New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 895626 =26 (0.710)6 (0.170) 6/252 3.5 19.8% coding (636/1134 nt) potG putrescine ABC transporter ATPase
?NC_000913 895654 = 24 (0.690)coding (664/1134 nt) potG putrescine ABC transporter ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTT  >  NC_000913/895510‑895800
                                                                                                                                                |                                                                                                                                                  
gATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTAt                                                                                                                                                <  2:181784/149‑1 (MQ=255)
                     gATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCa                                                                                                                           <  1:152507/149‑1 (MQ=255)
                         aaaaGCTGCGTGACAGGATGCAGCTTGAAGGGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGAtt                                                                                                                       >  1:113648/1‑149 (MQ=255)
                              cTGCGTGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGa                                                                                                                  <  2:113648/149‑1 (MQ=255)
                                     aCAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGCTGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCAGCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGaa                                                                                                           <  4:34726/149‑1 (MQ=255)
                                        ggATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAagag                                                                                                        <  7:86485/149‑1 (MQ=255)
                                                  tGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGaa                                                                                                           <  4:53617/136‑1 (MQ=255)
                                                  tGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGaa                                                                                                           >  3:53617/1‑136 (MQ=255)
                                                         gtggATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGAc                                                                                       >  6:135719/1‑149 (MQ=255)
                                                            gATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTg                                                                                                                      >  4:40379/1‑115 (MQ=255)
                                                            gATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTg                                                                                                                      <  3:40379/115‑1 (MQ=255)
                                                            gATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTAc                                                                                    >  4:119560/1‑149 (MQ=255)
                                                                ttCTGGCGCGCGGCGGTGTGACTTGTGTGCTGGTCACCCACGAGCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGc                                                                                <  7:49058/149‑1 (MQ=255)
                                                                     gAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAg                                                                           >  5:166677/1‑149 (MQ=255)
                                                                            tCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGaa                                                                    <  3:119560/149‑1 (MQ=255)
                                                                               gtgtGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAAtt                                                                  >  8:7583/1‑148 (MQ=255)
                                                                                           tgATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGt                                                      <  5:135719/148‑1 (MQ=255)
                                                                                               ggTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTAt                                                               >  2:51522/1‑135 (MQ=255)
                                                                                               ggTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTAt                                                               <  1:51522/135‑1 (MQ=255)
                                                                                                   aCCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCtt                                             >  1:44385/1‑149 (MQ=255)
                                                                                                             aGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCg                                                        >  2:17274/1‑128 (MQ=255)
                                                                                                             aGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCg                                                        <  1:17274/128‑1 (MQ=255)
                                                                                                                          gACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGt                       >  6:162712/1‑148 (MQ=255)
                                                                                                                                       cgcATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTANACTNCTTTNAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCt         <  5:162712/149‑1 (MQ=255)
                                                                                                                                          aTCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGt      <  2:159865/149‑1 (MQ=255)
                                                                                                                                          aTCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGt      <  7:7583/149‑1 (MQ=255)
                                                                                                                                             gCCATTATGAAGAGTGGGAAATTTGTCCAGATTGGAGAACCGGAAGAGATCTACGAGCATGCGACTACCCGCTATAGCGCCGATTTTATTGGCTCGGTATACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCt   <  2:44385/149‑1 (MQ=255)
                                                                                                                                               cATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCtt  >  6:73767/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTCCAGATTGGCGAACCGGAAGAGATCTACGAGCATCCGACTACCCGCTATAGCGCCGAATTTATTGGCTCGGTAAACGTCTTTGAAGGCGTACTCAAAGAGCGTCAGGAAGATGGCCTGGTGCTT  >  NC_000913/895510‑895800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.