Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,351,482 G→A 5.5% A515V (GCG→GTG)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,351,4820GA 5.5% 233.0 / 2.2 73A515V (GCG→GTG) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base G (41/28);  new base A (2/2);  total (43/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

CATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCC  >  NC_000913/3351334‑3351624
                                                                                                                                                    |                                                                                                                                              
cATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACg                                                                                                                                                >  8:344002/1‑149 (MQ=255)
  tctcCAGCGCCGCCTTGCCGGTCATGGCGATATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGc                                                                                                                                               >  2:287258/1‑148 (MQ=255)
  tctcCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGACCGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGTCATATCGTCTTCATCAAACGca                                                                                                                                              >  2:111095/1‑149 (MQ=255)
         cgccgcCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCatcatcta                                                                                                                                       <  5:29136/149‑3 (MQ=255)
          gccgccTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGAc                                                                                                                                      <  5:307068/149‑1 (MQ=255)
               cTTGCCGGTCATGGCGTCATCTAGGCTGTTACCTAATTTTTCCAGCACAGAACGCTCAAAAATGACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATTTCGTCTTGATCAAACGCATCATCGACCtctt                                                                                                                                 <  5:218098/149‑1 (MQ=255)
                ttGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCtcttc                                                                                                                                >  1:129091/1‑149 (MQ=255)
                    cGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCGTCGACCTCTTCTGc                                                                                                                             >  7:352714/1‑148 (MQ=255)
                         aTGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATTTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATATAACGCATCATCGACCTCTTCAGCTACCGa                                                                                                                       >  5:181146/1‑149 (MQ=255)
                         aTGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGa                                                                                                                       <  3:287227/149‑1 (MQ=255)
                            gCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACgg                                                                                                                    <  2:305407/149‑1 (MQ=255)
                                 aTCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACTATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGt                                                                                                               >  2:120656/1‑149 (MQ=255)
                                 aTCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGt                                                                                                               >  8:122812/1‑149 (MQ=255)
                                  tCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGATCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTg                                                                                                              >  3:197452/1‑149 (MQ=255)
                                     aCGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTg                                                                                                                  >  1:363501/1‑142 (MQ=255)
                                     aCGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTg                                                                                                                  <  2:363501/142‑1 (MQ=255)
                                      cGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATc                                                                                                          <  1:287258/149‑1 (MQ=255)
                                      cGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATc                                                                                                          <  6:132156/149‑1 (MQ=255)
                                              ccTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACg                                                                                                   >  8:9101/1‑148 (MQ=255)
                                               cTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGt                                                                                                  >  4:59025/1‑148 (MQ=255)
                                               cTAATATTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCAACCAGCAGCACATTCAGCGCCGGAAAAGGCATATCGTCGTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGt                                                                                                  >  5:206980/1‑148 (MQ=255)
                                                 aaTTTTTACAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTa                                                                                                 >  2:225246/1‑147 (MQ=255)
                                                          aGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACACATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAAcc                                                                                      >  3:100324/1‑149 (MQ=255)
                                                          aGCACAGAACGCGCAACAATCACGGTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAAcc                                                                                      >  4:317875/1‑149 (MQ=255)
                                                             acaGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCttt                                                                                   <  7:122812/149‑1 (MQ=255)
                                                             acaGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCttt                                                                                   >  4:283521/1‑149 (MQ=255)
                                                             acaGAACGCGCAACAATCACGTTCAGTTCAATGGCTTCCACCAGCCGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCttt                                                                                   <  2:162638/149‑1 (MQ=255)
                                                               aGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGc                                                                                 >  8:194632/1‑149 (MQ=255)
                                                                   cgcgCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGCCCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTg                                                                             >  2:45008/1‑149 (MQ=255)
                                                                     cgcAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGtt                                                                           <  1:120656/149‑1 (MQ=255)
                                                                      gcAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTc                                                                          <  7:194632/149‑1 (MQ=255)
                                                                        aacaaTCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGTGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGACATGTTCGc                                                                        >  6:43980/1‑149 (MQ=255)
                                                                         acaaTCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACACATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCt                                                                       <  4:100324/149‑1 (MQ=255)
                                                                          caaTCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGAGCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTg                                                                      <  4:165137/149‑1 (MQ=255)
                                                                           aaTCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACACATCATCGAcc                                                                                                                                     <  8:70478/85‑1 (MQ=255)
                                                                           aaTCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACACATCATCGAcc                                                                                                                                     >  7:70478/1‑85 (MQ=255)
                                                                             tCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGt                                                                    >  4:193518/1‑148 (MQ=255)
                                                                             tCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGt                                                                    >  1:370495/1‑148 (MQ=255)
                                                                                  ttcagttcaATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCg                                                               >  7:322422/1‑148 (MQ=255)
                                                                                    cagttcaATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTa                                                             <  2:370495/148‑1 (MQ=255)
                                                                                     agttcaATGTCTTCCACCAGCATCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTaa                                                            <  4:197452/148‑1 (MQ=255)
                                                                                      gttcaATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAata                                                          <  3:59025/149‑1 (MQ=255)
                                                                                       ttcaATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAatat                                                         >  1:230387/1‑149 (MQ=255)
                                                                                         caATTTCTTCCACCAGCAGCACATTCAGTGACGGTAAAGGCATATCGTCTTCATCATACGCATCATCGAACTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAAAATTTGCCCTGTTCGCTGGTAACCGTAATATc                                                        <  5:43980/148‑1 (MQ=255)
                                                                                          aaTGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATcgc                                                      >  8:240301/1‑149 (MQ=255)
                                                                                               cTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTaaa                                                                                               <  8:210332/103‑1 (MQ=255)
                                                                                               cTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTaaa                                                                                               >  7:210332/1‑103 (MQ=255)
                                                                                                ttCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATAACGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCa                                                >  8:245761/1‑149 (MQ=255)
                                                                                                        gcagcaCATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTc                                        <  3:283521/149‑1 (MQ=255)
                                                                                                         cagcaCATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCg                                       >  2:134837/1‑149 (MQ=255)
                                                                                                            cacaTTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCa                                    <  1:225246/149‑1 (MQ=255)
                                                                                                            cacaTTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCa                                    <  4:341829/149‑1 (MQ=255)
                                                                                                               aTTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTc                                        >  2:248602/1‑142 (MQ=255)
                                                                                                               aTTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTc                                        <  1:248602/142‑1 (MQ=255)
                                                                                                                    gcgcCGGTATAGGCTTATCGTCTTCATCACACGCCTCATCGACCTCTTCTGCTACGTACGGTGCGTGGATCGTGAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGAcgacga                            <  1:111095/149‑1 (MQ=255)
                                                                                                                    gcgcCGGTAAAGGCATATCGTCTTCATCAAACGCATCGTCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGAcgacga                            <  8:352714/149‑1 (MQ=255)
                                                                                                                    gcgcCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGAcgacga                            <  7:344002/149‑1 (MQ=255)
                                                                                                                             aaGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGcc                   <  7:9101/149‑1 (MQ=255)
                                                                                                                                gCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGa                >  8:158393/1‑149 (MQ=255)
                                                                                                                                gCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGAGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGa                >  8:162016/1‑149 (MQ=255)
                                                                                                                                 cATATCGGCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGAAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGAc               <  2:129091/149‑1 (MQ=255)
                                                                                                                                  atatCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGACCCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGAc               >  5:156353/1‑148 (MQ=255)
                                                                                                                                  atatCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGAc               >  4:138936/1‑148 (MQ=255)
                                                                                                                                        tCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGATGAGAAACGGCCAGACCAATAcc        >  7:332765/1‑149 (MQ=255)
                                                                                                                                        tCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATAcc        >  3:160840/1‑149 (MQ=255)
                                                                                                                                          ttCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTTACCGTAATATCGACGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGCCCAATAccgg      >  6:313879/1‑149 (MQ=255)
                                                                                                                                          ttCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACgaga                          <  7:77829/129‑1 (MQ=255)
                                                                                                                                          ttCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACgaga                          >  8:77829/1‑129 (MQ=255)
                                                                                                                                          ttCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATAccgg      >  6:5919/1‑149 (MQ=255)
                                                                                                                                          ttCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATAccgg      >  4:254505/1‑149 (MQ=255)
                                                                                                                                          ttCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATAccgg      >  3:191029/1‑149 (MQ=255)
                                                                                                                                             atcaAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATAccggtgc   >  3:16984/1‑149 (MQ=255)
                                                                                                                                              tcaAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATAccggtgcc  >  1:126754/1‑149 (MQ=255)
                                                                                                                                                 aaCGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCTCCAGacgacgc                            >  6:19600/1‑119 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAGGCATATCGTCTTCATCAAACGCATCATCGACCTCTTCTGCTACCGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCC  >  NC_000913/3351334‑3351624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: