Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,448,278 C→A 5.1% intergenic (‑129/+36) rplN ← / ← rpsQ 50S ribosomal subunit protein L14/30S ribosomal subunit protein S17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,2780CA 5.1% 402.6 / 2.5 120intergenic (‑129/+36)rplN/rpsQ50S ribosomal subunit protein L14/30S ribosomal subunit protein S17
Reads supporting (aligned to +/- strand):  ref base C (50/63);  new base A (4/3);  total (54/66)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTCTCG  >  NC_000913/3448131‑3448425
                                                                                                                                                   |                                                                                                                                                   
tAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAACAAATAAACGGCTCATTTCt                                                                                                                                                    >  4:49260/1‑149 (MQ=255)
tAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCt                                                                                                                                                    >  5:29543/1‑149 (MQ=255)
  gTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCt                                                                                                                                                    >  5:290899/1‑147 (MQ=255)
  gTCTGTTCTTAGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGa                                                                                                                                                  <  3:77723/149‑1 (MQ=255)
   tCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAg                                                                                                                                                 >  8:238471/1‑149 (MQ=255)
   tCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAg                                                                                                                                                 >  8:20303/1‑149 (MQ=255)
       ttCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGcc                                                                                                                                               <  7:124748/147‑1 (MQ=255)
            ggATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGTCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGttt                                                                                                                                           <  5:124978/146‑1 (MQ=255)
            ggATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTAtt                                                                                                                                        >  6:218310/1‑149 (MQ=255)
            ggATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGTTTAt                                                                                                                                         <  4:31197/148‑1 (MQ=255)
             gATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTc                                                                                                                                       >  6:62096/1‑149 (MQ=255)
              aTCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCg                                                                                                                                      >  7:105463/1‑149 (MQ=255)
              aTCATTTTAGTGCTCCCCTAATGTCAACTACAACAGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGAAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCg                                                                                                                                      >  2:359981/1‑149 (MQ=255)
                  ttttAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTAtt                                                                                                                                  <  8:188538/149‑1 (MQ=255)
                  ttttAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTAtt                                                                                                                                  <  1:150922/149‑1 (MQ=255)
                     tAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTga                                                                                                                                <  6:312301/148‑1 (MQ=255)
                     tAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTga                                                                                                                                <  7:238471/148‑1 (MQ=255)
                     tAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTga                                                                                                                                <  8:133345/148‑1 (MQ=255)
                     tAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTga                                                                                                                                <  2:9927/148‑1 (MQ=255)
                        tGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTgagaga                                                                                                                            <  3:314313/149‑1 (MQ=255)
                          cTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgt                                                                                                                          <  7:20303/149‑1 (MQ=255)
                           tCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgt                                                                                                                          >  8:331309/1‑148 (MQ=255)
                           tCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGTTTa                                                                                                                                          >  8:179558/1‑132 (MQ=255)
                           tCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGTTTa                                                                                                                                          <  7:179558/132‑1 (MQ=255)
                             cGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTgaga                                                                                                                              <  4:243105/142‑1 (MQ=255)
                             cGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTa                                                                                                                       >  6:179281/1‑149 (MQ=255)
                             cGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTATGAGCCGTTTATTCGTATTgaga                                                                                                                              >  3:243105/1‑142 (MQ=255)
                               cTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTGAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACt                                                                                                                     <  3:264999/149‑1 (MQ=255)
                                  atgtatactagtactGAGACCCTAAATTCAGGGCGTTAATAATGCCCATATCGAGGGCGCGTCATTATAACACCGCTTCTAGGATATGGTTAGAAAAATTAATCGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTa                                                                                                                  <  1:116169/143‑1 (MQ=255)
                                   tGTCAACTACTACTGAGACCCGAAAATCTGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCGGCAAGGAGATGGGTAGAAAAAATAAACGGCTAATTTCTGAGACGTGTATTCGTATTGAGAGAGTGTAcgttat                                                                                                                 >  5:323662/1‑144 (MQ=255)
                                   tGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTAt                                                                                                                 >  2:369812/1‑149 (MQ=255)
                                   tGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTAt                                                                                                                 <  6:290899/149‑1 (MQ=255)
                                   tGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTAt                                                                                                                 >  3:330088/1‑149 (MQ=255)
                                   tGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTAt                                                                                                                 >  2:352203/1‑149 (MQ=255)
                                       aaCTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTa                                                                                                               >  4:77095/1‑147 (MQ=255)
                                       aaCTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTGTATTCGGATTGAGAGAGTGTACTGTATTa                                                                                                               >  7:35305/1‑147 (MQ=255)
                                            ctactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGaa                                                                                                          <  8:291819/147‑1 (MQ=255)
                                            ctactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGaa                                                                                                          <  5:218310/147‑1 (MQ=255)
                                             tactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCg                                                                                                       >  7:274868/1‑149 (MQ=255)
                                               ctGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGGTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCt                                                                                                     >  6:180132/1‑149 (MQ=255)
                                                tGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGc                                                                                                                                                <  2:156419/105‑1 (MQ=255)
                                                tGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgtg                                                                                                                         >  3:120180/1‑128 (MQ=255)
                                                tGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgtg                                                                                                                         <  4:120180/128‑1 (MQ=255)
                                                  agaCCCGAAAATCAGGTCTTTAAAAATCCCCATATCGAGGGCGCGGCTTTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCttt                                                                                                   <  7:229260/148‑1 (MQ=255)
                                                  agaCCCGAAAATCAGCTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTtc                                                                                                  <  2:256638/149‑1 (MQ=255)
                                                       cGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCttt                                                                                                   >  3:196883/1‑143 (MQ=255)
                                                       cGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCttt                                                                                                   <  4:196883/143‑1 (MQ=255)
                                                        gAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTAc                                                                                             >  8:197947/1‑148 (MQ=255)
                                                           aaTCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAAcg                                                                                         <  1:139064/149‑1 (MQ=255)
                                                               aGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTAc                                                                                             <  6:106746/141‑1 (MQ=255)
                                                               aGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTAc                                                                                             >  5:106746/1‑141 (MQ=255)
                                                               aGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGaa                                                                                     >  4:332923/1‑149 (MQ=255)
                                                                ggTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTa                                                                                                                                          <  6:196534/95‑1 (MQ=255)
                                                                ggTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAAc                                                                                    >  8:195391/1‑149 (MQ=255)
                                                                 gTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAAcc                                                                                   <  8:105463/149‑1 (MQ=255)
                                                                       aaaaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGt                                                                                                                                             <  5:108444/85‑1 (MQ=255)
                                                                       aaaaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGt                                                                                                                                             >  6:108444/1‑85 (MQ=255)
                                                                        aaaaTCTCCATATCGAGGGCGCGGGATTATTACTAGTCTGCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGATCCGTTTATTCGTATTGAGAGAGGGTACTGTATTACAGAAGCGCTTTCTCTACAACGCGAACCAGCGTc                                                                             <  5:247083/148‑1 (MQ=255)
                                                                         aaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTc                                                                             >  4:76130/1‑147 (MQ=255)
                                                                         aaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCa                                                                           <  1:4205/149‑1 (MQ=255)
                                                                            tCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTAc                                                                                                              >  1:346768/1‑111 (MQ=255)
                                                                            tCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTAc                                                                                                              <  2:346768/111‑1 (MQ=255)
                                                                            tCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGa                                                                        >  3:151907/1‑149 (MQ=255)
                                                                            tCCACATATCGCCGGCGCGGCATTATAACACCGCTTCAAGGATATGTGTAGAAAAAATAAACGGCGCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACACCGCGAACCAGCGTCCAGGa                                                                        <  2:271701/149‑1 (MQ=255)
                                                                                cATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTa                                                                    <  3:297349/149‑1 (MQ=255)
                                                                                   atCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTc                                                                 >  5:360234/1‑149 (MQ=255)
                                                                                    tCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTc                                                                 <  5:179281/148‑1 (MQ=255)
                                                                                      gAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTg                                                              <  7:195391/149‑1 (MQ=255)
                                                                                        gtgcgcGGCATTCTAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGa                                                            <  5:186180/147‑1 (MQ=255)
                                                                                          gcgcGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGACCCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACa                                                          >  1:281983/1‑149 (MQ=255)
                                                                                          gcgcGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGa                                                            >  1:277011/1‑147 (MQ=255)
                                                                                           cgcgGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAg                                                         <  7:89318/149‑1 (MQ=255)
                                                                                                 aTTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACg                                                   <  3:73303/149‑1 (MQ=255)
                                                                                                     taACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCAt                                               <  1:333952/149‑1 (MQ=255)
                                                                                                     taACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCAt                                               >  1:58003/1‑149 (MQ=255)
                                                                                                     taACACCGCTGCCAGGATATGTGTAGAAAAAATAACCGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGAAGGCAt                                               <  6:323662/149‑1 (MQ=255)
                                                                                                           cGCTTCAAGTATATGGGTAGAAAAAATTAACTGATCATTTCTGAGTCGTTTATTCGTATTTAGAGAGTTTACTGTATTACAGTACCGCTTTCTCTTCAACGCGAACCAGCGTCCATGATTTAGTCTTGGACAGCGGACGGCTTTcgctg                                         <  2:219911/149‑3 (MQ=255)
                                                                                                            gCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGa                                        <  6:263550/149‑1 (MQ=255)
                                                                                                             cTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGAt                                       <  3:76130/149‑1 (MQ=255)
                                                                                                               tCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGAttt                                     >  5:32108/1‑149 (MQ=255)
                                                                                                                cAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGAtt                                      <  2:309434/147‑1 (MQ=255)
                                                                                                                 aaGGATGTGGGTAGAAAAAATAAACGGCTGATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTc                                    >  3:184831/1‑148 (MQ=255)
                                                                                                                    gATATGGGGAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTCTTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAAcc                                <  6:215193/149‑1 (MQ=255)
                                                                                                                      tatGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCAc                              <  4:330088/149‑1 (MQ=255)
                                                                                                                          ggTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCa                          >  5:255265/1‑149 (MQ=255)
                                                                                                                               aaataaTTAACTGCTCATTTCTGAGCCGTTTATTAGTATTGAGAGAGTGTACTGTATTACATAACCGCTTTCTCTACAACGCGAACCAGCGTCGAGTATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGAt                     <  1:359981/149‑1 (MQ=255)
                                                                                                                               aaaaaaTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCAc                              <  1:209136/140‑1 (MQ=255)
                                                                                                                               aaaaaaTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCAc                              >  2:209136/1‑140 (MQ=255)
                                                                                                                               aaaaaaTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGa                      >  5:91502/1‑148 (MQ=255)
                                                                                                                                 aaaaTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATAc                   >  4:257658/1‑149 (MQ=255)
                                                                                                                                     tAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCa               >  7:318157/1‑149 (MQ=255)
                                                                                                                                       aaCGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCAtt             <  8:274868/149‑1 (MQ=255)
                                                                                                                                       aaCGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCAtt             >  2:124498/1‑149 (MQ=255)
                                                                                                                                         cGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCg           <  6:360234/149‑1 (MQ=255)
                                                                                                                                           gCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgtt         <  8:124784/149‑1 (MQ=255)
                                                                                                                                             tCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACTGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgttgt       >  1:210542/1‑149 (MQ=255)
                                                                                                                                             tCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgtt         <  5:353015/147‑1 (MQ=255)
                                                                                                                                             tCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgtt         >  6:353015/1‑147 (MQ=255)
                                                                                                                                                  tCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTtctc   <  1:60960/148‑1 (MQ=255)
                                                                                                                                                   cTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTCTCg  <  3:77095/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTCTCG  >  NC_000913/3448131‑3448425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: