Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,261,297 G→T 100% D193E (GAC→GAA prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,261,2970GT79.2% 155.6 / 50.2 82D193E (GAC→GAAprsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (8/9);  new base T (27/38);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.51e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACG  >  NC_000913/1261157‑1261441
                                                                                                                                            |                                                                                                                                                
ccacGTTCTTTCAGAGCTTCAGCAGCTTTACATAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCgatgat                                                                                                                                          <  6:71648/149‑1 (MQ=255)
ccacGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTGGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCgatgat                                                                                                                                          <  5:150726/149‑1 (MQ=255)
ccacGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCgatgat                                                                                                                                          >  8:174506/1‑149 (MQ=255)
    gTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGcc                                                                                                                                      <  3:43547/149‑1 (MQ=255)
    gTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGc                                                                                                                                       >  3:127577/1‑148 (MQ=255)
          tCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCata                                                                                                                                   <  7:277354/146‑1 (MQ=255)
             gagCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTa                                                                                                                             >  1:84405/1‑149 (MQ=255)
              agCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTAt                                                                                                                            >  2:377044/1‑149 (MQ=255)
                    agcagcTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTca                                                                                                                      <  3:167720/149‑1 (MQ=255)
                    agcagcTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTca                                                                                                                      <  8:202624/149‑1 (MQ=255)
                         cTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCagcagc                                                                                                                 <  1:377044/149‑1 (MQ=255)
                            tACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACTACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCtt                                                                                                               <  2:57972/148‑1 (MQ=255)
                              cacaGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGc                                                                                                            <  2:243635/149‑1 (MQ=255)
                                caGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGa                                                                                                          <  8:34905/149‑1 (MQ=255)
                                  gCGTACCGCCTGTGTCGATCATATCATCGACCAGTACACAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATa                                                                                                        <  7:185412/149‑1 (MQ=255)
                                  gCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACTCGGAAGACGTTTTTCGATGATTTCCATATCGGTATCGTTCAGCAGCTTAGCGATa                                                                                                        <  4:145301/149‑1 (MQ=255)
                                  gCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATa                                                                                                        <  5:217246/149‑1 (MQ=255)
                                       ccGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGcgcg                                                                                                   >  7:273351/1‑149 (MQ=255)
                                       ccGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGcgcg                                                                                                   <  4:261127/149‑1 (MQ=255)
                                        cGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCgg                                                                                                  >  5:40877/1‑149 (MQ=255)
                                         gCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGCCGTTTGTCGATGATTGCCATATCGGTAGCGTTCAGCAGCTTAGCGATAGCGCggg                                                                                                 >  1:208924/1‑149 (MQ=255)
                                         gCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCggg                                                                                                 <  7:357435/149‑1 (MQ=255)
                                               gtCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGgcacgca                                                                                           >  7:322789/1‑149 (MQ=255)
                                                tCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGgcacgcac                                                                                          <  8:174947/149‑1 (MQ=255)
                                                  gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACaa                                                                                        <  5:350875/149‑1 (MQ=255)
                                                  gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACaa                                                                                        >  6:350875/1‑149 (MQ=255)
                                                   atcatatcatCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAc                                                                                       <  4:291750/149‑1 (MQ=255)
                                                       tatcatCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAcgccg                                                                                   >  6:102009/1‑149 (MQ=255)
                                                       tatcatCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAcgccg                                                                                   >  8:351938/1‑149 (MQ=255)
                                                        atcatcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAcgccgc                                                                                  >  8:104280/1‑149 (MQ=255)
                                                         tcatcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAcgccgc                                                                                  <  6:88310/148‑1 (MQ=255)
                                                         tcatcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACgccgcc                                                                                 <  8:74265/149‑1 (MQ=255)
                                                            tcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGAt                                                                              <  8:273351/149‑1 (MQ=255)
                                                                ccAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGAt                                                                                                         <  8:61077/118‑1 (MQ=255)
                                                                ccAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGAt                                                                                                         >  7:61077/1‑118 (MQ=255)
                                                                   gTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGga                                                                       <  7:174506/149‑1 (MQ=255)
                                                                    tACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGtt                                                                                                                        >  7:87394/1‑99 (MQ=255)
                                                                    tACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGtt                                                                                                                        <  8:87394/99‑1 (MQ=255)
                                                                    tACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATa                                                                                                        <  1:362230/115‑1 (MQ=255)
                                                                    tACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATa                                                                                                        >  2:362230/1‑115 (MQ=255)
                                                                    tACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCg                                                                         >  6:112914/1‑146 (MQ=255)
                                                                      cGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGgaga                                                                     <  5:8625/148‑1 (MQ=255)
                                                                       gCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGaaa                                                                   <  8:190396/149‑1 (MQ=255)
                                                                       gCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGaaa                                                                   <  7:100938/149‑1 (MQ=255)
                                                                        cAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAAc                                                                  <  3:81998/149‑1 (MQ=255)
                                                                          gTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACca                                                                >  5:73943/1‑149 (MQ=255)
                                                                           tCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACCACGCCGCCGATGTCCGGAGAAACca                                                                >  4:374009/1‑148 (MQ=255)
                                                                             aCGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAGACCACaa                                                             >  2:182453/1‑149 (MQ=255)
                                                                              cGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAAt                                                            >  5:388479/1‑149 (MQ=255)
                                                                               gACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGCTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAAtt                                                           >  4:322709/1‑149 (MQ=255)
                                                                                aCCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTg                                                          <  7:224806/149‑1 (MQ=255)
                                                                                 ccTGCACCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGGTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTgg                                                         <  8:401954/149‑1 (MQ=255)
                                                                                 ccTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGt                                                                            <  2:257270/130‑1 (MQ=255)
                                                                                 ccTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGt                                                                            >  1:257270/1‑130 (MQ=255)
                                                                                 ccTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAGACCACAATTgg                                                         <  1:182453/149‑1 (MQ=255)
                                                                                 ccTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTgg                                                         <  3:354301/149‑1 (MQ=255)
                                                                                 ccTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTgg                                                         <  2:84405/149‑1 (MQ=255)
                                                                                  cTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTggg                                                        <  3:321908/149‑1 (MQ=255)
                                                                                  cTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTggg                                                        <  8:126255/149‑1 (MQ=255)
                                                                                   tGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGt                                                       >  4:36421/1‑149 (MQ=255)
                                                                                     cAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGtt                                                      <  5:102009/148‑1 (MQ=255)
                                                                                     cAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGt                                                       <  6:73943/147‑1 (MQ=255)
                                                                                       aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGTTTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATc                                                   <  7:34399/149‑1 (MQ=255)
                                                                                         gTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAACCCACAATTGGGTTATc                                                   >  2:162729/1‑147 (MQ=255)
                                                                                         gTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCa                                                 <  4:17415/149‑1 (MQ=255)
                                                                                                atgatATGCATCACCTGTGAAACGCTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAg                                          <  3:322709/149‑1 (MQ=255)
                                                                                                          tCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATg                                >  7:405533/1‑149 (MQ=255)
                                                                                                              cTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCtt                            >  5:180533/1‑149 (MQ=255)
                                                                                                              cTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCtt                            >  8:243048/1‑149 (MQ=255)
                                                                                                                 tgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTc                           >  3:222510/1‑147 (MQ=255)
                                                                                                                 tgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTc                           >  7:285162/1‑147 (MQ=255)
                                                                                                                 tgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTc                           >  3:191696/1‑147 (MQ=255)
                                                                                                                       gTTCGCACGCGGACGACGTTTTTCGATGATTGACATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGGTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGa                   <  8:405100/149‑1 (MQ=255)
                                                                                                                            cACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCggg              >  1:54409/1‑149 (MQ=255)
                                                                                                                             aCGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGc             >  6:55290/1‑149 (MQ=255)
                                                                                                                             aCGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACACCGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGc             >  3:200995/1‑149 (MQ=255)
                                                                                                                                    cgacgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCaaa      <  6:180533/149‑1 (MQ=255)
                                                                                                                                     gacgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCaaa      >  7:44648/1‑148 (MQ=255)
                                                                                                                                     gacgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAAt     <  6:40877/149‑1 (MQ=255)
                                                                                                                                     gacgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAAt     <  7:104280/149‑1 (MQ=255)
                                                                                                                                     gacgTATTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCaaa      >  2:230297/1‑148 (MQ=255)
                                                                                                                                        gTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCCGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACg  <  3:247654/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACG  >  NC_000913/1261157‑1261441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: