Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,073,852 G→A 5.2% Q54* (CAA→TAA)  rutA ← pyrimidine oxygenase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,073,8520GA 5.2% 253.0 / 2.4 77Q54* (CAA→TAA) rutApyrimidine oxygenase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base G (40/33);  new base A (2/2);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

TAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGAAAGTCAGACGGGGCG  >  NC_000913/1073704‑1073998
                                                                                                                                                    |                                                                                                                                                  
tAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTg                                                                                                                                                    <  6:309970/149‑1 (MQ=255)
tAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTg                                                                                                                                                    <  2:23602/149‑1 (MQ=255)
   aTCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGca                                                                                                                                                  >  2:356048/1‑148 (MQ=255)
    tCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGcac                                                                                                                                                 >  3:351974/1‑148 (MQ=255)
        aaTGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                                                                                                                                               <  4:159572/146‑1 (MQ=255)
        aaTGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATgg                                                                                                                                            >  8:53233/1‑149 (MQ=255)
        aaTGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATgg                                                                                                                                            >  7:38164/1‑149 (MQ=255)
               gAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGCTCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt                                                                                                                                     <  1:194672/149‑1 (MQ=255)
               gAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt                                                                                                                                     <  3:24102/149‑1 (MQ=255)
                aGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt                                                                                                                                     >  1:284920/1‑148 (MQ=255)
                  gTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTACACAATGGCTTTATTCAg                                                                                                                                  >  8:230131/1‑149 (MQ=255)
                    cACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCGTTATTCAGtt                                                                                                                                <  8:314177/149‑1 (MQ=255)
                     aCGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGGAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTACGCTTTTTGCACAAAGGCATTATTCAGtt                                                                                                                                >  4:64807/1‑148 (MQ=255)
                     aCGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTc                                                                                                                               <  7:278621/149‑1 (MQ=255)
                      cGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                                                                                                                                               >  6:386598/1‑132 (MQ=255)
                      cGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                                                                                                                                               <  5:386598/132‑1 (MQ=255)
                           gccAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGCTCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGt                                                                                                                         >  3:344968/1‑149 (MQ=255)
                               gCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGGAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGc                                                                                                                     <  3:64807/149‑1 (MQ=255)
                               gCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGc                                                                                                                     <  7:254650/149‑1 (MQ=255)
                                   cGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGt                                                                                                                 <  1:2426/149‑1 (MQ=255)
                                        tCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTgc                                                                                                            >  7:383428/1‑149 (MQ=255)
                                              tGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTACACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGcgcg                                                                                                      <  7:230131/149‑1 (MQ=255)
                                                aaCGAATCCAGGTCGTGATCCCAGACCTCACTTTAGCCGCCAAAGCCACGCAGTTTGATCATCGACACGTCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCATAATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTg                                                                                                    <  6:247564/149‑1 (MQ=255)
                                                 aCGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTgg                                                                                                   <  4:332728/149‑1 (MQ=255)
                                                     cTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGACAAAGCCACGCAGTTTGATCATCGAAAGGGCGAAATCGAAATGGTAGTGATCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGAGGGTc                                                                                                >  1:54395/1‑148 (MQ=255)
                                                      tCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt                                                                                                                                     >  3:250357/1‑110 (MQ=255)
                                                      tCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt                                                                                                                                     <  4:250357/110‑1 (MQ=255)
                                                      tCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCg                                                                                               >  8:179930/1‑148 (MQ=255)
                                                        aaGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGATATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTTAAAGGGCGGCATGTACTGCGGCGCGTGGGTCGaaa                                                                                            <  2:120743/149‑1 (MQ=255)
                                                        aaGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCtt                                                                                                                                         >  5:155944/1‑104 (MQ=255)
                                                        aaGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCtt                                                                                                                                         <  6:155944/104‑1 (MQ=255)
                                                        aaGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGaaa                                                                                            <  4:351974/149‑1 (MQ=255)
                                                        aaGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGaaa                                                                                            >  8:83566/1‑149 (MQ=255)
                                                        aaGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGaaa                                                                                            >  1:259696/1‑149 (MQ=255)
                                                        aaGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGaa                                                                                             <  3:251029/148‑1 (MQ=255)
                                                        aaGGTTGTGATCCCAGAACTCAGTATTGCCGCCAAAGCCACGAAGTTTGATCATCGACAGGGCGAAATCTAAATGGTAGTGATACGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGaaa                                                                                            >  4:360488/1‑149 (MQ=255)
                                                          ggTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTg                                                                                                    <  7:242931/139‑1 (MQ=255)
                                                          ggTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTg                                                                                                    >  8:242931/1‑139 (MQ=255)
                                                               tgATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGagcca                                                                                     <  1:111184/149‑1 (MQ=255)
                                                                    ccAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCgt                                                                                >  8:64404/1‑149 (MQ=255)
                                                                        aaCTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCgttgtt                                                                            >  1:241728/1‑149 (MQ=255)
                                                                          cTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGc                                                                          >  4:370394/1‑149 (MQ=255)
                                                                          cTCAGTGTTGCCGCCACAGACACGCAGTTTGATCATCGAGAGGGCGAAATAGAAATTGTCGTGCTCAGCTTTTTGCACAATGGCTTTATTCATTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGc                                                                          <  5:267837/149‑1 (MQ=255)
                                                                           tCAGTTTTGCCCCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTATTGCACAATGGCTTTATTCAGTACAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGc                                                                          >  4:165516/1‑148 (MQ=255)
                                                                              gTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCaa                                                                       >  5:293445/1‑148 (MQ=255)
                                                                              gTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAAt                                                                      <  8:383428/149‑1 (MQ=255)
                                                                                    ccgccAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTAc                                                                <  7:53233/149‑1 (MQ=255)
                                                                                       ccAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGaa                                                             <  7:179930/149‑1 (MQ=255)
                                                                                               aCGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCaa                                                     >  6:319978/1‑149 (MQ=255)
                                                                                                 gCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt                                                                                                                                     <  6:147817/67‑1 (MQ=255)
                                                                                                 gCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTAtt                                                                                                                                     >  5:147817/1‑67 (MQ=255)
                                                                                                  cAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAAttt                                                  >  2:348212/1‑149 (MQ=255)
                                                                                                    gTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAAtttt                                                 >  7:220631/1‑148 (MQ=255)
                                                                                                    gTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTtc                                                <  7:136622/149‑1 (MQ=255)
                                                                                                              cGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAAcc                                      >  2:184130/1‑149 (MQ=255)
                                                                                                               gACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACct                                     >  2:376447/1‑149 (MQ=255)
                                                                                                                aCAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACACTGGCTTTATTGAGTTCAAAGGTCGGCATGTACTGCGGCGCGGGGGTCGAAATGAGCCCGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCCTCCACctg                                    >  4:307770/1‑148 (MQ=255)
                                                                                                                aCAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACctc                                    <  1:356048/149‑1 (MQ=255)
                                                                                                                aCAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACctc                                    >  8:61460/1‑149 (MQ=255)
                                                                                                                   gggCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCCTGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACctctct                                 >  7:136375/1‑149 (MQ=255)
                                                                                                                    ggCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCtt                                <  8:38164/149‑1 (MQ=255)
                                                                                                                     gCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTc                               >  7:117263/1‑149 (MQ=255)
                                                                                                                         aatcgaaatGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGtct                           >  1:101364/1‑149 (MQ=255)
                                                                                                                              aaatGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTa                       >  3:105090/1‑148 (MQ=255)
                                                                                                                              aaatGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGAGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTa                       >  7:374478/1‑148 (MQ=255)
                                                                                                                               aatGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCATAGGTCTGCATGTACTGCGGCGCGTGGGGCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTaaa                     <  6:375314/149‑1 (MQ=255)
                                                                                                                                   gTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGGTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGa                 <  2:101364/149‑1 (MQ=255)
                                                                                                                                    tAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGa                 >  6:282831/1‑148 (MQ=255)
                                                                                                                                      gTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGa                 >  6:285760/1‑146 (MQ=255)
                                                                                                                                      gTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGa                 <  5:285760/146‑1 (MQ=255)
                                                                                                                                       tGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGAAAGt             >  2:332261/1‑149 (MQ=255)
                                                                                                                                       tGCTCCGCTTTTTACACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGa                 <  1:267896/145‑1 (MQ=255)
                                                                                                                                       tGCTCCGCTTTTTACACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGa                 >  2:267896/1‑145 (MQ=255)
                                                                                                                                         cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGAAAGt             >  6:221620/1‑147 (MQ=255)
                                                                                                                                           ccGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGTAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTGTTCGTCTCGTAAAGTGAAAGTCAGa         <  2:46478/149‑1 (MQ=255)
                                                                                                                                            cGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGAAAGTCAGa         <  3:370394/148‑1 (MQ=255)
                                                                                                                                            cGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGAAAGTCAGAc        <  1:376447/149‑1 (MQ=255)
                                                                                                                                            cGCTTTTTGCACAATGGCTTTATGCACTTCAAAGGTCGGCATGTACTGCGGCGCGCGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGCACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGAAAGTCAGa         <  1:128563/148‑1 (MQ=255)
                                                                                                                                                  ttGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGAAAGTCAGACGGGgcg  >  2:94726/1‑149 (MQ=255)
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TAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCAATTTTCATCATCAACCTCTCTTCGTCTCGTAAAGTGAAAGTCAGACGGGGCG  >  NC_000913/1073704‑1073998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: