                                         aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCa                                                          <  3:34828/149‑1 (MQ=255)
                                                                                         aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCa                                                          <  4:130736/149‑1 (MQ=255)
                                                                                          ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCAt                                                         >  8:1248/1‑149 (MQ=255)
                                                                                          ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaag                                                                                                                                        <  8:277319/70‑3 (MQ=255)
                                                                                          ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaag                                                                                                                                        <  8:270881/70‑3 (MQ=255)
                                                                                          ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaag                                                                                                                                        >  7:270881/1‑68 (MQ=255)
                                                                                          ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaag                                                                                                                                        >  7:277319/1‑68 (MQ=255)
                                                                                            gCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGa                                                       <  8:48293/149‑1 (MQ=255)
                                                                                             cTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGAc                                                      >  8:269382/1‑149 (MQ=255)
                                                                                                atACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGAGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAATCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACggg                                                   <  7:331183/148‑1 (MQ=255)
                                                                                                    gCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGaaa                                                >  4:1553/1‑148 (MQ=255)
                                                                                                      aaGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATAc                                             >  8:331417/1‑149 (MQ=255)
                                                                                                      aaGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATAc                                             >  3:276648/1‑149 (MQ=255)
                                                                                                        gCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGt                                           <  3:1553/149‑1 (MQ=255)
                                                                                                         cTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGtt                                          >  7:292249/1‑149 (MQ=255)
                                                                                                            tGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAg                                                                    >  8:282685/1‑120 (MQ=255)
                                                                                                            tGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAg                                                                    <  7:282685/120‑1 (MQ=255)
                                                                                                            tGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTcc                                        <  8:417/148‑1 (MQ=255)
                                                                                                                      gCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACTGGAAATACTTTCCGCCAGCGACa                              >  2:122625/1‑148 (MQ=255)
                                                                                                                       cAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATa                            <  8:339937/149‑1 (MQ=255)
                                                                                                                       cAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATa                            <  1:311347/149‑1 (MQ=255)
                                                                                                                         aCTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGt                          <  7:269382/149‑1 (MQ=255)
                                                                                                                         aCTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCAGCCAGCGACa                              >  5:89130/1‑145 (MQ=255)
                                                                                                                         aCTTTATCGCGCTGATTTATTTCCGCGCTTCGCGGAACTATCTGCTTTAGTTCTCTTATGAAGAACTCCTGTCAGTGGTTAATATCTTTTTTCTCTATATGTTGCAGCATTTCTGCATGACGGGAATTACGTTCAGcctgataaa                              <  6:89130/145‑8 (MQ=255)
                                                                                                                             tATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCa                        <  1:234059/147‑1 (MQ=255)
                                                                                                                                cgcgCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGt                                           <  2:376663/125‑1 (MQ=255)
                                                                                                                                cgcgCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGt                                           >  1:376663/1‑125 (MQ=255)
                                                                                                                                cgcgCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCa                   >  7:77603/1‑149 (MQ=255)
                                                                                                                                     tgctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATc              >  7:297402/1‑149 (MQ=255)
                                                                                                                                       ctgGCTTTCCTCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATcc             <  2:100650/148‑1 (MQ=255)
                                                                                                                                       ctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCaa                       >  8:180585/1‑138 (MQ=255)
                                                                                                                                       ctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCaa                       <  7:180585/138‑1 (MQ=255)
                                                                                                                                         ggCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgc          >  7:157582/1‑149 (MQ=255)
                                                                                                                                         atagttCCGCGAAGCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgc          >  1:113700/5‑149 (MQ=255)
                                                                                                                                          gCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgcg         >  8:46273/1‑149 (MQ=255)
                                                                                                                                          gCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAAAACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgcg         >  7:407764/1‑149 (MQ=255)
                                                                                                                                             ttCCGCGAAGCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAg      >  8:325307/1‑149 (MQ=255)
                                                                                                                                                cgcgCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCt    >  1:400347/1‑148 (MQ=255)
                                                                                                                                                cgcgCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCt    <  2:400347/148‑1 (MQ=255)
                                                                                                                                                 gcgcTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTGc  <  7:46273/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTGC  >  NC_000913/1980092‑1980385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: