| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 272,330 | 1 | . | G | 20.0% | 126.1 / 45.8 | 50 | intergenic (+141/‑250) | insN/eyeA | pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/novel sRNA, function unknown, CP4‑6 propahge |
| Reads supporting (aligned to +/- strand): ref base . (19/21); new base G (5/5); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCTATAGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCA > NC_000913/272184‑272473 | aCCTATAGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑g < 2:38325/148‑1 (MQ=255) cTATAGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCa > 7:104108/1‑149 (MQ=255) gtgtGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGGCTGTCTGGAATAGTGAACCGGTAACTCACGCTGAGAACCCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGcc < 3:91138/148‑1 (MQ=255) ggAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGGGGAGAACCTGAGCGGCAGTGACCTGCGGGCATG‑CCGCAggtca < 4:51095/149‑4 (MQ=255) gAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATG‑CCGCAggtcac > 4:139363/1‑145 (MQ=255) aGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATG‑CCGCAggtcact < 3:139363/149‑6 (MQ=255) aGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGAt < 8:91925/149‑1 (MQ=255) ttCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATGccgcag < 6:18723/141‑5 (MQ=255) ttCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATGccgcag > 5:18723/1‑137 (MQ=255) ggcgggcATGGATGCTTAAATGAACCGCAAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAAcc < 6:29013/148‑1 (MQ=255) aTGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATGccgcag > 5:145629/1‑121 (MQ=255) aTGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATGccgcag < 6:145629/125‑5 (MQ=255) gCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATG‑CCGCAggtca > 3:60456/1‑124 (MQ=255) gCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATG‑CCGCAggtca < 4:60456/127‑4 (MQ=255) cTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGc > 6:24449/1‑137 (MQ=255) cTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGc < 5:24449/137‑1 (MQ=255) ttAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTCATGTAACCCGCTGACACAGGGGa > 6:46196/1‑148 (MQ=255) cgcgAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCgaga < 6:91572/149‑1 (MQ=255) gcgAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGAt < 7:109846/149‑1 (MQ=255) tctgGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGcc > 1:131224/1‑149 (MQ=255) tctgGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGcc > 4:32001/1‑149 (MQ=255) tgGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATc > 8:133603/1‑139 (MQ=255) tgGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATc < 7:133603/139‑1 (MQ=255) gAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCgaga < 2:131224/149‑1 (MQ=255) tatTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCga < 6:12070/130‑1 (MQ=255) cGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAg > 5:55182/1‑149 (MQ=255) gTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTaa > 8:47303/1‑129 (MQ=255) gTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTaa < 7:47303/129‑1 (MQ=255) cTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAg < 3:98638/149‑1 (MQ=255) cTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAg > 4:98638/1‑149 (MQ=255) tCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAg > 8:18849/1‑111 (MQ=255) tCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAg < 7:18849/111‑1 (MQ=255) aCGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCAc > 5:155853/1‑119 (MQ=255) aCGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCAc < 6:155853/119‑1 (MQ=255) agaAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGaaa < 8:104108/148‑1 (MQ=255) gaAACCCAACAATCCCAGCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGATATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAgg < 5:46196/149‑1 (MQ=255) aacaaTCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTgaag > 4:72631/1‑148 (MQ=255) aaTCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACa < 6:55182/149‑1 (MQ=255) aTCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACt < 1:104213/102‑1 (MQ=255) aTCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACt > 2:104213/1‑102 (MQ=255) tCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACAt > 3:73939/1‑148 (MQ=255) cACCTGGTGTCAAGGAGAAGAACCTTAGCGGCAGTGACCTGC‑GGCATGCCCTCAGGATGATGTAACCCTCAGACAACGGGGATTTAGTCGAGATCACTAAGCCGATATGATc > 4:106199/1‑112 (MQ=255) cACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAAc > 2:61453/1‑149 (MQ=255) gTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCct < 4:73939/149‑1 (MQ=255) ggAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCctct < 3:32001/149‑1 (MQ=255) gagaACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGt > 3:124843/1‑103 (MQ=255) gagaACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGt < 4:124843/103‑1 (MQ=255) cTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGtt > 8:152004/1‑149 (MQ=255) cccTGCGGGCATG‑CCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTcacca > 4:37668/2‑149 (MQ=255) tGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTa > 3:79632/1‑116 (MQ=255) tGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTa < 4:79632/116‑1 (MQ=255) | ACCTATAGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCA > NC_000913/272184‑272473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |