Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913169,0150GA65.7% 112.7 / 98.5 105W511* (TGG→TAG) fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (17/19);  new base A (36/33);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 105 total aligned reads displayed.

AAACAAACGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAA  >  NC_000913/168870‑169160
                                                                                                                                                 |                                                                                                                                                 
aaacaaacGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCg                                                                                                                                                >  3:291254/1‑149 (MQ=255)
aaacaaacGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGCGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCg                                                                                                                                                >  2:126108/1‑149 (MQ=255)
aaacaaacGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCATAGTCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCg                                                                                                                                                >  1:270477/1‑149 (MQ=255)
 aacaaacGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCgt                                                                                                                                               >  5:153691/1‑149 (MQ=255)
  acaaccGGGCGTTTATGTTCAGGATCAGGGGCAGTGGGATAAAGTGCTGGTCACGCTAGGCGGGCGTTATGACTGGGAAGAGCAAGAATCTCTTAACCGCGTTGGCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCgtt                                                                                                                                              <  7:347017/149‑2 (MQ=255)
  acaaacGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCgtg                                                                                                                                              >  1:494724/1‑149 (MQ=255)
    aaacGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCgtggt                                                                                                                                            >  6:421304/1‑149 (MQ=255)
     aacGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCgtggtg                                                                                                                                           >  1:34108/1‑149 (MQ=255)
     aacGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCgtggtg                                                                                                                                           <  3:346875/149‑1 (MQ=255)
        ggtcGTTTATGTTCAGGATCAGGAGCAGTTGTATAAAGTGCTGGGCACCCTAGGCGGTCGTTATGAATGGGCAGATCAAGACTCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCgtggtggtg                                                                                                                                        <  3:6433/146‑1 (MQ=255)
          gCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGtt                                                                                                                                      <  7:403268/149‑1 (MQ=255)
          gCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGGTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGtt                                                                                                                                      <  5:95423/149‑1 (MQ=255)
            gTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTaa                                                                                                                                    <  5:452105/149‑1 (MQ=255)
            gTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTaa                                                                                                                                    <  4:291254/149‑1 (MQ=255)
             tttATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAAc                                                                                                                                   >  8:367416/1‑149 (MQ=255)
                   ttCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTg                                                                                                                             >  6:302428/1‑149 (MQ=255)
                      aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCCCCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGttt                                                                                                                          >  1:103156/1‑149 (MQ=255)
                      aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGtt                                                                                                                           <  5:302428/148‑1 (MQ=255)
                      aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGttt                                                                                                                          <  8:337099/149‑1 (MQ=255)
                      aGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGtt                                                                                                                           <  5:69594/148‑1 (MQ=255)
                             ggCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATg                                                                                                                   <  7:45787/149‑1 (MQ=255)
                             ggCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATg                                                                                                                   <  4:216718/149‑1 (MQ=255)
                               cgcAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGgt                                                                                                                 <  6:153691/149‑1 (MQ=255)
                                gcAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGgtg                                                                                                                >  7:217025/1‑149 (MQ=255)
                                      ggATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAacac                                                                                                          >  4:306826/1‑149 (MQ=255)
                                       gagacaGTGCGGGTCACCCTAGGCGGTCGTTATGACTCGGCAGATCAAGAATCTCTTCACCGCGGTGCCGGGAGGACCGATAAAGGTGATGACAAACAGTTTACCTGGCGTCGTGGTGTTAACTACCTGTTTCATAATTGTGTAACTcc                                                                                                         <  6:332195/144‑1 (MQ=255)
                                        aTAAAGTGCTGGTCACGCTTGGCGGTCGTTATGACTGGGCAGTTCATGAATCTCGTAACCTCGTGTCCGGGACGACCGATAACCGTGATGACAAACAGTTTACCGGGCGTGGTGGTGTTAACTAACTGGGTGATAATGGTGTAACACCt                                                                                                        <  1:126108/149‑1 (MQ=255)
                                        aTAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGCCCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCt                                                                                                        >  7:11720/1‑149 (MQ=255)
                                         tAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCtt                                                                                                       <  7:367416/149‑1 (MQ=255)
                                                cTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAg                                                                                                >  6:263659/1‑149 (MQ=255)
                                                cTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAg                                                                                                <  2:202647/149‑1 (MQ=255)
                                                  ggTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGc                                                                                               >  3:108895/1‑148 (MQ=255)
                                                   gTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGc                                                                                               <  2:270477/147‑1 (MQ=255)
                                                     cACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCgtggtggt                                                                                                                                         <  5:475799/103‑1 (MQ=255)
                                                     cACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCgtggtggt                                                                                                                                         >  6:475799/1‑103 (MQ=255)
                                                       cccTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGAGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGc                                                                                         >  3:413915/1‑149 (MQ=255)
                                                        ccTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGc                                                                                         >  3:111829/1‑148 (MQ=255)
                                                            ggCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTCACTTCAGCTATAGCGAATc                                                                                    <  3:306826/149‑1 (MQ=255)
                                                              cGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGt                                                                                  <  5:263659/149‑1 (MQ=255)
                                                              cGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGt                                                                                  <  4:305618/149‑1 (MQ=255)
                                                                gTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGttt                                                                                <  4:111829/149‑1 (MQ=255)
                                                                   gTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGa                                                                              <  6:58020/148‑1 (MQ=255)
                                                                     tATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTc                                                                                                  >  8:413426/1‑126 (MQ=255)
                                                                     tATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTc                                                                                                  <  7:413426/126‑1 (MQ=255)
                                                                     tATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAcc                                                                           >  8:158808/1‑149 (MQ=255)
                                                                         aCTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGgtgt                                                                                                               <  3:235144/109‑1 (MQ=255)
                                                                         aCTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGgtgt                                                                                                               >  4:235144/1‑109 (MQ=255)
                                                                             ggCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCttcttc                                                                     <  5:421304/147‑1 (MQ=255)
                                                                               cAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCaa                                                                 <  4:231652/149‑1 (MQ=255)
                                                                                aGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAg                                                                >  8:175031/1‑149 (MQ=255)
                                                                                  aTCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACAcc                                                                                                         >  8:299520/1‑106 (MQ=255)
                                                                                   tCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTg                                                             >  7:324288/1‑149 (MQ=255)
                                                                                    cAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGttt                                                                                <  4:93994/129‑1 (MQ=255)
                                                                                    cAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGttt                                                                                >  3:93994/1‑129 (MQ=255)
                                                                                              ttAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGa                                                      <  7:158808/145‑1 (MQ=255)
                                                                                                aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTaa                                                >  7:446665/1‑149 (MQ=255)
                                                                                                aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTaa                                                >  6:98307/1‑149 (MQ=255)
                                                                                                aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGAGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                   <  4:413915/146‑1 (MQ=255)
                                                                                                aaCCGCCTTGCAGGTAAGACCTATAACCGTGATGACAAACAGTTTACATGGCGTGTTGGTGTTAACTAACTGTTTGATAATGGTGTCACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGTGAAGGATgt                                                   <  8:217025/146‑2 (MQ=255)
                                                                                                 aCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTTGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAat                                               <  2:494724/149‑1 (MQ=255)
                                                                                                 aCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTaa                                                                                                                                    <  5:421380/64‑1 (MQ=255)
                                                                                                 aCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTaa                                                                                                                                    >  6:421380/1‑64 (MQ=255)
                                                                                                  ccGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAata                                              >  4:38019/1‑149 (MQ=255)
                                                                                                   cgcgTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGaa                                                         >  6:460537/1‑137 (MQ=255)
                                                                                                   cgcgTTGCC‑GGACGACCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATAtt                                            >  6:337688/1‑149 (MQ=255)
                                                                                                               acgacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCt                                                                                                                              >  4:5132/1‑56 (MQ=255)
                                                                                                               acgacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCt                                                                                                                              <  3:5132/56‑1 (MQ=255)
                                                                                                                 gacCGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCt                                >  8:400030/1‑148 (MQ=255)
                                                                                                                   ccGATAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTa                               >  6:156078/1‑147 (MQ=255)
                                                                                                                    cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAg                            >  1:405322/1‑149 (MQ=255)
                                                                                                                    cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAg                            <  7:175031/149‑1 (MQ=255)
                                                                                                                      aTAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAc                                                                            <  4:182810/99‑1 (MQ=255)
                                                                                                                      aTAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAAc                                                                            >  3:182810/1‑99 (MQ=255)
                                                                                                                      aTAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                   <  1:42385/124‑1 (MQ=255)
                                                                                                                      aTAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                   >  2:42385/1‑124 (MQ=255)
                                                                                                                      aTAAACGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGt                          <  4:108895/149‑1 (MQ=255)
                                                                                                                         aaCGTGATGACAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAg                       >  5:353285/1‑149 (MQ=255)
                                                                                                                           cGTGATGACAAACAGTTTTCCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTGCTTCGCAAGTTGGGAAGGATGTTAATATTTTCGCACCGTCTAAAGGTAATCa                     <  8:11720/149‑1 (MQ=255)
                                                                                                                             tgatgaCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCg                                                                    >  1:14358/1‑100 (MQ=255)
                                                                                                                             tgatgaCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCg                                                                    <  2:14358/100‑1 (MQ=255)
                                                                                                                                tgaCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTAt                 >  3:379478/1‑148 (MQ=255)
                                                                                                                                  aCAAACAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATg                >  8:430347/1‑147 (MQ=255)
                                                                                                                                  aCAAACAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGaa              <  2:34108/149‑1 (MQ=255)
                                                                                                                                    aaaCAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGt            >  4:300028/1‑149 (MQ=255)
                                                                                                                                    aaaCAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGt            >  2:142728/1‑149 (MQ=255)
                                                                                                                                    aaaCAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGt            >  3:380985/1‑149 (MQ=255)
                                                                                                                                     aaCAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTc           <  7:351150/149‑1 (MQ=255)
                                                                                                                                     aaCAGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTc           >  8:351150/1‑149 (MQ=255)
                                                                                                                                     aaCAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTc           >  6:397452/1‑149 (MQ=255)
                                                                                                                                      aCAGTTTACCTAGCGTGGAGCTCTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCCATAGAACCTACTTCGCAAGTTGGGAAGGATGGAAATATTTTCGCACCGTCTAAACGAAAGCAGTATGAAGtag          >  2:353225/1‑147 (MQ=255)
                                                                                                                                       cAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAg                                                                >  2:255621/1‑94 (MQ=255)
                                                                                                                                       cAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAg                                                                <  1:255621/94‑1 (MQ=255)
                                                                                                                                       cAGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCgg         >  3:179239/1‑149 (MQ=255)
                                                                                                                                        aGTTTACCTGGCGTGGTGGTGTTAACTAACTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGc        <  3:300028/149‑1 (MQ=255)
                                                                                                                                        aGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAg                                                        >  1:66538/1‑101 (MQ=255)
                                                                                                                                        aGTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAg                                                        <  2:66538/101‑1 (MQ=255)
                                                                                                                                         gTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCGTACTTCAGCTATAGCGATTCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGGAATATATTCGCACCGTCTAATGGTAAGCAGTATGATGTCggag       >  1:421536/1‑147 (MQ=255)
                                                                                                                                         gTTTACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACATTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCg       >  1:414663/1‑149 (MQ=255)
                                                                                                                                          tttACCTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGt      >  7:424221/1‑149 (MQ=255)
                                                                                                                                              ccTAGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGaaa  <  5:156078/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AAACAAACGGGCGTTTATGTTCAGGATCAGGCGCAGTGGGATAAAGTGCTGGTCACCCTAGGCGGTCGTTATGACTGGGCAGATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAA  >  NC_000913/168870‑169160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑