Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,339,980 G→A 5.1% P138P (CCG→CCA ubiG → bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,339,9800GA 5.1% 254.0 / 2.7 78P138P (CCG→CCAubiGbifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase
Reads supporting (aligned to +/- strand):  ref base G (41/33);  new base A (2/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCATGAT  >  NC_000913/2339839‑2340127
                                                                                                                                             |                                                                                                                                                   
gCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGcagtca                                                                                                                                               <  5:332187/148‑1 (MQ=255)
 cAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGcagtcagt                                                                                                                                             >  8:290241/1‑149 (MQ=255)
 cAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGcagtcagt                                                                                                                                             >  5:305083/1‑149 (MQ=255)
  aGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAACCATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTg                                                                                                                                            >  5:227265/1‑149 (MQ=255)
    gTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGt                                                                                                                                          >  1:341951/1‑149 (MQ=255)
       gCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGt                                                                                                                                          >  6:156085/1‑146 (MQ=255)
       gCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGt                                                                                                                                          >  1:62992/1‑146 (MQ=255)
        cAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCaga                                                                                                                                      <  5:308746/149‑1 (MQ=255)
        cAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCa                                                                                                                                        >  1:31558/1‑147 (MQ=255)
         aaaaCTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCagag                                                                                                                                     >  5:141700/1‑149 (MQ=255)
         aaaaCTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCACAGTCAGTGGTCagag                                                                                                                                     >  2:1400/1‑149 (MQ=255)
                cacgcacTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTgc                                                                                                                              <  8:1917/149‑1 (MQ=255)
                 acgcacTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTgcg                                                                                                                             >  2:359299/1‑149 (MQ=255)
                     acTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAAc                                                                                                                         >  8:187347/1‑149 (MQ=255)
                         gAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTg                                                                                                                       <  3:326923/147‑1 (MQ=255)
                              gggCATTCAGGGGGATTACGTGAAGGAAACCTTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAAc                                                                                                                <  6:227265/148‑1 (MQ=255)
                                 cATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAg                                                                                                             >  7:259695/1‑149 (MQ=255)
                                   ttCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAggc                                                                                                           >  5:268144/1‑149 (MQ=255)
                                   atCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAggc                                                                                                           >  5:104868/2‑149 (MQ=255)
                                      aGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCACAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAggcggc                                                                                                        >  2:71611/1‑149 (MQ=255)
                                        gTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGa                                                                                                      <  2:31558/149‑1 (MQ=255)
                                         tGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGAt                                                                                                     <  7:290241/149‑1 (MQ=255)
                                             ttACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAAcc                                                                                                               >  7:34953/1‑135 (MQ=255)
                                             ttACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAAcc                                                                                                               <  8:34953/135‑1 (MQ=255)
                                              tACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCt                                                                                                 <  7:187347/148‑1 (MQ=255)
                                                cGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCCGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGGCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCtttt                                                                                              <  4:253348/149‑1 (MQ=255)
                                                 gTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCttttt                                                                                             >  5:336086/1‑149 (MQ=255)
                                                    cAGCAACCCGCGGAAGAGCACGCGGCAATACATGCCGGGCAGTAGGTTGTGGTGACCTGCAGGGAGATGCTGGAGCACGGCCCGGATGCGCAGTCTGTGGGCAGAGGCTGTGCGCAAGTGGTGATACCAGGCGGCGATGTCTTTTtct                                                                                           <  6:98129/148‑1 (MQ=255)
                                                      ggAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGa                                                                                        <  2:341951/149‑1 (MQ=255)
                                                      ggAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGa                                                                                        >  5:289045/1‑149 (MQ=255)
                                                       gAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGca                                                                                                                                                   <  2:169128/89‑1 (MQ=255)
                                                       gAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGca                                                                                                                                                   >  1:169128/1‑89 (MQ=255)
                                                       gAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGac                                                                                       <  5:156085/149‑1 (MQ=255)
                                                         aaCCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGGCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGacac                                                                                     <  4:89279/149‑1 (MQ=255)
                                                           ccGGGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCGTCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGGCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACtt                                                                                   <  2:172658/149‑1 (MQ=255)
                                                            cGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACtt                                                                                   >  7:46788/1‑148 (MQ=255)
                                                             gTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTaa                                                                                 >  8:346665/1‑149 (MQ=255)
                                                                gAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCg                                                                              >  8:26040/1‑149 (MQ=255)
                                                                         gcgGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGc                                                                       >  8:227390/1‑147 (MQ=255)
                                                                             cAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCa                                                                 >  4:132104/1‑149 (MQ=255)
                                                                              aaaaCATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCAt                                                                >  7:199069/1‑149 (MQ=255)
                                                                                aaCATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCACAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATgg                                                              <  1:1400/149‑1 (MQ=255)
                                                                                    tGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGa                                                          >  3:266027/1‑149 (MQ=255)
                                                                                     gCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGTTGAt                                                         >  8:257247/1‑149 (MQ=255)
                                                                                     gCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGAt                                                         >  3:241096/1‑149 (MQ=255)
                                                                                       cGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCa                                                                                                              >  4:274133/1‑94 (MQ=255)
                                                                                       cGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCa                                                                                                              <  3:274133/94‑1 (MQ=255)
                                                                                       cGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATgg                                                       >  8:179502/1‑149 (MQ=255)
                                                                                              tATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATgg                                                              <  2:50405/135‑1 (MQ=255)
                                                                                              tATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATgg                                                              >  1:50405/1‑135 (MQ=255)
                                                                                                tgatgTTGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATTTCTTTTTCTCGACTCTTAACCGCAACGGCAATTCATGGCTGATGGCGgttgtt                                               <  8:129048/148‑1 (MQ=255)
                                                                                                 gatgTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTaa                                                                                 <  3:82370/113‑1 (MQ=255)
                                                                                                 gatgTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTaa                                                                                 >  4:82370/1‑113 (MQ=255)
                                                                                                 gatgTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggttgg                                             >  8:194239/1‑149 (MQ=255)
                                                                                                  atgTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCACAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggttgg                                             <  1:71611/148‑1 (MQ=255)
                                                                                                       gtgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCgg                                        >  7:154152/1‑148 (MQ=255)
                                                                                                       gtgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGa                                       <  4:266027/149‑1 (MQ=255)
                                                                                                          aCCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAata                                    <  6:305083/149‑1 (MQ=255)
                                                                                                               cATGGAGTTTCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGTGCCTGTGCGAAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTATCCGCAACGGCAAGTCTTGGCTGATGGCGGTGGTTGGTGCGGAATATAttt                               <  4:38286/149‑1 (MQ=255)
                                                                                                               cATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGCGCCTGTGCGCAACTGGTGAACCCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATAttt                               >  3:330737/1‑149 (MQ=255)
                                                                                                                  ggAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTgc                            >  4:95605/1‑149 (MQ=255)
                                                                                                                   gagaTGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTgcg                           >  3:124280/1‑149 (MQ=255)
                                                                                                                   gagaTGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTgc                            <  7:179502/148‑1 (MQ=255)
                                                                                                                        gCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATgg                      >  1:189472/1‑149 (MQ=255)
                                                                                                                           ggAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGGCTTTTTCTCGACACTTCACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGc                   <  6:104868/149‑1 (MQ=255)
                                                                                                                           ggAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGGCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGc                   <  6:268144/149‑1 (MQ=255)
                                                                                                                            gAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTg                    <  8:259695/147‑1 (MQ=255)
                                                                                                                            gAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGc                   <  7:371341/148‑1 (MQ=255)
                                                                                                                              gCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCttttt                                                                                             >  3:327525/1‑72 (MQ=255)
                                                                                                                              gCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCttttt                                                                                             <  4:327525/72‑1 (MQ=255)
                                                                                                                              gCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGccc                 >  6:251661/1‑148 (MQ=255)
                                                                                                                                 cGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAg             <  7:346665/149‑1 (MQ=255)
                                                                                                                                 cGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAg             <  3:132104/149‑1 (MQ=255)
                                                                                                                                  gTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAgg            >  4:86654/1‑149 (MQ=255)
                                                                                                                                     cccGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGTTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCa          <  7:257247/148‑1 (MQ=255)
                                                                                                                                     cccGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCAc         >  1:112837/1‑149 (MQ=255)
                                                                                                                                     cccGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTATATTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCAc         >  2:352383/1‑149 (MQ=255)
                                                                                                                                            cGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCatgat  <  6:336086/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATGGTGCCCAAAGGCACGCATGAT  >  NC_000913/2339839‑2340127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: