New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4194511 =85 (1.050)3 (0.040) 3/252 NT 3.9% coding (1589/1896 nt) thiC phosphomethylpyrimidine synthase
?NC_000913 4194541 = 65 (0.830)coding (1559/1896 nt) thiC phosphomethylpyrimidine synthase
Rejected: Frequency below/above cutoff threshold.

ATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGACCTGGCGAAAGGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4194662‑4194511
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcaGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCG  >  NC_000913/4194541‑4194688
                                                                                                                                                                                                                                                                                                            
ATTGGTGCGGCTATGATTGGCTGGTGTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGACCTGGCGAAAGG                                                                                                                                                         <  5:344698/149‑1
ATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGACCTGGCGAAAGG                                                                                                                                                         <  2:196521/149‑1
         GCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGACCTGGCGAAAGGGCAGCcggg                                                                                                                                                >  6:320163/1‑145
                                        TCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGACCTGGCGAAAGG                                                                                                                                                         <  3:91395/109‑1
                                        TCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGACCTGGCGAAAGG                                                                                                                                                         >  4:91395/1‑109
                                                                                                              CTATAAGATTGCTGCCCACGCCGCTGACCTGGCGAAAGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCCTCGCGCAGCCAAACCAGCCAAT                                           >  1:304220/1‑149
                                                                                                                           GCCCACGCCGCTGACCTGGCGAAAGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCG                                                                                <  3:73138/99‑1
                                                                                                                           GCCCACGCCGCTGACCTGGCGAAAGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCG                                                                                >  4:73138/1‑99
                                                                                                                                                    GGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGC     <  8:58763/149‑1
                                                                                                                                                       AGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCG  >  5:287137/1‑149
                                                                                                                                                                                                                                                                                                            
ATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGACCTGGCGAAAGGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4194662‑4194511
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcaGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCG  >  NC_000913/4194541‑4194688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.