Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,523,857 | G→T | 5.6% | S329S (TCG→TCT) | mrcA → | penicillin‑binding protein 1a, murein transglycosylase and transpeptidase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,523,857 | 0 | G | T | 5.6% | 239.2 / 3.5 | 72 | S329S (TCG→TCT) | mrcA | penicillin‑binding protein 1a, murein transglycosylase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base G (33/35); new base T (2/2); total (35/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01 |
CTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGC > NC_000913/3523710‑3524004 | cTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCgg > 6:58701/1‑149 (MQ=255) cTATGAAGACGGTTATCGCATTTACACCACCAACCCCCGCAAAGTGCATCAGGCCGCGCAGCAGGCGGTACGTAATAACGAGCAGGACTACGAAATCCGCCACGGCTATCTCGGCCCCGCAAATGAGCTCTAGAAAGTGGCCCAATCgg > 5:201723/1‑149 (MQ=255) aaGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTgg < 2:318167/149‑1 (MQ=255) aaGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCCAGTCGGCGTgg > 5:107949/1‑149 (MQ=255) tCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacaac > 1:392196/1‑148 (MQ=255) tCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacaac < 6:215464/148‑1 (MQ=255) gCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAg < 2:392196/149‑1 (MQ=255) cATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCTCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATatcaa < 3:88058/144‑1 (MQ=255) cATTTACACCACCATCACCCGCAAAGTGAAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGACAGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacaa > 4:88058/1‑144 (MQ=255) caccacCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCAGGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTAcc < 5:58701/148‑1 (MQ=255) caccacCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTAcc < 7:114296/148‑1 (MQ=255) accaccATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGa < 8:249030/149‑1 (MQ=255) caccaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTCCGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATa > 2:181825/1‑149 (MQ=255) caccaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCTCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTAc > 6:173723/1‑144 (MQ=255) caccaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTAc < 5:173723/144‑1 (MQ=255) caccaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGa < 7:62959/147‑1 (MQ=255) caccaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGa > 8:62959/1‑147 (MQ=255) caTCACCCGCAAAGTGCAGCCGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACCACCAGATTACCGATACGc > 7:351974/1‑149 (MQ=255) tCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCt > 7:190327/1‑148 (MQ=255) aCCCGCACACTGCCGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGaa < 8:190327/149‑1 (MQ=255) aCCAGCAAAGTGCATGAGGCCGCGCAGTAGGCGGTACGTAATAACGTGATTGACTTCTAAATGCGCCAGGGCTATCGCGGCCCGGCAATTGTGTTGTGGAATGTGGGCGTGTCGGCGTGGGATTACTTGATGATTACCGATACGCTGa < 6:107949/148‑1 (MQ=255) cccGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAg < 6:189070/149‑1 (MQ=255) cccGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAg > 5:189070/1‑149 (MQ=255) gCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCCCGGCTATCGCCGCCCGGCAAATGGGCGGTGGAAAGTGGGCGAGGCGGGGTGGGTGAAGAACAAGATTACGGGTAGGCTGCAGgcg > 2:392250/1‑149 (MQ=255) cAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTTGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTTGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGgcgc < 3:279896/149‑1 (MQ=255) aaaGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCt < 8:386054/149‑1 (MQ=255) aGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGc < 8:190501/149‑1 (MQ=255) tagcagGCCTCGCAGCAGGCTGTTCGTACTAACGTGCTGGACTACGACATTCGCCACGGCTATCGCGGCACTGCAAATGGGCTGTGGAAAGTTGGCGAGGCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAAc < 8:380899/148‑1 (MQ=255) cagcagGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAAc < 8:135752/149‑1 (MQ=255) agcagGCCGCGCAGCATGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACACGATTACCGATACGCTGa < 5:116430/135‑1 (MQ=255) agcagGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacaac < 1:251193/116‑1 (MQ=255) agcagGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacaac > 2:251193/1‑116 (MQ=255) agcagGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGa > 6:116430/1‑135 (MQ=255) cgcgCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGCGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTc > 5:374211/1‑149 (MQ=255) cGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGTAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGctgcct < 5:94553/149‑1 (MQ=255) ataaCGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGACGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGt < 1:181825/148‑1 (MQ=255) gTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAg > 3:16098/1‑149 (MQ=255) gCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAgcg > 5:81592/1‑149 (MQ=255) gCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAgcg > 2:270164/1‑149 (MQ=255) gACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCaa > 7:400571/1‑149 (MQ=255) gACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCa > 4:164001/1‑148 (MQ=255) gACTACGACATGCCCCACGGCTATCGAGGCCATGAAAAAGTGAAGAGGAAAGTTGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTCCCAACCTATCGTCCGCTGCTTCCTGCCGCAGACACAAgctaaa > 6:49653/1‑144 (MQ=255) tACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCTGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCg < 7:329072/117‑1 (MQ=255) tACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCTGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCg < 7:323374/117‑1 (MQ=255) tACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCTGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCg > 8:329072/1‑117 (MQ=255) tACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCTGCGAGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCg > 8:323374/1‑117 (MQ=255) cGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGAt < 4:198911/67‑1 (MQ=255) cGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGAt > 3:198911/1‑67 (MQ=255) gACATGCGCCCCGGCTATCGCGGCCCGGCAAATGGGCTGGGGAACGGGGGCGAGTCGGCGGGGGAAAACAACAAGATAACCGATACGCGGAAGGCGCTGCCAACCTATGGTCCGCTGCGGCCTGCCGCAGTCACCAGCGCCAAGCCTCa < 6:374211/149‑1 (MQ=255) aTGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGc > 8:55765/1‑148 (MQ=255) aTGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGACTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGc > 2:363673/1‑148 (MQ=255) gcgcCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAg > 1:183513/1‑149 (MQ=255) gcCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCa < 6:167536/95‑1 (MQ=255) gcCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCa > 5:167536/1‑95 (MQ=255) ccACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCCCCAGCGCCAATCCTCAGCAAGCGa > 8:318087/1‑149 (MQ=255) cGGCTATCGCGGCCCGGCAAATGTGCTGGGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACgg < 3:298735/149‑1 (MQ=255) aTCGCGGCCCGGCAAAGGTGCTGTGGAAAGTGGGCGAGGCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCGGCCGCCCTATGGTCCGCTGCTGCCTGCCGCAGGCACCAGCGCCAATCCTCCGCAAGCGCCGGCGagg > 6:100140/1‑147 (MQ=255) gcgGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTg < 1:270164/149‑1 (MQ=255) gcgGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTg > 7:128610/1‑149 (MQ=255) gcgGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTg > 5:40908/1‑149 (MQ=255) gCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCg > 4:125000/1‑149 (MQ=255) cGGCAAATGTGCTGTGGAAAGTGGGCGAGGCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGAc < 4:380204/149‑1 (MQ=255) gCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACgg < 6:40908/149‑1 (MQ=255) gCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGACTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACgg < 1:363673/149‑1 (MQ=255) aaaTGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGAc < 5:294654/145‑1 (MQ=255) aaaTGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGAc > 6:294654/1‑145 (MQ=255) aaaTGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGt < 3:164001/149‑1 (MQ=255) aaaTGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGt > 2:310483/1‑149 (MQ=255) tgGAAAGTGCGCGAGTCGGCGTGGGATAACAACACGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTc < 2:218027/147‑1 (MQ=255) gTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAg < 1:310483/149‑1 (MQ=255) ggCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTAt > 7:132372/1‑149 (MQ=255) gCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATg > 4:215963/1‑149 (MQ=255) gAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGa < 3:215963/149‑1 (MQ=255) cGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGc < 8:400571/149‑1 (MQ=255) | CTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGC > NC_000913/3523710‑3524004 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |