Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,525,312 T→A 5.0% G62G (GGT→GGA yfeN → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,3120TA 5.0% 245.4 / 3.4 80G62G (GGT→GGAyfeNputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (34/42);  new base A (2/2);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

ATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTACCTGGGTGACACCGGAT  >  NC_000913/2525165‑2525451
                                                                                                                                                   |                                                                                                                                           
atTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATATGAAGGTg                                                                                                                                            >  4:361365/1‑149 (MQ=255)
  tAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGgc                                                                                                                                          >  3:288062/1‑149 (MQ=255)
  tAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGgc                                                                                                                                          >  3:282928/1‑149 (MQ=255)
       aTACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTaa                                                                                                                                     >  7:392123/1‑149 (MQ=255)
              ttgtATCTCATGCAGAATTTAAATGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCAGGACTACCGAAAAATCTTCGACCCAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGt                                                                                                                              >  6:346915/1‑149 (MQ=255)
                gtATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTg                                                                                                                            >  7:112310/1‑149 (MQ=255)
                 tATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTgg                                                                                                                           <  7:3380/149‑1 (MQ=255)
                   actcATGCAGAATTTACAGGCGGTTTTGCAGATATCGGCGTCCACTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGCCCAAATCACCCAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTggg                                                                                                                          <  7:156276/147‑1 (MQ=255)
                         gCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAATATGATTTTGGTTATCTTGAATTTGAAGGAGgcgc                                                                                                                                        >  4:181782/1‑128 (MQ=255)
                         gCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGtt                                                                                                                             >  1:217972/1‑139 (MQ=255)
                         gCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGtt                                                                                                                             <  2:217972/139‑1 (MQ=255)
                         gCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGAGgcgc                                                                                                                                        >  4:187495/1‑128 (MQ=255)
                         gCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGAGgcgc                                                                                                                                        <  3:187495/128‑1 (MQ=255)
                         gCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGAGgcgc                                                                                                                                        <  3:181782/128‑1 (MQ=255)
                          cAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGCCTGGCCCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAAt                                                                                                                   <  6:263733/148‑1 (MQ=255)
                          cAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAAt                                                                                                                   <  8:112310/148‑1 (MQ=255)
                          cAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAAt                                                                                                                   <  7:228179/148‑1 (MQ=255)
                          cAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATg                                                                                                                  >  8:333652/1‑149 (MQ=255)
                           aGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTTGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTTGTTATCTTGAATTTTCAGGTGGCGCTAACTTTAGTTGGGGAGAAATGt                                                                                                                 <  6:367260/149‑1 (MQ=255)
                            gAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGtt                                                                                                                             >  2:347207/1‑136 (MQ=255)
                            gAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGtt                                                                                                                             <  1:347207/136‑1 (MQ=255)
                             aaTTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTAc                                                                                                               <  2:172746/149‑1 (MQ=255)
                             aaTTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTAc                                                                                                               <  5:323308/149‑1 (MQ=255)
                             aaTTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTAc                                                                                                               >  8:192278/1‑149 (MQ=255)
                               tttAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGga                                                                                                                        >  6:175774/1‑138 (MQ=255)
                                 tAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACggg                                                                                                            <  1:38668/148‑1 (MQ=255)
                                  aaaGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTAGCGAAATATCGGGGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGt                                                                                                           <  1:260164/148‑1 (MQ=255)
                                   aaGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGttc                                                                                                         >  5:192712/1‑149 (MQ=255)
                                    aGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGttct                                                                                                        >  2:303970/1‑149 (MQ=255)
                                      gCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGttcttc                                                                                                      >  2:52246/1‑149 (MQ=255)
                                        ggTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACgg                                                                                                             >  7:340597/1‑140 (MQ=255)
                                        ggTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACgg                                                                                                             <  8:340597/140‑1 (MQ=255)
                                        ggTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGa                                                                                                    <  4:288062/149‑1 (MQ=255)
                                        ggTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGa                                                                                                    <  4:282928/149‑1 (MQ=255)
                                          ttttGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACt                                                                                                  <  7:345652/149‑1 (MQ=255)
                                              gCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAAGGTACGGGTTCTGCGACTggg                                                                                               >  8:285089/1‑148 (MQ=255)
                                               cAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGaa                                                                                             <  8:392123/149‑1 (MQ=255)
                                                aGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGaa                                                                                             >  3:335259/1‑148 (MQ=255)
                                                  atatCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAAc                                                                                          >  1:11195/1‑149 (MQ=255)
                                                   tatCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACt                                                                                         >  1:413412/1‑149 (MQ=255)
                                                     tCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACtt                                                                                        <  6:192712/148‑1 (MQ=255)
                                                          gTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATaa                                                                                  <  7:278420/149‑1 (MQ=255)
                                                             cATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACgg                                                                               >  8:37070/1‑149 (MQ=255)
                                                             cATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACg                                                                                <  1:303970/148‑1 (MQ=255)
                                                                    tggactggacCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCa                                                                         >  3:74973/1‑148 (MQ=255)
                                                                      gactggacCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTggg                                                                                               >  7:244035/1‑124 (MQ=255)
                                                                      gactggacCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTggg                                                                                               <  8:244035/124‑1 (MQ=255)
                                                                      gactggacCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTAATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCa                                                                         <  7:108961/146‑1 (MQ=255)
                                                                           gacCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGAAGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTGTAGTTGGGCAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATacac                                                                 >  3:291046/1‑149 (MQ=255)
                                                                              cAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAg                                                              <  1:52246/149‑1 (MQ=255)
                                                                               aGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAgg                                                             <  2:413412/149‑1 (MQ=255)
                                                                                 ccGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCataat                                                                     <  3:165340/139‑1 (MQ=255)
                                                                                 ccGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCataat                                                                     >  4:165340/1‑139 (MQ=255)
                                                                                       tACCGAAAAATCGTCGACCAAATCACACAAATATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGag                                                      >  7:372787/1‑148 (MQ=255)
                                                                                          cGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCTACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGagcagc                                                  >  2:72388/1‑149 (MQ=255)
                                                                                          cGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCa                                                                         >  2:400368/1‑126 (MQ=255)
                                                                                          cGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCa                                                                         <  1:400368/126‑1 (MQ=255)
                                                                                           gAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAATACTTCTATAACGGGCGCAATAATAAACCAGGTAGCGagcaggg                                                 >  4:30492/1‑147 (MQ=255)
                                                                                            aaaaaTCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGt                                                <  6:359977/149‑1 (MQ=255)
                                                                                             aaaaTCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGt                                                >  1:46821/1‑148 (MQ=255)
                                                                                                    tcgACCAAATAACACAAAGATCAAATTGGTTATCTTGAATTTGAAGGTGGAGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAGAACTACTATAACGGTAGCCATAATAAACCAGGTAGCCAGCAGCGTAATACCtt                                        >  6:68525/1‑149 (MQ=255)
                                                                                                      gACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATAc                                           >  8:102741/1‑144 (MQ=255)
                                                                                                      gACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATAc                                           <  7:102741/144‑1 (MQ=255)
                                                                                                      gACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCttt                                       <  5:397362/148‑1 (MQ=255)
                                                                                                          aaaTCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTaaaaa                                  <  2:157382/149‑1 (MQ=255)
                                                                                                                 acaAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGTGTTATACCTTTaa                                     >  6:6619/1‑139 (MQ=255)
                                                                                                                 acaAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGTGTTATACCTTTaa                                     <  5:6619/139‑1 (MQ=255)
                                                                                                                   aaaGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGc                                                         >  3:139641/1‑117 (MQ=255)
                                                                                                                   aaaGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGc                                                         <  4:139641/117‑1 (MQ=255)
                                                                                                                     atatgatTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTa                       <  8:372787/147‑1 (MQ=255)
                                                                                                                     aGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTa                       <  7:333652/149‑1 (MQ=255)
                                                                                                                     aGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTa                       <  7:192278/149‑1 (MQ=255)
                                                                                                                           ttttGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTAcc                 <  2:46821/149‑1 (MQ=255)
                                                                                                                                gTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTACCTggg             <  3:361365/148‑1 (MQ=255)
                                                                                                                                gTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTACCTGGGt            <  4:74973/149‑1 (MQ=255)
                                                                                                                                      ttGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTACCTGGGTGACAcc      >  2:329981/1‑149 (MQ=255)
                                                                                                                                         aaTTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTACCTGGGTGACACCgg    <  4:335259/148‑1 (MQ=255)
                                                                                                                                          aTTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTACCTGGGTGACACCGGa   <  2:11195/148‑1 (MQ=255)
                                                                                                                                          aTTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTACCTGGGTGACACCGGAt  <  7:37070/149‑1 (MQ=255)
                                                                                                                                          aTTTGAAGGTGGCGCTAACTGTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAACCTTCTATAACGGTCTCCATACTAAACCATGTAGCGAGCAGCGTTATACCTTTAAAACTACCAACCGTATTTACCTGGGTGACACCGGa   <  7:285089/148‑1 (MQ=255)
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ATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTGCATTATCTGGACTGGACCAGCCGGACTACCGAAAAATCGTCGACCAAATCACACAAAGATGATTTTGGTTATCTTGAATTTGAAGGTGGCGCTAACTTTAGTTGGGGAGAAATGTACGGGTTCTTCGACTGGGAAAACTTCTATAACGGTCGCCATAATAAACCAGGTAGCGAGCAGCGTTATACCTTTAAAAATACCAACCGTATTTACCTGGGTGACACCGGAT  >  NC_000913/2525165‑2525451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: