Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,081,323 C→T 5.2% A180T (GCC→ACC)  fdoI ← formate dehydrogenase‑O, cytochrome b556 subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,081,3230CT 5.2% 243.6 / 2.3 77A180T (GCC→ACC) fdoIformate dehydrogenase‑O, cytochrome b556 subunit
Reads supporting (aligned to +/- strand):  ref base C (35/38);  new base T (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

GTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAATAAGGACGCCAGATAAT  >  NC_000913/4081176‑4081470
                                                                                                                                                   |                                                                                                                                                   
gTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCg                                                                                                                                                    >  8:201623/1‑149 (MQ=255)
     tttCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAAt                                                                                                                                               <  4:205978/149‑1 (MQ=255)
         tcGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATc                                                                                                                                              >  4:304276/1‑146 (MQ=255)
         tcGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATc                                                                                                                                              <  3:304276/146‑1 (MQ=255)
         tcGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTg                                                                                                                                           >  3:288275/1‑149 (MQ=255)
           gCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGt                                                                                                                                            <  8:86832/146‑1 (MQ=255)
             ttGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCtt                                                                                                                                       >  1:226350/1‑149 (MQ=255)
                aaCCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTc                                                                                                                                     <  4:288275/148‑1 (MQ=255)
                 aCCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAc                                                                                                                                   >  8:302066/1‑149 (MQ=255)
                 aCCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCATTCAc                                                                                                                                   >  6:93300/1‑149 (MQ=255)
                   ccAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCAcc                                                                                                                                  >  7:932/1‑148 (MQ=255)
                      gCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaaa                                                                                                                              <  7:302066/149‑1 (MQ=255)
                       cTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCaaa                                                                                                                              <  8:932/148‑1 (MQ=255)
                          aTCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGggc                                                                                                                          <  6:74911/149‑1 (MQ=255)
                             ttGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGggcggc                                                                                                                       >  2:309898/1‑149 (MQ=255)
                               gCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGggcgg                                                                                                                        <  8:65497/146‑1 (MQ=255)
                               gCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGgcggcg                                                                                                                      <  2:226350/148‑1 (MQ=255)
                                 gggATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCg                                                                                                                                                    <  7:223161/116‑1 (MQ=255)
                                 gggATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCg                                                                                                                                                    >  8:223161/1‑116 (MQ=255)
                                    aTTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGata                                                                                                                <  2:318150/149‑1 (MQ=255)
                                        tGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCTCCCAAAGGGCGGCGTAGATATGCa                                                                                                            <  6:133011/149‑1 (MQ=255)
                                        tGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCa                                                                                                            <  2:78264/149‑1 (MQ=255)
                                           gAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCa                                                                                                         <  1:63203/149‑1 (MQ=255)
                                              tACTCATTCAGCGTTCTTTTCAGTTGGCTTGGGGACCTCACGGTACCAGCGCGGGTGTGGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCACAGGGCGGCGTAGATATGCACCATg                                                                                                       <  6:168482/148‑1 (MQ=255)
                                                cTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGGTGCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGCTAATCGGGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATa                                                                                                    <  1:252131/149‑1 (MQ=255)
                                                 tCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATaa                                                                                                   >  6:68436/1‑149 (MQ=255)
                                                    ttCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACca                                                                                                <  7:201623/149‑1 (MQ=255)
                                                        gCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAAt                                                                                            <  3:338196/149‑1 (MQ=255)
                                                          tttctttTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAAtt                                                                                           >  1:248238/1‑148 (MQ=255)
                                                                  ccGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACt                                                                                  >  4:15170/1‑149 (MQ=255)
                                                                        tCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGc                                                                             >  8:302450/1‑148 (MQ=255)
                                                                          ttGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCCCCATGGCGGTAATCGTGCCGTTCACCCCAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCaaa                                                                          >  2:298965/1‑149 (MQ=255)
                                                                            gCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAaat                                                                         <  5:68436/148‑1 (MQ=255)
                                                                            gCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAaat                                                                         <  2:248238/148‑1 (MQ=255)
                                                                             cGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGGCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGAGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAaatga                                                                       <  7:137926/149‑1 (MQ=255)
                                                                             cGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAaatga                                                                       <  7:174830/149‑1 (MQ=255)
                                                                                  cTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTTCGGCAAATGAATgca                                                                  <  8:57324/149‑1 (MQ=255)
                                                                                    cACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATgcag                                                                 >  8:266474/1‑148 (MQ=255)
                                                                                         tACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCAGTac                                                           >  5:294812/1‑148 (MQ=255)
                                                                                           ccAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAAc                                                          <  5:291251/148‑1 (MQ=255)
                                                                                           ccAGCGCGGGTGATGGTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCATCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAAc                                                          <  3:130179/148‑1 (MQ=255)
                                                                                            cAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAAcgc                                                        >  1:111355/1‑149 (MQ=255)
                                                                                            cAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAAcgc                                                        >  4:41018/1‑149 (MQ=255)
                                                                                            cAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAAACACAATTAACGCCACTGCGGCaaa                                                                          >  4:200233/1‑131 (MQ=255)
                                                                                                    gTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGa                                                <  7:266474/149‑1 (MQ=255)
                                                                                                        tGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCAc                                            >  5:106040/1‑149 (MQ=255)
                                                                                                        tGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCAc                                            >  4:210502/1‑149 (MQ=255)
                                                                                                         gTTTCTTCGCCCAGGCGCTGGTACCCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTACATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCAcc                                           >  1:67443/1‑149 (MQ=255)
                                                                                                         gTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCAcc                                           >  2:18960/1‑149 (MQ=255)
                                                                                                          tttCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCg                                          <  7:302450/149‑1 (MQ=255)
                                                                                                              ttcGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGAt                                      >  2:1608/1‑149 (MQ=255)
                                                                                                              ttcGCCCAGGCGCGGGAAACCCATCCTTCCACCCTGGCGGTAATCGGGCCTTTCCCCCAACGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCgg                                         <  6:187392/146‑1 (MQ=255)
                                                                                                               tcGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATc                                                                                                                                              <  1:186770/44‑1 (MQ=255)
                                                                                                               tcGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATc                                                                                                                                              >  2:186770/1‑44 (MQ=255)
                                                                                                                cGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGAt                                      <  1:1608/147‑1 (MQ=255)
                                                                                                                 gaccAGGAGCTGGTAACCCATCCTTCAACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGAGTAGATATGCACCATGATAACCACAATTAACGCCACTGAGGCAAATGAATGCAGCATTAAAGCGAATCGGATCACCGGGATTg                                    <  5:93300/146‑1 (MQ=255)
                                                                                                                   ccAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTgaga                                 <  1:309898/149‑1 (MQ=255)
                                                                                                                         gcTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAg                           <  6:106040/149‑1 (MQ=255)
                                                                                                                         gcTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAg                           >  5:104364/1‑149 (MQ=255)
                                                                                                                           tGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGgc                         >  2:111213/1‑149 (MQ=255)
                                                                                                                              tAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGACTCCGATCACCGGGATTGAGAAAGCAGgcgc                       >  6:206226/1‑148 (MQ=255)
                                                                                                                                 cccATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCCCCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAACGCAGGCGCaaaa                   >  2:331794/1‑149 (MQ=255)
                                                                                                                                  ccATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCaaaa                   >  8:26830/1‑148 (MQ=255)
                                                                                                                                  ccATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCAAAATTAACGCCACTGCGGCAAATGAATGCATCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCaaaa                   >  2:268412/1‑148 (MQ=255)
                                                                                                                                  ccATCCTTCCACCATGGCGGTAATCGAGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAAt                  <  4:273158/149‑1 (MQ=255)
                                                                                                                                    aTCCTTCCACCATGGTGGTAATCGTTCATTTCACCCAAATGGCGGCGTAGATATGCACAATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGAGTGAGAAAGCAGGCGCaaaaa                  >  4:295149/1‑146 (MQ=255)
                                                                                                                                    aTCCTTCCACCATGGTGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAAt                  >  4:290084/1‑147 (MQ=255)
                                                                                                                                          ccaccaTGGTTGTAATCGTGCCTTTCACACAAAGTGCGGCGTAGATATGCAACATGATAACCACAATTAACGCAACTGCGGCAAATGAATTAAGCATTAAAGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAATAAGGAc            <  3:295149/147‑1 (MQ=255)
                                                                                                                                          ccaccaTGGTGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAATAAGGAc            <  3:290084/147‑1 (MQ=255)
                                                                                                                                              caTGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCTTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAATAAGGACGCCAGa      <  3:210502/149‑1 (MQ=255)
                                                                                                                                              caTGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAATAAGGACGc          >  2:171093/1‑145 (MQ=255)
                                                                                                                                                tGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAATAAGGACGCCAGAt     >  3:22890/1‑148 (MQ=255)
                                                                                                                                                  gCGGTACTCGTGCCGTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGTGATTGAGAAAGCAGGCGCAAAATAAGTACGc          <  6:266543/141‑1 (MQ=255)
                                                                                                                                                  gCGGTAATCGTGCCTTTCACCCACCGGGCGGCGTAGATATGCACCATGATAACCACAATTACCGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGAGCACCGGGATTGAGAAAGCAGGCGCAAAATAATGACGAAAGATa  >  5:266543/1‑147 (MQ=255)
                                                                                                                                                  gCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGAt                                                                                                     <  2:329082/50‑1 (MQ=255)
                                                                                                                                                  gCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGAt                                                                                                     >  1:329082/1‑50 (MQ=255)
                                                                                                                                                  gCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAATAAGGACGCCAGATAAt  <  2:111355/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTACGTTTCTCGCTTGAACCCAGCTCATCTTGCGGGATTATGCGAATACTCATTCAGCTTTCTTTTCCGTTGTCTTGCGGACCTCACGGTACCAGCGCGGGTGATGTTTCTTCGCCCAGGCGCTGGTAACCCATCCTTCCACCATGGCGGTAATCGTGCCTTTCACCCAAAGGGCGGCGTAGATATGCACCATGATAACCACAATTAACGCCACTGCGGCAAATGAATGCAGCATTAACGCGAATCGGATCACCGGGATTGAGAAAGCAGGCGCAAAATAAGGACGCCAGATAAT  >  NC_000913/4081176‑4081470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: