Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 930,357 | A→G | 6.9% | S202S (AGT→AGC) | cydD ← | glutathione/cysteine ABC transporter export permease/ATPase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 930,357 | 0 | A | G | 6.9% | 172.1 / 3.2 | 58 | S202S (AGT→AGC) | cydD | glutathione/cysteine ABC transporter export permease/ATPase |
Reads supporting (aligned to +/- strand): ref base A (19/35); new base G (2/2); total (21/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.15e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 |
CGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCA > NC_000913/930212‑930499 | cGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCa < 1:188125/146‑1 (MQ=255) cGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCa > 2:188125/1‑146 (MQ=255) cGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACt > 5:193279/1‑148 (MQ=255) gaggaTAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTaa < 8:360197/149‑1 (MQ=255) gaggaTAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTaa < 3:335683/149‑1 (MQ=255) ggaTAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGc < 5:130314/149‑1 (MQ=255) tAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCGCTTAAGc < 6:167625/146‑1 (MQ=255) tAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCGCTTAAGc > 5:167625/1‑146 (MQ=255) tAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCGCTTAAGc < 2:97326/146‑1 (MQ=255) tAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCGCTTAAGc > 1:97326/1‑146 (MQ=255) aaaCGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGc > 5:260088/1‑147 (MQ=255) aaaCGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCaa < 1:289828/149‑1 (MQ=255) aCGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAaga < 1:374322/149‑1 (MQ=255) aCGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAaga < 8:107774/149‑1 (MQ=255) gCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGACTACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGc < 6:349648/149‑1 (MQ=255) cAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCga < 3:155285/149‑1 (MQ=255) cGTAGCACTTCCATTGTCCGTTGTCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGaaa < 8:368211/149‑1 (MQ=255) aCTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACg < 2:294365/149‑1 (MQ=255) cATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACTAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCgg < 5:209955/148‑1 (MQ=255) aTTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGt > 5:360300/1‑148 (MQ=255) ttGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTa < 6:193279/149‑1 (MQ=255) tCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCa < 6:84590/149‑1 (MQ=255) cGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATc > 7:72665/1‑149 (MQ=255) ggAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGtt > 1:327846/1‑127 (MQ=255) ggAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGtt < 2:327846/127‑1 (MQ=255) ggAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCATcc > 1:100921/1‑149 (MQ=255) gAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGccc > 1:110667/1‑149 (MQ=255) aGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCa < 8:72665/149‑1 (MQ=255) gCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGAGATCGAGGAAATGCCCACTTAAGCGAGCAAGATCGAGAAAGTTACGTCGGTTAGCATCGGCAGCACCCATACCAACCAg > 8:357249/1‑149 (MQ=255) aGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAgcg > 7:308718/1‑149 (MQ=255) gAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTa > 7:49778/1‑116 (MQ=255) gAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTa < 8:49778/116‑1 (MQ=255) aCTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAaaca > 2:225841/1‑149 (MQ=255) tttCAATTTCAGCTTCACCACGCCCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACAAGCGCCATAaccaac > 7:96758/1‑149 (MQ=255) tttCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAaacaac > 7:321717/1‑149 (MQ=255) tttCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAaacaac < 1:310806/149‑1 (MQ=255) tCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAaacaac < 2:110667/147‑1 (MQ=255) tCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAaacaac < 6:260088/147‑1 (MQ=255) aTTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAAt < 8:225367/149‑1 (MQ=255) cTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGt > 8:150852/1‑149 (MQ=255) cacGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCg < 4:85528/64‑1 (MQ=255) aCCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCca < 3:168445/149‑1 (MQ=255) aCCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGGTAGCTTCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCca < 5:43459/149‑1 (MQ=255) aaTACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGaa < 7:150852/149‑1 (MQ=255) aTACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAATCAACGGAATTAACGGTGCAGTGCCCAGCAGAAt < 7:357249/149‑1 (MQ=255) cGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAg < 1:225841/149‑1 (MQ=255) aaTGTTTCCATGCCGCGCATGCGATCGATGAAATGCCCACTTAAGCGAGCAAGAGCGATAATGTTACGTCGGTTAGCATCGGCAGCCCCCATTCAAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAgc < 3:248524/147‑1 (MQ=255) aaTGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCAAGCAGAATGAgcgc > 2:250705/1‑149 (MQ=255) gcAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTagag < 8:308718/149‑1 (MQ=255) cAGGCGATCGAGGAAATGCCCACTTAATCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGACAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAgg < 8:311126/149‑1 (MQ=255) aGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAgcg > 6:260641/1‑130 (MQ=255) aGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAgcg < 5:260641/130‑1 (MQ=255) gCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAACCAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGa < 8:96758/149‑1 (MQ=255) gCGATCGAGGAAATGCCCACTAAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTACCCTCGGCAGCCCCCATTCCAACCACCGCCATAAACAACGGAATTAACGGTGCCGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGa < 5:231845/149‑1 (MQ=255) gATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAgggg > 2:200068/1‑146 (MQ=255) gATCGAGGAAATGCCCACTTAAGCAAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTagag > 4:23352/1‑143 (MQ=255) gATCGAGGAAATGCCCACTTAAGCAAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTagag < 3:23352/143‑1 (MQ=255) ggAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGcc < 6:360300/149‑1 (MQ=255) gAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGcca < 1:330332/149‑1 (MQ=255) tGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGccacca > 1:52544/1‑148 (MQ=255) | CGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCA > NC_000913/930212‑930499 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |