Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,734,830 G→A 5.1% Q69* (CAG→TAG)  yfiH ← UPF0124 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,734,8300GA 5.1% 253.3 / 2.6 78Q69* (CAG→TAG) yfiHUPF0124 family protein
Reads supporting (aligned to +/- strand):  ref base G (35/39);  new base A (2/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

CGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGG  >  NC_000913/2734687‑2734976
                                                                                                                                               |                                                                                                                                                  
cGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGAGTATTGCTATAAGAGGCATCCGCCCGTTTTGATGCATAAGGTTCGCCAGTTAGCTTAAGCACATCTTTGCCGTGTACCTGCTCaa                                                                                                                                               <  7:157056/149‑1 (MQ=255)
         gCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCg                                                                                                                                      >  7:375686/1‑149 (MQ=255)
          cAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCgg                                                                                                                                     >  6:16180/1‑149 (MQ=255)
          cAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCgg                                                                                                                                     >  4:46891/1‑149 (MQ=255)
            aaaCAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGtt                                                                                                                                   <  5:344214/149‑1 (MQ=255)
            aaaCAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGtt                                                                                                                                   <  5:120157/149‑1 (MQ=255)
                aGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGa                                                                                                                               <  1:329770/149‑1 (MQ=255)
                   acaGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAgg                                                                                                                            >  4:344490/1‑149 (MQ=255)
                    caGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTACTCAAGCCAGACCGGTTTAGAAgg                                                                                                                            >  8:178083/1‑148 (MQ=255)
                    caGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTATGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTACTCAAGCCAGACCGCTTTAGAAgg                                                                                                                            >  2:11605/1‑148 (MQ=255)
                       ggAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCaaa                                                                                                                        <  2:339300/149‑1 (MQ=255)
                            cagtcggcagtcATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGcc                                                                                                                   <  6:348748/149‑1 (MQ=255)
                             agtcggcagtcATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGccc                                                                                                                  >  8:206329/1‑149 (MQ=255)
                              gtcggcagtcATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCg                                                                                                                 >  3:57092/1‑149 (MQ=255)
                               tcggcagtcATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGc                                                                                                                           <  4:179812/138‑1 (MQ=255)
                               tcggcagtcATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGc                                                                                                                           >  3:179812/1‑138 (MQ=255)
                                 ggcagtcATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGATTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCgca                                                                                                               <  3:218509/148‑1 (MQ=255)
                                 ggcaggcaTCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCgca                                                                                                               <  4:157728/148‑1 (MQ=255)
                                    agtcATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAGGCCAGACCGGTTTAGAAGGCAAATTGCCCgcagca                                                                                                            >  4:350713/1‑148 (MQ=255)
                                      tcatcaCTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCaaa                                                                                                          >  5:319942/1‑148 (MQ=255)
                                        atcaCTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAg                                                                                                       >  2:254875/1‑149 (MQ=255)
                                           aCTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCg                                                                                                    >  8:153564/1‑149 (MQ=255)
                                             tGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGc                                                                                                                                                   <  1:200730/100‑1 (MQ=255)
                                             tGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGc                                                                                                                                                   >  2:200730/1‑100 (MQ=255)
                                                     ccGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATtc                                                                                          <  7:5409/149‑1 (MQ=255)
                                                     ccGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATtc                                                                                          <  4:77288/149‑1 (MQ=255)
                                                             ggCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAAc                                                                                  <  7:252478/149‑1 (MQ=255)
                                                                gTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTg                                                                               <  7:206329/149‑1 (MQ=255)
                                                                  aTTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGAt                                                                             >  1:299975/1‑149 (MQ=255)
                                                                     gCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGa                                                                                             >  6:175732/1‑130 (MQ=255)
                                                                     gCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGa                                                                                             <  5:175732/130‑1 (MQ=255)
                                                                      cTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAAtt                                                                                                                      <  3:319820/104‑1 (MQ=255)
                                                                      cTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAAtt                                                                                                                      >  4:319820/1‑104 (MQ=255)
                                                                      cTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACTTGATCCgg                                                                         >  6:200090/1‑149 (MQ=255)
                                                                       tataAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAAtt                                                                                                                      >  3:137108/1‑103 (MQ=255)
                                                                       tataAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAAtt                                                                                                                      <  4:137108/103‑1 (MQ=255)
                                                                         taAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGtt                                                                      <  2:299975/149‑1 (MQ=255)
                                                                         taAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGt                                                                       >  5:281258/1‑148 (MQ=255)
                                                                         taAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGt                                                                       >  1:339744/1‑148 (MQ=255)
                                                                               gCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGcc                                                                <  1:78854/149‑1 (MQ=255)
                                                                                 aTCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATc                                                                   <  5:166053/144‑1 (MQ=255)
                                                                                 aTCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATc                                                                   >  6:166053/1‑144 (MQ=255)
                                                                                  tCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTAt                                                                    <  6:117412/142‑1 (MQ=255)
                                                                                  tCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTAt                                                                    >  5:117412/1‑142 (MQ=255)
                                                                                  tCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTGATCGCcac                                                              >  1:259602/1‑148 (MQ=255)
                                                                                    cGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACaa                                                            <  6:319942/148‑1 (MQ=255)
                                                                                    cGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTAAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTACCGCCACaa                                                            <  5:110624/148‑1 (MQ=255)
                                                                                    cGCCCGTGTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAAt                                                           <  1:353837/149‑1 (MQ=255)
                                                                                     gCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATg                                                          <  3:277607/149‑1 (MQ=255)
                                                                                     gCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATg                                                          >  2:303325/1‑149 (MQ=255)
                                                                                     gCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATg                                                          >  4:277607/1‑149 (MQ=255)
                                                                                        cGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGc                                                       <  7:153564/149‑1 (MQ=255)
                                                                                              gAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGt                                                                                <  5:2845/118‑1 (MQ=255)
                                                                                              gAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGt                                                                                >  6:2845/1‑118 (MQ=255)
                                                                                               aGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGGTTAGCAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAgg                                                >  8:110980/1‑149 (MQ=255)
                                                                                                    tAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGGTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTtgag                                           >  1:359204/1‑149 (MQ=255)
                                                                                                      aGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTACTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTtgagt                                          <  7:178083/148‑1 (MQ=255)
                                                                                                      aGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTACTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGGCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTtgagt                                          <  1:11605/148‑1 (MQ=255)
                                                                                                        gTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGt                                                                                                                                    <  4:28920/56‑1 (MQ=255)
                                                                                                        gTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTtgagtgag                                       >  4:333950/1‑149 (MQ=255)
                                                                                                        gTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTtgagtgag                                       <  4:57092/149‑1 (MQ=255)
                                                                                                         ttCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTgagtgagt                                      >  1:336742/1‑149 (MQ=255)
                                                                                                               aGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACg                                >  7:66380/1‑149 (MQ=255)
                                                                                                                gTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGTCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACgg                               >  8:326444/1‑149 (MQ=255)
                                                                                                                         aGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGc                       <  2:336742/148‑1 (MQ=255)
                                                                                                                         aGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCt                      >  5:52862/1‑149 (MQ=255)
                                                                                                                          gCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCt                      >  2:372430/1‑148 (MQ=255)
                                                                                                                           cacaTCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCa                    <  7:112972/149‑1 (MQ=255)
                                                                                                                           cacaTCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCa                    <  7:277295/149‑1 (MQ=255)
                                                                                                                            acaTCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCa                    <  7:326444/148‑1 (MQ=255)
                                                                                                                            acaTCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCAc                   >  5:265248/1‑149 (MQ=255)
                                                                                                                                   tGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCAcgccgc              <  1:254875/147‑1 (MQ=255)
                                                                                                                                    gCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGccgccg            <  5:200090/148‑1 (MQ=255)
                                                                                                                                      cGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATa         <  3:344490/149‑1 (MQ=255)
                                                                                                                                       gtgtACCTGCTCAAGCCAGACGGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGATGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATAc        <  6:281258/149‑1 (MQ=255)
                                                                                                                                        tgtACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATAc        >  5:223974/1‑148 (MQ=255)
                                                                                                                                          tACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGa      <  6:223974/148‑1 (MQ=255)
                                                                                                                                             cTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTgg  <  2:339744/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGG  >  NC_000913/2734687‑2734976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑