| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 603,688 | T→A | 5.8% | T326S (ACC→TCC) | ybdG ← | mechanosensitive channel protein, miniconductance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 603,688 | 0 | T | A | 5.8% | 240.8 / 3.5 | 79 | T326S (ACC→TCC) | ybdG | mechanosensitive channel protein, miniconductance |
| Reads supporting (aligned to +/- strand): ref base T (37/35); new base A (3/2); total (40/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAA > NC_000913/603540‑603834 | tatTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGACAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGCTAGGCACGAAAGGt > 8:319536/1‑149 (MQ=255) ttCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTc > 5:71259/1‑149 (MQ=255) ttCCAGACACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTc < 3:258280/149‑1 (MQ=255) ccAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTcc < 1:253113/148‑1 (MQ=255) cAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCaa > 8:314744/1‑149 (MQ=255) cAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCaa < 7:266658/149‑1 (MQ=255) cAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCaa > 5:333573/1‑149 (MQ=255) aGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCaa > 7:31562/1‑148 (MQ=255) cacaCCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGt < 1:242387/140‑1 (MQ=255) cacaCCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGt > 2:242387/1‑140 (MQ=255) acacCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGGTCCAATATTg < 1:242916/149‑1 (MQ=255) accacGGTGTTAGTAAACGCGTAGATCTCGAGCGTTAAACAATAATAACATGAAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCTGATATTCGTTCAGATCGGCACGAAAGGTTCCAATATTGGt > 8:80895/1‑149 (MQ=255) accacGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGt > 3:219405/1‑149 (MQ=255) ggTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTc < 6:286121/149‑1 (MQ=255) ttAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGgcg < 2:266448/149‑1 (MQ=255) aGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGgcg > 7:182943/1‑147 (MQ=255) gTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAg < 6:333573/149‑1 (MQ=255) gTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCAATAAGGTCATATCTTTACGAATCCCCGGATGGTTACGCACATATTCGTACAGATAGGAACGAAAGGTTACAATATTGGTCATTCGgcg > 1:332291/1‑146 (MQ=255) gcgTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGTTTACGCAGATATTCGTTCAGATAGGCACGAAAGGATCCAATATTGGTCATTCGGCGCAGATTTaa > 3:276690/1‑149 (MQ=255) gcgTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTaa < 8:134509/149‑1 (MQ=255) gcgTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGATCCAATATTGGTCATTCGGCGCAGATTTaa > 3:281333/1‑149 (MQ=255) gcgTAGAGCTCGAGCTGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCACAGATTTaa < 4:182674/149‑1 (MQ=255) gATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATAcc > 7:23604/1‑148 (MQ=255) aTCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCg < 8:31562/148‑1 (MQ=255) tcGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTc > 7:367776/1‑149 (MQ=255) gAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGa < 2:193330/144‑1 (MQ=255) aGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCTGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGt < 3:181585/149‑1 (MQ=255) aGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGt < 7:314744/149‑1 (MQ=255) gTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGaa > 4:316276/1‑149 (MQ=255) gTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGGAGaa > 7:373487/1‑149 (MQ=255) tAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGAc > 6:291052/1‑139 (MQ=255) tAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGAc < 5:291052/139‑1 (MQ=255) tAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATAATCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAAc > 6:41050/1‑149 (MQ=255) aTCAGCTGGAGCCAGTTGGCGTAACATTAAGGTCATATCTTTAGTAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCAGGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTgg < 2:332291/148‑1 (MQ=255) aTCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTgg < 7:212382/148‑1 (MQ=255) aCCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGa < 3:234964/148‑1 (MQ=255) aCCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGa < 8:319286/148‑1 (MQ=255) cTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTc > 4:209234/1‑149 (MQ=255) gAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCAc < 6:71259/149‑1 (MQ=255) gCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGt > 5:194310/1‑125 (MQ=255) gCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGt < 6:194310/125‑1 (MQ=255) cGTCCCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTc < 8:356542/149‑1 (MQ=255) gTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTcc > 5:192004/1‑149 (MQ=255) tACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCt > 1:70139/1‑149 (MQ=255) ccATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGa > 7:120747/1‑149 (MQ=255) ttAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTACAGATAGGCACGAAAGGTTCCAAAATTGGTCATTCGGCGCAGATTTAATACCGACGCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTAGCTGATgg > 5:78182/1‑149 (MQ=255) tCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCTGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTg > 4:300646/1‑149 (MQ=255) tttACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCCCTCATTAATTTCCTGATGGCGGCTGGTTAAAt > 4:275849/1‑149 (MQ=255) attACGAATCCGCGGATGGTTACAAAGATATTCGTTCAGATAGGCACGAAAGGATCCAATATTGGTCATTCGGCCCAGAATTAATACCGACTCATTAGAACCTTGCTGGCGATTACACTCATTAATTTACAGATGGCGGATGGTTaaa > 6:159516/2‑148 (MQ=255) tCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCttt < 8:23604/149‑1 (MQ=255) tCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCtt > 4:189291/1‑148 (MQ=255) cgcgGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTaa < 4:219405/149‑1 (MQ=255) cgcgGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTaa < 8:367776/149‑1 (MQ=255) ttACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATgcgc < 3:189291/149‑1 (MQ=255) ttACGCAGATATGCGTTCAGATAGGCACGAAAGGGTCCAATATTGGTCTTTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGAGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATgcgc < 3:229772/149‑1 (MQ=255) cGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCtt > 3:335879/1‑148 (MQ=255) gCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTa > 3:131286/1‑149 (MQ=255) aGATATTCGTTCAGATAGGCACGAAAGGATCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTAtt < 4:281333/149‑1 (MQ=255) aGATATTCGTTCAGATAGGCACGAAAGGATCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCAACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTAtt < 4:276690/149‑1 (MQ=255) gATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTc < 5:159516/149‑1 (MQ=255) ttCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGa < 2:70139/148‑1 (MQ=255) ttCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCtt < 5:306664/122‑1 (MQ=255) ttCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCtt > 6:306664/1‑122 (MQ=255) ataTAGTCACGAAATGTTCCAATATTTGTCATTAGGCGAATATTTAATACCGACTCCTTAGAACCTTGATGGCTATTCCACTCATTAATTTCCTGATGTCGGCTGGTTAAATAAGGCTTTAACAAATTCGCTTTATTCAGACGTTGCa < 1:171319/146‑1 (MQ=255) aGATAGGTACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCAt > 8:261601/1‑149 (MQ=255) aGATAGGCACGAAAGTTTCCAATATTTGTCATTCGGCGCAGATTTAATACCGACTCCGTAGATCCTTGCTGGCGATTCCACTCATTAATTTCCTGTTGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCa < 3:316276/148‑1 (MQ=255) tAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTc > 3:125196/1‑149 (MQ=255) ggCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGt > 4:241409/1‑149 (MQ=255) gCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTAtt > 4:294746/1‑131 (MQ=255) gCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTAtt < 3:294746/131‑1 (MQ=255) gCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTc > 5:63878/1‑149 (MQ=255) cacctaaGGTTCCTTTATTGGTCAGTCGGAGCATATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGGGGCTGGGTAAATCAGGCGTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCt < 6:78182/144‑1 (MQ=255) aCGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCtt > 6:158462/1‑149 (MQ=255) aCGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCtt > 6:139643/1‑149 (MQ=255) cGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCtt > 1:28006/1‑148 (MQ=255) cGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTtc < 7:319536/149‑1 (MQ=255) cGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTtc < 6:192004/149‑1 (MQ=255) gAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAAt > 6:49828/1‑102 (MQ=255) gAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTtca > 7:22856/1‑149 (MQ=255) ggTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCaa < 8:182943/149‑1 (MQ=255) ggTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCaa < 7:261601/149‑1 (MQ=255) gTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTtcatc > 7:272774/1‑146 (MQ=255) | TATTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCATATCTTTACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAA > NC_000913/603540‑603834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |