Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,297	G→T	D193E (GAC→GAA)	prs ←	phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,297	0	G	T	100.0%	154.3 / NA	43	D193E (GAC→GAA)	prs	phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/21);  total (22/21)

GCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAC  >  NC_000913/1261154‑1261440                                                                                                                                                |                                                                                                                                                gCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCgat                                                                                                                                            <  3:110926/149‑1 (MQ=255)    ccacGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCgatgat                                                                                                                                         >  4:85131/1‑149 (MQ=255)     cacGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGAtt                                                                                                                                        >  7:119295/1‑149 (MQ=255)         ttCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCa                                                                                                                                    >  2:240167/1‑149 (MQ=255)           cTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCat                                                                                                                                   <  8:119295/148‑1 (MQ=255)                        agcagcTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTca                                                                                                                     <  1:240167/149‑1 (MQ=255)                        agcagcTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTc                                                                                                                      <  7:57066/148‑1 (MQ=255)                                   acaGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCat                                                                                                                                   <  6:126311/124‑1 (MQ=255)                                   acaGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCat                                                                                                                                   >  5:126311/1‑124 (MQ=255)                                         tACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATc                                                                                                                          >  5:42349/1‑127 (MQ=255)                                         tACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATc                                                                                                                          <  6:42349/127‑1 (MQ=255)                                             gCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGtt                                                                                                                       <  1:67726/126‑1 (MQ=255)                                             gCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGtt                                                                                                                       >  2:67726/1‑126 (MQ=255)                                              ccAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTc                                                                                                                                               <  1:188822/101‑1 (MQ=255)                                              ccAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTc                                                                                                                                               >  2:188822/1‑101 (MQ=255)                                              ccAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCggg                                                                                                >  2:50799/1‑148 (MQ=255)                                                aGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGgcac                                                                                             >  5:158717/1‑149 (MQ=255)                                                                           gCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGAtt                                                                                                                                        <  4:33149/79‑1 (MQ=255)                                                                           gCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGAtt                                                                                                                                        >  3:33149/1‑79 (MQ=255)                                                                           gCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGt                                                                                                                             <  7:58788/90‑1 (MQ=255)                                                                           gCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGt                                                                                                                             >  8:58788/1‑90 (MQ=255)                                                                             aGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTAt                                                                                                                           >  5:30483/1‑90 (MQ=255)                                                                             aGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTAt                                                                                                                           <  6:30483/90‑1 (MQ=255)                                                                              gTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACca                                                               >  5:148003/1‑149 (MQ=255)                                                                               tCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACca                                                               >  2:552/1‑148 (MQ=255)                                                                                 aCGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAAc                                                                 <  4:105668/144‑1 (MQ=255)                                                                                 aCGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAAc                                                                 >  3:105668/1‑144 (MQ=255)                                                                                      cTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGAACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTggg                                                       <  4:137167/149‑1 (MQ=255)                                                                                          aaCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTggg                                                       >  5:3039/1‑145 (MQ=255)                                                                                          aaCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTggg                                                       <  6:3039/145‑1 (MQ=255)                                                                                                       atatGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCt                                       <  3:85131/148‑1 (MQ=255)                                                                                                             aTCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCAt                                <  1:552/149‑1 (MQ=255)                                                                                                              tCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATg                               >  8:82510/1‑149 (MQ=255)                                                                                                                aCCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGgcacgca                                                                                          <  4:31971/88‑1 (MQ=255)                                                                                                                aCCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGgcacgca                                                                                          >  3:31971/1‑88 (MQ=255)                                                                                                                 ccTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTcc                                                                         >  7:196739/1‑104 (MQ=255)                                                                                                                 ccTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTcc                                                                         <  8:196739/104‑1 (MQ=255)                                                                                                                    gtgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGTTAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTgg                                                        <  2:50555/118‑1 (MQ=255)                                                                                                                    gtgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGCAACCACAATTgg                                                        >  1:50555/1‑118 (MQ=255)                                                                                                                                cACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCggg             >  1:233951/1‑149 (MQ=255)                                                                                                                                 aCGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGc            >  4:78801/1‑149 (MQ=255)                                                                                                                                    cgGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTAc         <  2:233951/149‑1 (MQ=255)                                                                                                                                           cgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAc  <  7:82510/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                GCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAC  >  NC_000913/1261154‑1261440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑