Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,800,662 A→G 5.2% intergenic (+187/‑61) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,800,6620AG 5.2% 243.4 / 2.3 77intergenic (+187/‑61)ygaM/nrdHputative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
Reads supporting (aligned to +/- strand):  ref base A (42/31);  new base G (2/2);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGATTTTGAAA  >  NC_000913/2800514‑2800810
                                                                                                                                                    |                                                                                                                                                    
gCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACa                                                                                                                                                      <  1:113141/149‑1 (MQ=255)
  cTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACaga                                                                                                                                                    <  8:307572/149‑1 (MQ=255)
   tGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACa                                                                                                                                                      >  5:238466/1‑146 (MQ=255)
     tCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACaga                                                                                                                                                    <  6:238466/146‑1 (MQ=255)
         cGGGGTTTCTATTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGCGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGAc                                                                                                                                               <  7:377557/147‑1 (MQ=255)
          ggggTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACaga                                                                                                                                                    <  5:307019/141‑1 (MQ=255)
          ggggTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACaga                                                                                                                                                    >  6:307019/1‑141 (MQ=255)
          ggggTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAAc                                                                                                                                            >  3:341011/1‑149 (MQ=255)
                  tttttGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAGCTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGAcgcg                                                                                                                                    >  7:92452/1‑149 (MQ=255)
                      tGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTat                                                                                                                                 <  6:107761/148‑1 (MQ=255)
                         cTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCTACGCGTatcatc                                                                                                                             <  8:4414/149‑1 (MQ=255)
                            gTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTATTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATcgcg                                                                                                                          >  3:212932/1‑149 (MQ=255)
                            gTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATcgcg                                                                                                                          >  8:315787/1‑149 (MQ=255)
                                 tCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGTCAACCCGACGCGTATCATCGCGCCGTa                                                                                                                     >  3:13337/1‑149 (MQ=255)
                                         aTCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCAtt                                                                                                             <  2:96004/149‑1 (MQ=255)
                                          tCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTTTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCATCAGAGAGACAACCCGACGAGTATCATCGCGCCGTATCTTCAttt                                                                                                            <  4:25560/149‑1 (MQ=255)
                                               cTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAAc                                                                                                       <  7:315787/149‑1 (MQ=255)
                                               cTACCCCGTCACGCTCATATCCAGCGTAATTTCGACCACTATTTGCAAAATATTGTGTCGTTGAATCTTTTATCAACTACATCTAGTATCACTGTATCAACAGAGCGACAACCCGACGCGTATCATCGCGCCGTATCATCATTTTAAAc                                                                                                       >  1:3820/1‑149 (MQ=255)
                                                     cGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGaaa                                                                                                  >  3:275087/1‑148 (MQ=255)
                                                       tCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATAc                                                                                               <  8:106981/149‑1 (MQ=255)
                                                           gCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAAt                                                                                           >  5:112026/1‑149 (MQ=255)
                                                             tCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCa                                                                                         >  4:190228/1‑149 (MQ=255)
                                                                  tCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATgcg                                                                                     >  3:207572/1‑148 (MQ=255)
                                                                   ccAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATATGAAtgaccgcc                                                                                    >  4:10279/1‑141 (MQ=255)
                                                                   ccAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCAAGTATCTCTGTATCAACAGAGAGAAAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACCAATCATGCGCa                                                                                   >  8:194516/1‑149 (MQ=255)
                                                                     aGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCAt                                                                                  >  8:33800/1‑148 (MQ=255)
                                                                     aGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGGATCATCGCGCCGGATCTTCATTTTAAACGGAAATACGAATCATGCGCAt                                                                                  >  2:89116/1‑148 (MQ=255)
                                                                      gggTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTa                                                                                >  3:4735/1‑149 (MQ=255)
                                                                       ggTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTAc                                                                               <  3:151530/149‑1 (MQ=255)
                                                                       ggTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGACGTATCTTCATTTTAAACGGAAATACGAATCATGAGCATTAc                                                                               >  1:154215/1‑149 (MQ=255)
                                                                            tttCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTAtt                                                                           >  6:217507/1‑148 (MQ=255)
                                                                                gACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACAGAAATACGAATCATGCGCATTACTATTTaca                                                                       <  2:219172/148‑1 (MQ=255)
                                                                                gACCACTATTTGCTATATATTGTGTGGTTGAATATTTTTTCAACTACATCTAGTATCTCTGTAGCAACAGAGAGACAACCCGACGCGTATCCTCGCGCCGGAGCTTCATTTTCAACGGAACTCCGCCGCCTGCGCATGACTATTTccac                                                                      <  2:248173/149‑1 (MQ=255)
                                                                                  ccACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAGTCATGCGCATTACTATTTACACTc                                                                    >  7:11029/1‑149 (MQ=255)
                                                                                      tATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATAGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTaa                                                                >  3:160096/1‑149 (MQ=255)
                                                                                            cTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGAtt                                                           <  6:112026/148‑1 (MQ=255)
                                                                                             tatataTTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGc                                                         >  8:362735/1‑149 (MQ=255)
                                                                                              atatatTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTa                                                                                <  6:281174/125‑1 (MQ=255)
                                                                                              atatatTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTa                                                                                >  5:281174/1‑125 (MQ=255)
                                                                                                  atTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGa                                                             <  4:203721/140‑1 (MQ=255)
                                                                                                  atTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGa                                                             >  3:203721/1‑140 (MQ=255)
                                                                                                   ttGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAGTCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCa                                                    <  8:11029/148‑1 (MQ=255)
                                                                                                   ttGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAg                                                   <  4:275087/149‑1 (MQ=255)
                                                                                                   ttGTGTGGTCGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAg                                                   <  8:81225/149‑1 (MQ=255)
                                                                                                     gtgtgGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGt                                                  <  7:362735/148‑1 (MQ=255)
                                                                                                     gtgtgGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGt                                                  <  7:15635/148‑1 (MQ=255)
                                                                                                       gtgGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTAGTAACGATTGCGTTCAGttc                                                >  1:298900/1‑146 (MQ=255)
                                                                                                          gTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTa                                                                                <  4:302847/113‑1 (MQ=255)
                                                                                                          gTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTa                                                                                >  3:302847/1‑113 (MQ=255)
                                                                                                           ttGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTgccacgc                                           >  6:196645/1‑149 (MQ=255)
                                                                                                             gAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGACTGCGTTCAGTgccacgcca                                         >  4:31759/1‑149 (MQ=255)
                                                                                                             gAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGAATTACTATTTACACTCGTAACGATTGCGTTCAGTgccacgcca                                         >  5:55004/1‑149 (MQ=255)
                                                                                                              aaTCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTgccacgccac                                        >  4:192503/1‑149 (MQ=255)
                                                                                                              aaTCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACAATTTACACTCGTAACGATTGCGTTCAGTgccacgcca                                         <  7:83471/148‑1 (MQ=255)
                                                                                                                  ttttttCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCaaa                                    >  8:174834/1‑149 (MQ=255)
                                                                                                                   tttttCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAAc                                   >  7:150604/1‑149 (MQ=255)
                                                                                                                    ttttCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCaaa                                    <  3:190228/147‑1 (MQ=255)
                                                                                                                      ttCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGATATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACggg                                >  3:260533/1‑149 (MQ=255)
                                                                                                                      ttCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACgg                                 <  4:255605/148‑1 (MQ=255)
                                                                                                                       tCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTGCAGTGCCACGCCACCAAACggg                                <  4:4735/148‑1 (MQ=255)
                                                                                                                                tCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGaaaa                      >  8:77085/1‑149 (MQ=255)
                                                                                                                                  tAGTATCTCTGTATCAACAGAGAGACAACCCGCCGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAAcc                    >  5:306683/1‑149 (MQ=255)
                                                                                                                                  tAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAAcc                    >  5:186700/1‑149 (MQ=255)
                                                                                                                                    gTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACggg                                >  8:343455/1‑135 (MQ=255)
                                                                                                                                    gTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACggg                                <  7:343455/135‑1 (MQ=255)
                                                                                                                                      aTCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTgccacgccac                                        <  2:12163/125‑1 (MQ=255)
                                                                                                                                          ctGTATCAACGGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCgggg                <  1:145220/145‑1 (MQ=255)
                                                                                                                                          ctGTATCAACGGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCgggg                <  1:150364/145‑1 (MQ=255)
                                                                                                                                          ctGTATCAACGGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCgggg                >  2:145220/1‑145 (MQ=255)
                                                                                                                                          ctGTATCAACGGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCgggg                >  2:150364/1‑145 (MQ=255)
                                                                                                                                          ctGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCttt            >  3:241707/1‑149 (MQ=255)
                                                                                                                                          ctGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCttt            >  3:48266/1‑149 (MQ=255)
                                                                                                                                           tGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCttt            >  7:31362/1‑148 (MQ=255)
                                                                                                                                           tGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCttt            >  5:54185/1‑148 (MQ=255)
                                                                                                                                             tATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGa          <  8:92452/148‑1 (MQ=255)
                                                                                                                                             tATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGAt         <  4:341011/149‑1 (MQ=255)
                                                                                                                                              aTCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGAtt        >  5:331503/1‑149 (MQ=255)
                                                                                                                                               tCAACAGAGAGTCAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGAttt       <  4:13337/149‑1 (MQ=255)
                                                                                                                                               tCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGAtt        <  6:54185/148‑1 (MQ=255)
                                                                                                                                                  aCAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCgggg                >  8:41058/1‑137 (MQ=255)
                                                                                                                                                  aCAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCgggg                <  7:41058/137‑1 (MQ=255)
                                                                                                                                                  aCAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGATTTTGa    <  6:306683/149‑1 (MQ=255)
                                                                                                                                                  aCAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTATGATTTTg     >  4:233229/1‑148 (MQ=255)
                                                                                                                                                    agagagACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGATTTTGaaa  <  6:186700/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGACAACCCGACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGCGCATTACTATTTACACTCGTAACGATTGCGTTCAGTGCCACGCCACCAAACGGGCGATGGAAAACCGGGGCTTTGATTTTGAAA  >  NC_000913/2800514‑2800810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: