Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,576,161 G→C 5.6% Q301E (CAA→GAA)  gntU ← gluconate transporter, low affinity GNT 1 system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,576,1610GC 5.6% 231.5 / 3.1 68Q301E (CAA→GAA) gntUgluconate transporter, low affinity GNT 1 system
Reads supporting (aligned to +/- strand):  ref base G (36/28);  new base C (2/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

AGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTT  >  NC_000913/3576015‑3576307
                                                                                                                                                  |                                                                                                                                                  
aGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTg                                                                                                                                                    >  5:260857/1‑147 (MQ=255)
  cTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAgc                                                                                                                                                >  3:226687/1‑149 (MQ=255)
      cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGt                                                                                                                                            >  7:172638/1‑149 (MQ=255)
      cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGt                                                                                                                                            <  2:324433/149‑1 (MQ=255)
      cAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGt                                                                                                                                            <  2:103429/149‑1 (MQ=255)
       aGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGt                                                                                                                                            >  1:147916/1‑148 (MQ=255)
               gCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCaaa                                                                                                                                   <  2:70232/149‑1 (MQ=255)
                 aGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAAtc                                                                                                                                 >  7:209298/1‑149 (MQ=255)
                  ggTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGCCCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAAtct                                                                                                                                >  4:66261/1‑149 (MQ=255)
                   gTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGCACACGCCGCCCGCCCCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGCCCGCAAAtctc                                                                                                                               >  1:178351/1‑149 (MQ=255)
                    tGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTcc                                                                                                                              <  1:200933/149‑1 (MQ=255)
                       tAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCCCCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATc                                                                                                                           >  7:265172/1‑149 (MQ=255)
                        aGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCCCCAATCACCAGCAGAATGATCCACGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCa                                                                                                                          >  2:200080/1‑149 (MQ=255)
                         gCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGGGCCCGCAAATCTCCATCAc                                                                                                                         >  1:363972/1‑149 (MQ=255)
                                    gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTg                                                                                                              <  8:172638/149‑1 (MQ=255)
                                    gCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTg                                                                                                              <  2:147916/149‑1 (MQ=255)
                                      ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAgcgc                                                                                                                                              >  7:375827/1‑115 (MQ=255)
                                      ccATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAgcgc                                                                                                                                              <  8:375827/115‑1 (MQ=255)
                                              gTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCCCCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGCCCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTg                                                                                                    >  2:235261/1‑149 (MQ=255)
                                                   cGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCa                                                                                               >  1:286433/1‑149 (MQ=255)
                                                   cGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCa                                                                                               >  3:303134/1‑149 (MQ=255)
                                                     cTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCAtt                                                                                             <  7:12542/149‑1 (MQ=255)
                                                         gCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGAAGATTGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCa                                                                                         <  6:93371/149‑1 (MQ=255)
                                                           ccAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGa                                                                                       <  4:226687/149‑1 (MQ=255)
                                                               tGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGa                                                                                                                                          >  8:328318/1‑94 (MQ=255)
                                                               tGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGa                                                                                                                                          <  7:328318/94‑1 (MQ=255)
                                                                        tACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGcca                                                                          >  6:190226/1‑149 (MQ=255)
                                                                          cGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGccac                                                                         >  5:153085/1‑148 (MQ=255)
                                                                             cAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCCCCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGa                                                                     >  1:128451/1‑149 (MQ=255)
                                                                             cAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGa                                                                     <  2:286433/149‑1 (MQ=255)
                                                                             cAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGa                                                                     >  7:308104/1‑149 (MQ=255)
                                                                                agTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGAc                                                                    >  3:164830/1‑147 (MQ=255)
                                                                                  tCAACCAGCACCTGTTTGAAAACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAg                                                                 >  3:75569/1‑148 (MQ=255)
                                                                                    aaCCAGCACCTGTTTGAAAACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCaa                                                              <  4:75569/149‑1 (MQ=255)
                                                                                     aCCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAAc                                                             <  5:190226/149‑1 (MQ=255)
                                                                                     aCCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGACATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAAc                                                             <  3:36112/149‑1 (MQ=255)
                                                                                      ccAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAAcc                                                            <  2:3222/149‑1 (MQ=255)
                                                                                         gCACCTGTTTGAACACGCCGCCCGCCCCAATCACCATCAGAATGATCCCCGCCGGTTGCAGCGCGAGACCGCAAATATCCATCACTTTGTCTTATGTAATGCCCTGACGCAATGCCAGACCCGAAATCGCCACCAGCAAAGCAACCAg                                                          >  4:99248/1‑148 (MQ=255)
                                                                                            ccTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATc                                                      >  2:67726/1‑149 (MQ=255)
                                                                                             cTGTTTGAACACGCCGCCTGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCCCTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATcg                                                     >  4:68080/1‑149 (MQ=255)
                                                                                               gTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATcgcg                                                   >  8:208887/1‑149 (MQ=255)
                                                                                               gTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGAACGTAAATCGACACCAGACAAGCACCCAGAATcgcg                                                   >  4:75695/1‑149 (MQ=255)
                                                                                                tttGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATcgcg                                                   <  6:153085/148‑1 (MQ=255)
                                                                                                  tGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGCCGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCggg                                                 >  7:149516/1‑147 (MQ=255)
                                                                                                   gAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTa                                                <  4:164830/148‑1 (MQ=255)
                                                                                                         gccgccCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGAt                                         >  8:94575/1‑149 (MQ=255)
                                                                                                              ccgcACCAATCACCAGCAGAATGATCCCCGCCGGTTCCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGccc                                                                                                      >  5:64176/1‑83 (MQ=255)
                                                                                                              ccgcACCAATCACCAGCAGAATGATCCCCGCCGGTTCCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGccc                                                                                                      <  6:64180/83‑1 (MQ=255)
                                                                                                              ccgcACCAATCACCAGCAGAATGATCCCCGCCGGTTCCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGccc                                                                                                      <  6:64176/83‑1 (MQ=255)
                                                                                                              ccgcACCAATCACCAGCAGAATGATACCCGCCGGTTCCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGccc                                                                                                      >  5:64180/1‑83 (MQ=255)
                                                                                                               cgcACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGa                                   <  8:209298/149‑1 (MQ=255)
                                                                                                                gcACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGc                                                                <  1:292294/119‑1 (MQ=255)
                                                                                                                gcACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGc                                                                >  2:292294/1‑119 (MQ=255)
                                                                                                                    cAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAAc                              >  4:110703/1‑149 (MQ=255)
                                                                                                                         aCCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCaaa                         >  4:48675/1‑149 (MQ=255)
                                                                                                                         aCCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCaaa                         <  1:200080/149‑1 (MQ=255)
                                                                                                                         aCCAGCAGAATGATCCCCGCAGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCaaa                         <  8:308104/149‑1 (MQ=255)
                                                                                                                          ccAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAAc                        <  2:178351/149‑1 (MQ=255)
                                                                                                                           cagcagAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAAc                        >  8:233847/1‑148 (MQ=255)
                                                                                                                             gcagAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCAt                     >  7:254892/1‑149 (MQ=255)
                                                                                                                              cagAATGATCCCCGCCGGTTGCAGCGCGTGCCCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGAAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTc                   >  3:131465/1‑149 (MQ=255)
                                                                                                                               agAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTc                   <  1:235261/149‑1 (MQ=255)
                                                                                                                                   tGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGt                 >  8:60997/1‑147 (MQ=255)
                                                                                                                                     aTCCTCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTaa               <  7:373078/147‑1 (MQ=255)
                                                                                                                                     aTCCTCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTaa               >  8:373078/1‑147 (MQ=255)
                                                                                                                                        cccGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGt          <  8:302249/149‑1 (MQ=255)
                                                                                                                                           gccgGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGa       <  4:303134/149‑1 (MQ=255)
                                                                                                                                           gccgGTGGCAGCGCGTGACCGCAAATCACCATCACTTTGTCTATTGGCTTGCCCTGCCGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATATACTCAAACCATTCGTAAGCAGTTGa       <  4:275585/149‑1 (MQ=255)
                                                                                                                                                ttGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCtt  >  2:133824/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AGCTGCCAGCACGAAGCAGGTGATAGCAATCGGCAGGCCCATGCCGGTTAACGCTTCGCCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTT  >  NC_000913/3576015‑3576307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: