Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,807,094	T→C	100%	V242A (GTG→GCG)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,094	0	T	C	100.0%	254.7 / NA	71	V242A (GTG→GCG)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/38);  total (33/38)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAT  >  NC_000913/1806948‑1807236                                                                                                                                                   |                                                                                                                                               gcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATCGCGGCAAGGCCAAACGGGTTGGCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGgcgc                                                                                                                                              <  6:161906/149‑1 (MQ=255) gcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGgcgc                                                                                                                                              <  6:194668/149‑1 (MQ=255)   tGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGcca                                                                                                                                            >  6:96526/1‑149 (MQ=255)   tGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGcca                                                                                                                                            >  5:77740/1‑149 (MQ=255)     gTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGccac                                                                                                                                           >  1:336483/1‑148 (MQ=255)         aTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCg                                                                                                                                      >  7:23803/1‑149 (MQ=255)          tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCgg                                                                                                                                     <  7:120276/149‑1 (MQ=255)              gAACTCACCCAGCCGGACGATGGCCGCAAAGCCTCGCAGGAAATCTTTAATAGCGGCAAGGCAAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGGTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGaa                                                                                                                                 <  5:313708/149‑1 (MQ=255)                  tCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGGTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGc                                                                                                                             <  4:200249/149‑1 (MQ=255)                    aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGc                                                                                                                             <  1:335274/147‑1 (MQ=255)                    aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCa                                                                                                                           <  6:270826/149‑1 (MQ=255)                    aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCa                                                                                                                           <  2:281422/149‑1 (MQ=255)                      ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCa                                                                                                                           >  5:318788/1‑147 (MQ=255)                      ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCa                                                                                                                           >  1:29579/1‑147 (MQ=255)                      ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGGGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCaga                                                                                                                         <  2:231767/149‑1 (MQ=255)                             gACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGt                                                                                                                  <  8:211480/149‑1 (MQ=255)                               cGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTg                                                                                                                <  6:104843/149‑1 (MQ=255)                                   gTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGgcgc                                                                                                            >  3:291102/1‑149 (MQ=255)                                    tCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCt                                                                                                           >  8:322769/1‑149 (MQ=255)                                      cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCGCCGGTGAAAAGCCAGAGTACCGTTGGCGCTgg                                                                                                         >  7:240166/1‑149 (MQ=255)                                      cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTg                                                                                                          <  2:74564/148‑1 (MQ=255)                                      cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTg                                                                                                          <  2:172084/148‑1 (MQ=255)                                       gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGt                                                                                                        <  8:23803/149‑1 (MQ=255)                                        cAAAGCCGCGCAGGAAATCGTTAATAGCCGCAAGGCCAAACGGGTTGACGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTAAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTg                                                                                                       >  8:173373/1‑149 (MQ=255)                                         aaaGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGa                                                                                                      >  1:195887/1‑149 (MQ=255)                                         aaaGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGa                                                                                                      <  4:163050/149‑1 (MQ=255)                                           aGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACa                                                                                                    <  6:77740/149‑1 (MQ=255)                                             ccGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGCGAGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAg                                                                                                   >  4:205737/1‑148 (MQ=255)                                             ccGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAg                                                                                                   >  1:287649/1‑148 (MQ=255)                                                  cAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGt                                                                                             <  5:96526/149‑1 (MQ=255)                                                           gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                     <  4:291102/148‑1 (MQ=255)                                                           gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                    <  5:354421/149‑1 (MQ=255)                                                            ttAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGc                                                                                                  <  8:131898/134‑1 (MQ=255)                                                            ttAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGc                                                                                                  >  7:131898/1‑134 (MQ=255)                                                             tAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGa                                                                                  >  6:205672/1‑149 (MQ=255)                                                                tAGCGGCAAGGCCAAACGGGTTGTCGTTTGCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAATCTGTATTCAGGTGGCGCCACCACCGGGCAAAAGCCAGAGTCCCGTTGGCGCTGGTGCCAGAATGGTCGGCGCGATGaca                                                                                >  8:342554/1‑148 (MQ=255)                                                                     gCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGaaa                                                                          >  4:235123/1‑149 (MQ=255)                                                                           ccAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCa                                                                    <  2:29579/149‑1 (MQ=255)                                                                               aCGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATg                                                                                               >  5:23197/1‑118 (MQ=255)                                                                               aCGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATg                                                                                               <  6:23197/118‑1 (MQ=255)                                                                               aCGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGaaa                                                                <  2:195887/149‑1 (MQ=255)                                                                                   ggtGTCGTTTCCCTGGGTCCACAATGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGc                                                            <  5:217299/147‑1 (MQ=255)                                                                                    ttGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGcc                                                           <  7:342554/149‑1 (MQ=255)                                                                                      gTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCct                                                          <  2:336483/148‑1 (MQ=255)                                                                                            tCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTg                                                    >  1:93618/1‑148 (MQ=255)                                                                                              ccTGGGTCCACAAGGAGCGCTGGGTGTCGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAGATGCCTCTCTTg                                                    >  4:208600/1‑146 (MQ=255)                                                                                               cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                    <  1:249712/113‑1 (MQ=255)                                                                                               cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                    >  2:249712/1‑113 (MQ=255)                                                                                                tGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                  >  5:235715/1‑146 (MQ=255)                                                                                                   gTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATg                                            <  5:205672/149‑1 (MQ=255)                                                                                                       acaAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGaaaa                                                               <  4:214687/126‑1 (MQ=255)                                                                                                       acaAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGaaaa                                                               >  3:214687/1‑126 (MQ=255)                                                                                                       acaAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTc                                        >  1:310051/1‑149 (MQ=255)                                                                                                          tggAGCGATGGGTGATGATAGTGAAAACTGTATTCCTGTGGCGCCACCTCAGGTGATAAGCCAGCGTACGGTTGGCTCTGTTGAGAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTATTGAAGAGATGGTTCGtt                                     <  6:48850/148‑1 (MQ=255)                                                                                                          aGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGtt                                     <  2:287649/149‑1 (MQ=255)                                                                                                               cgcTGGGTGTCGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAGATGCCTCTCTTGAAGAGATGGTTCGTTTTGGc                                <  3:208600/149‑1 (MQ=255)                                                                                                                  tGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGCCAGCATGGTCGGCGCGATGCCACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGTGGGTTCGTTTTggag                               >  8:264511/1‑145 (MQ=255)                                                                                                                  tGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg                               >  3:113379/1‑147 (MQ=255)                                                                                                                          gATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                     >  7:335177/1‑149 (MQ=255)                                                                                                                           aTAGTGAAAACTGTATTCAGGTGGCGCCACCGCCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                     <  8:240166/148‑1 (MQ=255)                                                                                                                           aTAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                     <  5:55647/148‑1 (MQ=255)                                                                                                                           aTAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                     <  3:205737/148‑1 (MQ=255)                                                                                                                           aTAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                     >  1:369605/1‑148 (MQ=255)                                                                                                                           aTAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGGGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                    <  3:152611/149‑1 (MQ=255)                                                                                                                            tAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGCCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGGTTTGGCGTAGCTGCggg                     >  4:263224/1‑147 (MQ=255)                                                                                                                              gTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCCTGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGt                 <  6:318788/149‑1 (MQ=255)                                                                                                                              gTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGt                 >  8:271959/1‑149 (MQ=255)                                                                                                                               tcaaaaCTGTATACAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTCGCGCTGGTGACAGCATGGACGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTg                >  8:308830/3‑149 (MQ=255)                                                                                                                               tGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctctc                                                       <  6:120483/110‑1 (MQ=255)                                                                                                                               tGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctctc                                                       >  5:120483/1‑110 (MQ=255)                                                                                                                                gAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTg                >  7:340614/1‑148 (MQ=255)                                                                                                                                 aaaaCTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGTAGTGc               <  5:339476/148‑1 (MQ=255)                                                                                                                                           accggtggtGGCGCCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTc     >  7:358355/4‑148 (MQ=255)                                                                                                                                             cggtggtGGCGCCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAt  <  8:358355/148‑1 (MQ=255)                                                                                                                                             aGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCaa   <  7:322769/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               GCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAT  >  NC_000913/1806948‑1807236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑