Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,577,513 G→A 5.9% P289S (CCG→TCG)  maeB ← malic enzyme: putative oxidoreductase/phosphotransacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,577,5130GA 5.9% 214.0 / 2.2 67P289S (CCG→TCG) maeBmalic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base G (28/35);  new base A (2/2);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCA  >  NC_000913/2577365‑2577658
                                                                                                                                                    |                                                                                                                                                 
gCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATttccggttccgg                                                                                                                                                   <  1:156453/149‑1 (MQ=255)
gCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATttccggttccgg                                                                                                                                                   <  7:69657/149‑1 (MQ=255)
 cGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCggg                                                                                                                                                  <  8:141763/149‑1 (MQ=255)
   gTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCAAAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGtt                                                                                                                                                >  8:375615/1‑149 (MQ=255)
    ttGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTc                                                                                                                                               <  4:74441/149‑1 (MQ=255)
    ttGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTc                                                                                                                                               <  3:138526/149‑1 (MQ=255)
    ttGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTc                                                                                                                                               <  8:10109/149‑1 (MQ=255)
        gcCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTcgcca                                                                                                                                           >  8:145288/1‑149 (MQ=255)
        gcCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTcgc                                                                                                                                             >  2:344435/1‑147 (MQ=255)
        gcCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTc                                                                                                                                               <  1:287181/145‑1 (MQ=255)
        gcCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTc                                                                                                                                               <  1:237309/145‑1 (MQ=255)
        gcCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTc                                                                                                                                               >  2:287181/1‑145 (MQ=255)
        gcCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTc                                                                                                                                               >  2:237309/1‑145 (MQ=255)
          cAACGTCCAGCGCGCCACGGAAGATGAACGGGGAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTACGGTTCCGGGTTcgccagc                                                                                                                                         >  8:336662/1‑149 (MQ=255)
           aaCGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTcgccagcg                                                                                                                                        <  2:90724/149‑1 (MQ=255)
               tCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTcgccagcgccag                                                                                                                                    <  5:31457/149‑1 (MQ=255)
                 cAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGAGTTCGCCCGCGCCAgg                                                                                                                                   >  2:139612/1‑148 (MQ=255)
                 cAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGAGTTCGCCAGCGCCAGGa                                                                                                                                  >  2:144763/1‑149 (MQ=255)
                  aGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGAt                                                                                                                                 <  1:17342/149‑1 (MQ=255)
                     gcgcCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCAt                                                                                                                              <  8:91060/149‑1 (MQ=255)
                     gcgcCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCAt                                                                                                                              >  1:323433/1‑149 (MQ=255)
                       gcCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTg                                                                                                                            <  2:71709/149‑1 (MQ=255)
                         cACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGgc                                                                                                                          >  8:146459/1‑149 (MQ=255)
                               aGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGa                                                                                                                    <  7:375615/149‑1 (MQ=255)
                               aGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGa                                                                                                                    <  7:275287/149‑1 (MQ=255)
                                 aTGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGc                                                                                                                  <  7:357431/149‑1 (MQ=255)
                                   gAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCa                                                                                                                >  1:287055/1‑149 (MQ=255)
                                       gggAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCAtttt                                                                                                            <  8:164435/149‑1 (MQ=255)
                                        ggAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTttc                                                                                                           <  8:248166/149‑1 (MQ=255)
                                            gCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCa                                                                                                       <  4:211096/149‑1 (MQ=255)
                                              acaGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCAc                                                                                                      >  3:25530/1‑148 (MQ=255)
                                              acaGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCAc                                                                                                      >  8:69211/1‑148 (MQ=255)
                                               caGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTAACCa                                                                                                    >  7:190884/1‑149 (MQ=255)
                                                aGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATTGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCAt                                                                                                   >  1:85003/1‑149 (MQ=255)
                                                  gACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCAttt                                                                                                 >  2:200981/1‑149 (MQ=255)
                                                     gttgttCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCt                                                                                              <  7:325790/149‑1 (MQ=255)
                                                      ttgttCACCTGGTTCGGATAGGCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTg                                                                                             <  5:137264/149‑1 (MQ=255)
                                                        gttCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTgg                                                                                            <  7:69211/148‑1 (MQ=255)
                                                              cTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCa                                                                                     >  7:220682/1‑149 (MQ=255)
                                                                             aGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCAttt                                                                                                 <  5:256039/122‑1 (MQ=255)
                                                                             aGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCAttt                                                                                                 >  6:256039/1‑122 (MQ=255)
                                                                              gAACGACCGGGGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACa                                                                     <  2:17551/149‑1 (MQ=255)
                                                                                   aCCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCa                                                                <  7:372604/149‑1 (MQ=255)
                                                                                     cGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAgg                                                              <  7:336448/149‑1 (MQ=255)
                                                                                     cGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAgg                                                              <  1:200981/149‑1 (MQ=255)
                                                                                     cGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGAGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAgg                                                              <  1:139612/149‑1 (MQ=255)
                                                                                     cGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGAGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAgg                                                              <  1:144763/149‑1 (MQ=255)
                                                                                          cAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAat                                                         <  7:145288/149‑1 (MQ=255)
                                                                                             atgatgGCATCCGTACGCACTTCTTTCGCCAGCGCCGGCAGAATTACCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGACGGAACAGCCCAGGAAAATATc                                                      >  8:168616/1‑149 (MQ=255)
                                                                                              tgatgGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATcc                                                     <  5:299280/149‑1 (MQ=255)
                                                                                              tgatgGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATcc                                                     <  8:220682/149‑1 (MQ=255)
                                                                                                 tgGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGGCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCcgc                                                   <  1:344435/148‑1 (MQ=255)
                                                                                                     aTCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAg                                                               >  4:146769/1‑132 (MQ=255)
                                                                                                         ggACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAAt                                          >  5:193329/1‑149 (MQ=255)
                                                                                                          gACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCgg                                                                         >  7:107460/1‑117 (MQ=255)
                                                                                                          gACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCgg                                                                         <  8:107460/117‑1 (MQ=255)
                                                                                                          ctacatcttcttTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAAt                                          <  3:248805/142‑1 (MQ=255)
                                                                                                            cGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATcac                                       <  2:85003/149‑1 (MQ=255)
                                                                                                               aCTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCAcat                                     >  8:31645/1‑148 (MQ=255)
                                                                                                                 ttcttTCGCCAGCGGCGGCCTAATTTCCGGTGCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCAcatcat                                  <  6:19429/149‑1 (MQ=255)
                                                                                                                 ttcttTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCCCCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCAcatcat                                  >  5:207311/1‑149 (MQ=255)
                                                                                                                   cttTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACatcatc                                 >  7:10822/1‑148 (MQ=255)
                                                                                                                       cGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAgg                             <  2:26585/148‑1 (MQ=255)
                                                                                                                         ccAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTa                          >  2:128931/1‑149 (MQ=255)
                                                                                                                         ccAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTa                          >  8:90614/1‑149 (MQ=255)
                                                                                                                            gcggcgGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGt                       <  7:31645/149‑1 (MQ=255)
                                                                                                                                cggcAGAATTTCCGTTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGACTTCAATCACATCATCGAGGGTACGTTTGc                   <  3:294689/149‑1 (MQ=255)
                                                                                                                                   cAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGt                >  3:34845/1‑149 (MQ=255)
                                                                                                                                   cAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGt                >  1:239844/1‑149 (MQ=255)
                                                                                                                                     gAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCa              >  7:12133/1‑149 (MQ=255)
                                                                                                                                         ttccggttccggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATcca          >  6:186389/1‑149 (MQ=255)
                                                                                                                                                  cggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCa  >  1:120348/1‑148 (MQ=255)
                                                                                                                                                  cggGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCa  >  6:148740/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTCGCCAGCGGCGGCAGAATTTCCGGTTCCGGGTTCGCCAGCGCCAGGATCATTGGCGCACGAGCCATTTTCTTCACCATTTCCTGGGTCAGCACTTTCGGGCCGGAACAGCCCAGGAAAATATCCGCGCCTTCAATCACATCATCGAGGGTACGTTTGCCGTCATCCACCACCGCA  >  NC_000913/2577365‑2577658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: