Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,310,322 A→T 5.8% V28V (GTT→GTA) ‡ ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,310,3220AT 5.8% 258.0 / 3.7 81V28V (GTT→GTA) ‡adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (36/40);  new base T (2/3);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAA  >  NC_000913/2310176‑2310469
                                                                                                                                                  |                                                                                                                                                   
gCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAAc                                                                                                                                                   >  8:246451/1‑149 (MQ=255)
gCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAAc                                                                                                                                                   >  7:214224/1‑149 (MQ=255)
        tGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCATAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGc                                                                                                                                           >  2:38053/1‑149 (MQ=255)
        tGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGc                                                                                                                                           >  2:48844/1‑149 (MQ=255)
          gCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCg                                                                                                                                         >  3:259082/1‑149 (MQ=255)
          gCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCg                                                                                                                                         >  1:9559/1‑149 (MQ=255)
          gCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCg                                                                                                                                         <  1:38053/149‑1 (MQ=255)
           cTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGt                                                                                                                                        <  8:93963/149‑1 (MQ=255)
              aCAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCgg                                                                                                                                     >  2:261876/1‑149 (MQ=255)
               cggcGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGGAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGGGGCAAGGCGGGCGGCGAAAGATGCCTGTGGTACGCAGGGCGAGATCGAGTTCGGCGTCGGc                                                                                                                                    >  6:167578/4‑149 (MQ=255)
                aaCGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCa                                                                                                                                   >  5:1066/1‑149 (MQ=255)
                aaCGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCa                                                                                                                                   >  5:316748/1‑149 (MQ=255)
                   gTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCGGGCATTTGCGTAGAAGGAGACGTCTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCTAAAACGAATTCGACGTCGGCATTc                                                                                                                                <  5:167578/149‑1 (MQ=255)
                       gCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGt                                                                                                                            >  6:78959/1‑149 (MQ=255)
                         aGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcg                                                                                                                          <  6:255704/149‑1 (MQ=255)
                          ggggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgc                                                                                                                         >  8:110308/1‑149 (MQ=255)
                           gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgcg                                                                                                                        <  2:9559/149‑1 (MQ=255)
                           gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgcg                                                                                                                        >  1:294039/1‑149 (MQ=255)
                           gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgcg                                                                                                                        >  1:315138/1‑149 (MQ=255)
                           gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgcg                                                                                                                        <  2:315138/149‑1 (MQ=255)
                             gCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCggg                                                                                                                      <  2:292107/149‑1 (MQ=255)
                             gCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATGTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGAACGCACGGCGAAAACGAAATCGCCGTCGGCATTCGGGTCGCggg                                                                                                                      >  7:151417/1‑149 (MQ=255)
                              ggAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGTAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGAAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGc                                                                                                                     <  8:125604/148‑1 (MQ=255)
                                    ccAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGAc                                                                                                               <  5:82333/149‑1 (MQ=255)
                                     cAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACa                                                                                                              <  5:78959/149‑1 (MQ=255)
                                     cAGCGGCGAGTGCCGCGCTGGCAGTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACa                                                                                                              <  3:287661/149‑1 (MQ=255)
                                        cggcgTGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTTGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGAt                                                                                                           <  5:272702/149‑1 (MQ=255)
                                        cggcgAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGAt                                                                                                           <  2:294039/149‑1 (MQ=255)
                                              gTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAg                                                                                                     >  3:38720/1‑149 (MQ=255)
                                                 ccTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGt                                                                                                  <  4:239085/149‑1 (MQ=255)
                                                  cTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGtt                                                                                                 >  7:43533/1‑149 (MQ=255)
                                                     gCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGAt                                                                                              <  5:181052/149‑1 (MQ=255)
                                                       tGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGCCAGATTGCCAGCGTTGATCg                                                                                            >  4:272802/1‑149 (MQ=255)
                                                         gCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTc                                                                                          <  8:151417/149‑1 (MQ=255)
                                                          cATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCa                                                                                         <  8:109565/149‑1 (MQ=255)
                                                          cATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCa                                                                                         <  1:333951/149‑1 (MQ=255)
                                                                cGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTaa                                                                                    <  7:100269/148‑1 (MQ=255)
                                                                cGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAg                                                                                   >  8:305378/1‑149 (MQ=255)
                                                                 gTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAg                                                                                   >  2:360263/1‑148 (MQ=255)
                                                                 gTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGc                                                                                  <  7:110308/149‑1 (MQ=255)
                                                                  tAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCa                                                                                 <  1:261876/149‑1 (MQ=255)
                                                                    gAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCAtg                                                                               <  4:38720/149‑1 (MQ=255)
                                                                     aaGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCAtgt                                                                              <  1:34363/149‑1 (MQ=255)
                                                                     aaGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCAGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCAtgt                                                                              >  8:359379/1‑149 (MQ=255)
                                                                       ggAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTgg                                                                            >  2:336906/1‑149 (MQ=255)
                                                                              ttttCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCtttttt                                                                     <  7:235576/149‑1 (MQ=255)
                                                                                ttCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTc                                                                    <  6:1066/148‑1 (MQ=255)
                                                                                  cccGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCAt                                                                  <  4:259082/148‑1 (MQ=255)
                                                                                    cGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAAt                                                               <  4:300118/149‑1 (MQ=255)
                                                                                     gCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAACCGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAAt                                                               >  1:5126/1‑148 (MQ=255)
                                                                                     gCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATc                                                              <  1:4071/149‑1 (MQ=255)
                                                                                      cAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAAt                                                               >  6:254838/1‑147 (MQ=255)
                                                                                        ttcccgatCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCa                                                             <  1:35987/140‑1 (MQ=255)
                                                                                        aaGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGc                                                           <  7:246451/149‑1 (MQ=255)
                                                                                          gCATGTCTTGCGCGGCCAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGACAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTc                                                         <  2:263622/149‑1 (MQ=255)
                                                                                                   gcgcGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCa                                                             >  2:35987/1‑136 (MQ=255)
                                                                                                      cgGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGa                                             <  1:335761/149‑1 (MQ=255)
                                                                                                      cgGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGa                                             <  7:305378/149‑1 (MQ=255)
                                                                                                        gCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAATCATGTGGCTTTTTTCAAAATCAGCTCCCTGGTAAAGGATa                                           <  8:218275/149‑1 (MQ=255)
                                                                                                         cAAGACGGACGGCAAAAGATGCCTGTGCTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAg                                          >  1:360827/1‑149 (MQ=255)
                                                                                                               ggacggCATAAGATGCCTGTGGTACGCACGGCGAATACTTATTCGCCGTCGGCTTTCTGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCTTAATAAGCTCCCTGGTTAAGGATAGCCTTTa                                    <  1:115919/149‑1 (MQ=255)
                                                                                                               ggacggCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTa                                    <  1:336906/149‑1 (MQ=255)
                                                                                                                     cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAg                                               >  1:225555/1‑132 (MQ=255)
                                                                                                                     cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAg                                               <  2:225555/132‑1 (MQ=255)
                                                                                                                     cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGc                              >  2:170937/1‑149 (MQ=255)
                                                                                                                     cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGc                              >  6:161747/1‑149 (MQ=255)
                                                                                                                     cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATAGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGc                              >  4:6767/1‑149 (MQ=255)
                                                                                                                      aaaaGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGcc                             <  1:48844/149‑1 (MQ=255)
                                                                                                                      aaaaGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGcc                             >  7:246609/1‑149 (MQ=255)
                                                                                                                      aaaaGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGcc                             <  8:214224/149‑1 (MQ=255)
                                                                                                                            tGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGTTCGCGGGCTAAGACAGATTGCCAGCGTTGATAGTCAGTTAAGCATGTGTCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTc                        <  6:316748/148‑1 (MQ=255)
                                                                                                                               cTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTc                        <  7:81127/145‑1 (MQ=255)
                                                                                                                               cTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTc                        >  8:81127/1‑145 (MQ=255)
                                                                                                                                  tgGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTTGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATcc                 >  3:179118/1‑148 (MQ=255)
                                                                                                                                  tgGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCa                 >  5:37234/1‑149 (MQ=255)
                                                                                                                                     tACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACg               >  4:293968/1‑148 (MQ=255)
                                                                                                                                        cgaCGGCGAATTCGTTTTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGa                                             <  1:132760/113‑1 (MQ=255)
                                                                                                                                        cgaCGGCGAATTCGTTTTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGa                                             >  2:132760/3‑115 (MQ=255)
                                                                                                                                          aCGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCaaaaa         >  4:242145/1‑149 (MQ=255)
                                                                                                                                           cGGCGAATTCGTTTTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAAc        >  6:138713/1‑149 (MQ=255)
                                                                                                                                           cGGCGAATTCGTTTTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAAc        <  5:138713/149‑1 (MQ=255)
                                                                                                                                                 aaaCGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTGACCATCACGCAAAAACcaacaa  >  7:125453/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAA  >  NC_000913/2310176‑2310469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑