Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,258,755 T→A 7.0% intergenic (‑242/‑36) pth ← / → ychH peptidyl‑tRNA hydrolase/DUF2583 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,258,7550TA 7.0% 171.6 / 3.2 57intergenic (‑242/‑36)pth/ychHpeptidyl‑tRNA hydrolase/DUF2583 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base A (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TAATTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAA  >  NC_000913/1258609‑1258900
                                                                                                                                                  |                                                                                                                                                 
taatTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTcc                                                                                                                                                 >  4:124974/1‑149 (MQ=255)
 aatTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTAATTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCAGCACATAACCCACTGTGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTccc                                                                                                                                                >  3:124399/1‑149 (MQ=255)
  atTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCt                                                                                                                                               <  8:299653/149‑1 (MQ=255)
              gACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCa                                                                                                                                    <  4:20782/148‑1 (MQ=255)
               aCTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGa                                                                                                                                  <  7:319974/149‑1 (MQ=255)
                 tATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGa                                                                                                                                  >  7:78083/1‑147 (MQ=255)
                   ttAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGt                                                                                                                              >  7:119593/1‑149 (MQ=255)
                   ttAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGt                                                                                                                              >  5:60236/1‑149 (MQ=255)
                   ttAGAAAGTCAAGGTGATCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGATGTAATAGTCATCACGCCCGCTCATAACCCACTGGGTGTGGTCTATACTTTACACATAAGGCAGAGGGGTATTCCCTGTTCCAACCCAGAAAGt                                                                                                                              >  4:40936/1‑149 (MQ=255)
                      gAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATACCCTGTTACAACCCAGAAAGTTcc                                                                                                                           >  2:98644/1‑149 (MQ=255)
                         aGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCg                                                                                                                          >  3:160080/1‑147 (MQ=255)
                           tCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATACCCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAgg                                                                                                                      <  2:346600/149‑1 (MQ=255)
                           tCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCAACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCaggagg                                                                                                                      >  4:236179/1‑149 (MQ=255)
                                   ttCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATACCCTGTTACAACCCAGAAAGTTCCGGAGGTGACatat                                                                                                              >  2:173274/1‑149 (MQ=255)
                                      aGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGa                                                                                                            >  8:265159/1‑148 (MQ=255)
                                        gCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAAc                                                                                                         >  8:132541/1‑149 (MQ=255)
                                        gCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAAc                                                                                                         >  5:307521/1‑149 (MQ=255)
                                        gCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGAGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAAc                                                                                                         <  8:4988/149‑1 (MQ=255)
                                                   tAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCt                                                                                              >  5:325255/1‑149 (MQ=255)
                                                     catgtttGTTGACATAAGGGTAGTACTTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTAATCCCTGTTACAACCCAGAAAGTACCGGAGGTGACATATGAAACGCAAAAACCCTTc                                                                                            <  6:28553/145‑1 (MQ=255)
                                                     aaGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCtt                                                                                             <  1:242831/148‑1 (MQ=255)
                                                     aaGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTc                                                                                            <  7:363312/149‑1 (MQ=255)
                                                             ttgAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTc                                                                                     <  7:132541/148‑1 (MQ=255)
                                                               gAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCgg                                                                                   >  8:333808/1‑148 (MQ=255)
                                                               gAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCAGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCgg                                                                                   <  2:332037/148‑1 (MQ=255)
                                                                  aTAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAAc                                                                               <  8:78083/149‑1 (MQ=255)
                                                                     agtgTTGTAATTGTGATCACGCCCGCAGATAACCAACTGGGTGTTGTCTATACTTTACAGATAAGGAATAGGTGTATTCCCTGTTACAACCCAGAAAGTTCCGGATGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGt                                                                             <  5:38787/145‑1 (MQ=255)
                                                                            tAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATg                                                                      <  3:124974/148‑1 (MQ=255)
                                                                              aTTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATACCCTGTTACAACCCAGAAAGTTCCGGAGGGGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATggg                                                                    <  1:98644/148‑1 (MQ=255)
                                                                               ttGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGt                                                                  >  5:6165/1‑149 (MQ=255)
                                                                                 gtgATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGtt                                                                 <  6:60236/148‑1 (MQ=255)
                                                                                    aTCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGt                                                             >  4:155933/1‑149 (MQ=255)
                                                                                      cACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCt                                                           >  2:213973/1‑149 (MQ=255)
                                                                                        cGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCtgg                                                         <  7:265159/149‑1 (MQ=255)
                                                                                          cccGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCtggtg                                                       <  2:153325/149‑1 (MQ=255)
                                                                                                caTAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAAtg                                                 <  6:6165/149‑1 (MQ=255)
                                                                                                caTAACCCACTGGGTGTCGTCTATACTATACACATCACGAAGAGGGCGCATCCCTGTCACAACCCAGAAACTTCCGGAGGGGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTaaag                                                 <  6:307521/149‑3 (MQ=255)
                                                                                                 aTAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAAtgg                                                >  4:263350/1‑149 (MQ=255)
                                                                                                    aCCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAATCGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAAtggtg                                              <  3:68152/148‑1 (MQ=255)
                                                                                                     cccACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATACCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATggtggt                                            <  1:173274/149‑1 (MQ=255)
                                                                                                       cACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCg                                          >  1:272247/1‑149 (MQ=255)
                                                                                                             gtgtTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCCGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTgg                                    <  3:263350/149‑1 (MQ=255)
                                                                                                              tgttgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTggg                                   <  7:333808/149‑1 (MQ=255)
                                                                                                                   tCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTAtt                              <  3:259652/149‑1 (MQ=255)
                                                                                                                         cTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATcc                        <  1:213973/149‑1 (MQ=255)
                                                                                                                         cTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATcc                        >  4:342734/1‑149 (MQ=255)
                                                                                                                         cTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATc                         <  6:325255/148‑1 (MQ=255)
                                                                                                                          tttACTCATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCtt                                                                                             >  4:105256/1‑79 (MQ=255)
                                                                                                                          tttACTCATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCtt                                                                                             <  3:105256/79‑1 (MQ=255)
                                                                                                                               acaTAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAAc                   >  3:314659/1‑148 (MQ=255)
                                                                                                                               acaTAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGGCCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAAcc                  >  2:211245/1‑149 (MQ=255)
                                                                                                                                  tAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGt               >  4:286792/1‑149 (MQ=255)
                                                                                                                                  tAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGt               <  3:286792/149‑1 (MQ=255)
                                                                                                                                    aGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTa             >  5:310401/1‑149 (MQ=255)
                                                                                                                                         gagGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACcaca        <  4:160080/149‑1 (MQ=255)
                                                                                                                                            gggTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGtt     >  5:3081/1‑149 (MQ=255)
                                                                                                                                               tATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCACATGGGGTTGGGTCTGGTGGTAATGGTGGTCTGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTaa  >  6:341650/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TAATTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAA  >  NC_000913/1258609‑1258900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑